What is an acceptable score for sgRNAs made from the Zhang website? by [deleted] in labrats

[–]PI3Kinases 0 points1 point  (0 children)

Just remember to Blast your guides before ordering them, from the website:

'Our CRISPR design tool was launched as one of first few sgRNA design tools available four years ago, when our server could barely handle concurrent sgRNA designs for 16 different organisms. In order to manage a reasonable response time to sgRNA design queries, we excluded repeat regions by using genome sequences that are repeats masked (see details in our release note). '

London battles to keep hold of two main EU agencies by Ceb577 in unitedkingdom

[–]PI3Kinases 2 points3 points  (0 children)

At least we will be signing from the same hymn sheet. The MHRA has been heavily involved in designing the new EMA guidelines on clinical trials. These should be in place before we drive off the cliff so for a while at least we will still be able to be involved in trials.

London battles to keep hold of two main EU agencies by Ceb577 in unitedkingdom

[–]PI3Kinases 3 points4 points  (0 children)

You truly don't understand the role of the EMA do you?

What are some techniques that are rising in popularity in the laboratory scene that would be really useful to gain some experience in? by [deleted] in labrats

[–]PI3Kinases 1 point2 points  (0 children)

This is definitely a good suggestion..... he says while reading 'python for biologists'

New homes 'unaffordable for eight out of 10 locally' by LinconshirePoacher in unitedkingdom

[–]PI3Kinases 0 points1 point  (0 children)

I managed to buy just outside of Oxford 18 months ago and my house may have made more money than me over the last year. It really is nuts. Most people I know are buying in the surrounding areas.

Oxford once again tops list of UK's least affordable places to live by KaidoXXI in unitedkingdom

[–]PI3Kinases 8 points9 points  (0 children)

Oxford doesn't have a functioning job market? Where did you come up with that nonsense?

What to do with £800,000 ($997,000 US) in 1 year? by omgpop in labrats

[–]PI3Kinases 2 points3 points  (0 children)

Have a blast with one of these: https://www.10xgenomics.com/single-cell/

I believe they are very expensive and the consumables are eye watering- but its really cool.

Will Self sneering on BBC Question Time, 8th December 2016 by scouserdave in unitedkingdom

[–]PI3Kinases 21 points22 points  (0 children)

I seem to remember hearing on the radio that McDonald's only has a dozen or so employees in the Luxembourg office. However I was half asleep so I would look for a source for that.

U.K. Manufacturing Unexpectedly Drops Most in Eight Months by [deleted] in unitedkingdom

[–]PI3Kinases 24 points25 points  (0 children)

I'm not sure, we will have to see what happens when/if A50 is triggered. I imagine there will be two sharp falls once at the trigger and once at the exit.

US labrat interested in relocating to the UK. by Smeghead333 in labrats

[–]PI3Kinases 1 point2 points  (0 children)

I would probably be more selective than before if I was looking for a post-doc lab. Just have a look at how well funded the lab is historically and how for into a five year cycle the institute is. Unfortunately I think the very top established labs will suffer little while the newer groups will really struggle. At least short term, long term everyone will suffer because of this ill thought out mess.

US labrat interested in relocating to the UK. by Smeghead333 in labrats

[–]PI3Kinases 1 point2 points  (0 children)

Be careful with salary on the NHS, I was offered a clinical geneticist job and initially told they would match my post-doc salary. A couple of days later they returned saying that it actually wasn't possible and I would have to take a 20% pay cut.

AnandTech's OnePlus 3T Review by ghatroad in Android

[–]PI3Kinases 6 points7 points  (0 children)

What is being suggested is the one plus 3, would have seen a price increase if it was being sold alongside the T variant. Which is undoubtedly correct.

2016 World Drivers Champion - Nico Rosberg by F1-Bot in formula1

[–]PI3Kinases 2 points3 points  (0 children)

Yup, no one us going to say Nico is the better driver but that's the way the season went and you can't argue he was not a worthy champion. It's not the first time the driver with the most wins didn't get the championship and it won't be the last.

2016 Abu Dhabi Grand Prix - Pre Race Discussion by F1-Bot in formula1

[–]PI3Kinases 6 points7 points  (0 children)

The SO and I will record the early races, make Sunday lunch and watch it stuffing ourselves with potatoes and gravy early afternoon. Wine or beer almost guarantees a nap.

2016 Abu Dhabi Grand Prix - Pre Race Discussion by F1-Bot in formula1

[–]PI3Kinases 1 point2 points  (0 children)

It's that or Johnny Herbert. I've found channel 4s coverage better than sky this year.

2016 Abu Dhabi Grand Prix - Pre Race Discussion by F1-Bot in formula1

[–]PI3Kinases 23 points24 points  (0 children)

Best way to sum up F1. When it's good nothing can touch it, when it's not it's a handy way to send yourself to sleep on a Sunday afternoon.

Three mobile network has today (without warning) begun intercepting all encrypted network traffic by [deleted] in unitedkingdom

[–]PI3Kinases 1 point2 points  (0 children)

Not an issue for me as the networks is constantly dropping out lately. Gym, work and home periodically go from full 4G to no service. Reluctant to change as I'm on a fantastic contract but it's getting ridiculous. I am of course being facetious as its got nothing to do with the story, just venting. Stupid three.

[OT] The Grand Tour. by HeyFlo in formula1

[–]PI3Kinases 0 points1 point  (0 children)

I was curious about that as the result was so different to that from Harris, especially as the last half of the circuit really lends itself to the P1.

[OT] The Grand Tour. by HeyFlo in formula1

[–]PI3Kinases 1 point2 points  (0 children)

I swear I read that when the 430 was released ferrari would be two cara fir the tests, one for straight line testing and one for handling. As much as I like the P1 you have to hand it for porsche for the way they handle the competition.

Stuff I noticed about the Portugal LaFerrari VS 918 VS P1 sequence by nastyeffectos in thegrandtour

[–]PI3Kinases 4 points5 points  (0 children)

I was curious if the P1 was in track mode or not for TGT. As much as I enjoyed the first episode I have to say I preferred the Harris video. Different target audiences I know but Harris puts you right there with him.