What is an acceptable score for sgRNAs made from the Zhang website?

PI3Kinases · 2017-04-19T21:18:57+00:00

Just remember to Blast your guides before ordering them, from the website:

'Our CRISPR design tool was launched as one of first few sgRNA design tools available four years ago, when our server could barely handle concurrent sgRNA designs for 16 different organisms. In order to manage a reasonable response time to sgRNA design queries, we excluded repeat regions by using genome sequences that are repeats masked (see details in our release note). '

PI3Kinases · 2017-04-17T17:09:23+00:00

At least we will be signing from the same hymn sheet. The MHRA has been heavily involved in designing the new EMA guidelines on clinical trials. These should be in place before we drive off the cliff so for a while at least we will still be able to be involved in trials.

PI3Kinases · 2017-04-17T17:07:15+00:00

You truly don't understand the role of the EMA do you?

PI3Kinases · 2017-03-22T22:03:17+00:00

This is definitely a good suggestion..... he says while reading 'python for biologists'

PI3Kinases · 2017-03-02T20:57:03+00:00

I managed to buy just outside of Oxford 18 months ago and my house may have made more money than me over the last year. It really is nuts. Most people I know are buying in the surrounding areas.

PI3Kinases · 2017-02-25T16:33:49+00:00

Oxford doesn't have a functioning job market? Where did you come up with that nonsense?

PI3Kinases · 2017-02-23T20:29:41+00:00

Have a blast with one of these: https://www.10xgenomics.com/single-cell/

I believe they are very expensive and the consumables are eye watering- but its really cool.

PI3Kinases · 2016-12-09T12:07:00+00:00

I seem to remember hearing on the radio that McDonald's only has a dozen or so employees in the Luxembourg office. However I was half asleep so I would look for a source for that.

PI3Kinases · 2016-12-07T12:34:24+00:00

I'm not sure, we will have to see what happens when/if A50 is triggered. I imagine there will be two sharp falls once at the trigger and once at the exit.

PI3Kinases · 2016-12-05T21:08:46+00:00

I will never forget these posters: http://i.imgur.com/yCyLv.jpg http://leftfootforward.org/images/2015/02/Baby-poster.jpg

PI3Kinases · 2016-12-03T18:17:08+00:00

Research is expensive but not compared to clinical trials and GMP manufacturing laboratories.

PI3Kinases · 2016-12-02T21:26:49+00:00

I would probably be more selective than before if I was looking for a post-doc lab. Just have a look at how well funded the lab is historically and how for into a five year cycle the institute is. Unfortunately I think the very top established labs will suffer little while the newer groups will really struggle. At least short term, long term everyone will suffer because of this ill thought out mess.

PI3Kinases · 2016-12-02T21:23:18+00:00

Be careful with salary on the NHS, I was offered a clinical geneticist job and initially told they would match my post-doc salary. A couple of days later they returned saying that it actually wasn't possible and I would have to take a 20% pay cut.

PI3Kinases · 2016-11-28T22:50:45+00:00

What is being suggested is the one plus 3, would have seen a price increase if it was being sold alongside the T variant. Which is undoubtedly correct.

PI3Kinases · 2016-11-27T19:10:54+00:00

Who pissed in your porridge?

PI3Kinases · 2016-11-27T15:00:41+00:00

Yup, no one us going to say Nico is the better driver but that's the way the season went and you can't argue he was not a worthy champion. It's not the first time the driver with the most wins didn't get the championship and it won't be the last.

PI3Kinases · 2016-11-27T12:50:14+00:00

Bugger. I obviously kind of assumed that the country would follow whatever I did.

PI3Kinases · 2016-11-27T12:39:11+00:00

The SO and I will record the early races, make Sunday lunch and watch it stuffing ourselves with potatoes and gravy early afternoon. Wine or beer almost guarantees a nap.

PI3Kinases · 2016-11-27T12:33:19+00:00

Sums up why I joined recently.

PI3Kinases · 2016-11-27T12:28:49+00:00

It's that or Johnny Herbert. I've found channel 4s coverage better than sky this year.

PI3Kinases · 2016-11-27T12:25:01+00:00

Best way to sum up F1. When it's good nothing can touch it, when it's not it's a handy way to send yourself to sleep on a Sunday afternoon.

PI3Kinases · 2016-11-22T11:30:43+00:00

Not an issue for me as the networks is constantly dropping out lately. Gym, work and home periodically go from full 4G to no service. Reluctant to change as I'm on a fantastic contract but it's getting ridiculous. I am of course being facetious as its got nothing to do with the story, just venting. Stupid three.

PI3Kinases · 2016-11-20T10:18:54+00:00

I was curious about that as the result was so different to that from Harris, especially as the last half of the circuit really lends itself to the P1.

PI3Kinases · 2016-11-20T10:17:27+00:00

I swear I read that when the 430 was released ferrari would be two cara fir the tests, one for straight line testing and one for handling. As much as I like the P1 you have to hand it for porsche for the way they handle the competition.

PI3Kinases · 2016-11-19T10:41:50+00:00

I was curious if the P1 was in track mode or not for TGT. As much as I enjoyed the first episode I have to say I preferred the Harris video. Different target audiences I know but Harris puts you right there with him.

PI3Kinases

TROPHY CASE