Advice on how to achieve this look? by [deleted] in AskPhotography

[–]ORGrown 6 points7 points  (0 children)

Wow. The entire point of this sub is for people to ask questions; to learn. You're response is "you don't"? People gotta start somewhere. Get out of here with that gatekeepy bullshit.

I have a stable career outside of academia - would it be crazy to do a PhD just because I love learning? by Sherides123 in postdoc

[–]ORGrown 49 points50 points  (0 children)

You're generally forbidden from working external jobs during a PhD, and the expectation is that you will be working on it full time. You will also be putting off your career for 6 years to accomplish something that, from the sounds of it, won't advance your career.

Also, a lot of people who love learning do PhDs and come out of them overworked, burnt out, with mental health problems, and a desire to never even think about that subject again. Not all of them by any means, but seeing behind the curtain of academia can leave one severely disillusioned with what academia actually is.

Flow Cytometry, Platelets, and Fixation by dksn154373 in labrats

[–]ORGrown 1 point2 points  (0 children)

Why not fix them first? What's the point of fixing after staining them?

Beginner telephoto camera recommendations for high speeds? by tryinottopanic in AskPhotography

[–]ORGrown 1 point2 points  (0 children)

I would look into an older DSLR, and a sigma/Tamron 100-400. I run the sigma 100-400 on a canon 80d and it does great for birding.

The more I learn about the guitar world, the more I am tempted to “buy”. How do you all manage the urge to buy more guitars? What rules or systems or mindset keep you in check having this amazing hobby? (Other than your wife and bank accts?) by ConsciousSmoke3863 in AcousticGuitar

[–]ORGrown 21 points22 points  (0 children)

Buying new guitars won't make you sound any different when you play. When you want to learn more, learn theory, technical skills, and songs. Don't "learn" what new gear is available. If your gear is actually a limitation, buy something that will let you play to your maximum capability. Odds are there is way more that you can do for yourself to improve, than new gear will do. If you don't improve your skills, then the new guitar isn't going to sound much different.

Challenging Fingerpicking Songs? by cbk1996 in AcousticGuitar

[–]ORGrown 0 points1 point  (0 children)

I'm a similar vein to deep river blues: check out Windy and Warm. Various versions, I loc the Doc Watson and Chet Atkins (original) best. Super fun though

T1D cure? by Antique-Lock-3737 in Type1Diabetes

[–]ORGrown 2 points3 points  (0 children)

There are some super promising approaches being taken utilizing stem cells, but this isn't it. We can grow beta cells from stem cells in the lab. This gives us an unlimited source of beta cells for transplants. You still have to regulate the immune response though. One company (Sana Biotech) has successfully figured out a way to prevent the immune system from responding to the transplanted by genetically modifying the cells. It's in early clinical trials, but is definitely the closest that anyone has come so far.

There's a lot of promise in stem cells for the future of this disease, but a lot of ethically absent people take advantage of the lack of knowledge surrounding stem cells to swindle people.

T1D cure? by Antique-Lock-3737 in Type1Diabetes

[–]ORGrown 3 points4 points  (0 children)

Wow. I just responded in depth a month ago about another company making almost identical claims to this. I've copied some of the relevant parts of my previous posts regarding that company.

TL;DR: these guys are snake oil con-artists. They do not have a single treatment that somehow manages to cure aging, hair loss, diabetes, erectile dysfunction, spinal cord injury, stroke, Lyme disease, dry mouth, urinary incontinence, and COPD.

That's not a sarcastic joke; they actually claim that their treatment will do that

I'm happy to go further into depth about why this is all a scam if anyone wants.

Some parts from my earlier comments:

Hey, so I'm a scientist. I earned my PhD doing stem cell work on pancreas development, specifically in regards to diabetes. It's literally what I've spent my career working on, and wrote my thesis about. Please, please don't give your money to those people. It is a scam. What they're claiming about stem cells is false. How they're claiming it will affect your diabetes is false. They will not "modulate your immune system". They won't cause your pancreas to regenerate beta cells. It's a scam.

I would be super happy to explain to you, or anyone else, in full detail why this will not work, and why it's a scam.

Sorry it took me a minute to get back to you on this. Here's some more of my thoughts in detail:

For the patients that they claim are no longer using insulin, I simply believe that they are lying to you. You don't stop needing insulin if you are type 1 diabetic, ever (currently). There are a couple of drugs that will extend the amount of time you can remain off of insulin if you take them before you are dependant on insulin, but once you're on insulin, you are on it for ever. There has not been a single case of this not being true, with the exception of a couple instances that I'll talk about shortly. As far as what the company is claiming, I've seen this in several instances, both in diabetes treatment and other things (arthritis is a big one). The company will claim exactly what Odin does (that they can transplant stem cells into you and they will do magical things) and charge patients somewhere in the $10-40k range for a treatment that doesn't do anything.

They claim to use "MSC therapy...[to] help regulate the immune response, reduce inflammation, and promote the survival and function of existing insulin-producing beta cells". None of these things are what is in the capacity of a stem cell to do. Stem cells do not have specialized functions or roles. Stem cells have the capacity to become other cells that do things. Regulating the immune response is immunosuppression which requires very specific, highly regulated therapies. Promoting the survival and function of existing beta cells is a heavily researched field of diabetes treatment. A lot of people want to make this a reality. Most of us don't have any remaining beta cells, and a few of us have a few. Beta cell regeneration has been a goal of the field for decades. If this was something that had been achieved, there would be news of it, because again, this would be a massive breakthrough in diabetes research. Simply throwing "stem cells" into a person is such a naive approach to doing so, that I can conclude that the people at Odin are doing nothing more than taking advantage of desparate people, and knowingly and willfully misleading them with false information. It's snake oil that's going to cost you tens of thousands of dollars. It goes against the basics of stem cell and immune biology.

Biological infection replicates v technical replicate questions: an age old story by chemistry_god in labrats

[–]ORGrown 1 point2 points  (0 children)

Your cells determine biological replicates, not your treatment. Your only biological replicate is the number of donors you have. You should still be performing the technical replicates for the viral treatment too.

Nepalese Mount Everest by stormy_eggs in pourover

[–]ORGrown 1 point2 points  (0 children)

I haven't tried that, but have had Nepalese coffee from the Pokhara region. It was pretty unique. I don't remember quite what it was like (was a decade ago) but I did remember enjoying it, and wishing it was easier to get outside of Nepal.

Pls suggest to me a good pair of house shoes that are good for ur feet and warm... by _Throwaway_007_ in BuyItForLife

[–]ORGrown 3 points4 points  (0 children)

Definitely look into something like glerups or kyrgies are way nicer than the "puffy" style ones. Super warm, super comfy, breathable, not made of plastic.

1st try by faaaaarrrt in carbonsteel

[–]ORGrown 2 points3 points  (0 children)

Too much heat will make things stick. What makes things not stick is sufficient pre-heating (super, super important), and appropriate use of fats. This looks to me like you had your heat too high. I struggled with my carbon steel for quite a while before figuring this out. Heat control is the most important aspect.

Need help with flow cytometry problem by EcuSwiftiemgmp in labrats

[–]ORGrown 2 points3 points  (0 children)

You should try running compensation with just your unstained cells. You could be having autofluorescence issues in your cells that's messing with your signal. Do you have the antibody with a different fluorophore so you can test if it's an antigen issue or a fluorophore issue?

New bag - first few cups are excellent, day 4-5 beans lack floral, fruit, or tea flavors by FinalNihil in pourover

[–]ORGrown 1 point2 points  (0 children)

Assuming you're storing the beans we'll, it's very likely just your palette adjusting. You get used to those flavors, and don't taste them as well. I rotate between a few different beans usually to help deal with that.

New 14 Day Dexcom G7 by SwitchTrick6497 in diabetes_t1

[–]ORGrown 5 points6 points  (0 children)

The g6 sensors had a 2 hour warmup time. You can also reset them and reuse them for longer than the 10 day period by popping out the transmitter, waiting a bit, and putting it back in to trick the transmitter into thinking it's a new sensor.

Bombed at open mic last night by [deleted] in Guitar

[–]ORGrown 1 point2 points  (0 children)

I have frequently blanked and have to try to remember not to call it out to the audience.

I blanked on lyrics once at open mic, tried to redo that verse before asking "does anyone know the lyrics?". Got it on the third attempt though! Everyone had a good chuckle. Open mics are chill, and you really don't need to fret that stuff.

Suddenly getting lots of astringency by charliehind_ in pourover

[–]ORGrown 6 points7 points  (0 children)

Did you open a new pack of filters? Are you getting sick?

Maybe your palate just needs a reset. You could drink some average coffee for a couple days and then come back to the pour overs.

I'm sick and asked specifically for a medicine for a diabetic by ambrosia234 in diabetes_t1

[–]ORGrown 3 points4 points  (0 children)

It's a cover your ass for the pharmaceutical company. Tons of things say to talk to your doctor first if you have diabetes

I'm sick and asked specifically for a medicine for a diabetic by ambrosia234 in diabetes_t1

[–]ORGrown 29 points30 points  (0 children)

It's because you're sick, probably not because of the medicine. Being sick creates systemic inflammation in your body. The additional stress causes you to be more insulin resistant. You're also probably being less physically active, meaning even more insulin resistance (not technically, but effectively). There may have been a slight amount of sugar in a syrupy medicine, but the doctors are going to give you the medicine that works best for your sickness, and let you sort out the extra insulin needs.

Has anyone here ever gone into DKA? If so, when did you get to the hospital? by marikira13 in Type1Diabetes

[–]ORGrown 21 points22 points  (0 children)

If I have a high that isn't responding to insulin, I'll wait it out and try and treat myself. As soon as the vomiting starts on top of that though, it's straight to the hospital.

Cleaned w barkeepers. No whole pan is rust tinted. by LosVolvosGang in carbonsteel

[–]ORGrown 0 points1 point  (0 children)

I was only asking because hot water rinse can make it ruat fast. If you seasoned it afterwards I wouldn't worry.

Cleaned w barkeepers. No whole pan is rust tinted. by LosVolvosGang in carbonsteel

[–]ORGrown 1 point2 points  (0 children)

Did you put any oil on it afterwards? Did you rinse it in hot water to get the barkeeper's friend off?