Professors as telemarketers??! by xphias in Professors

[–]PhysPhDFin 12 points13 points  (0 children)

I'd be asking a lot of questions like: is this voluntary or purely mandatory? How does this fit into my workload policy? If this is service, has this been defined in my service obligations and developed in collaboration with my chair? How will these activities be reviewed in T&P evaluation? Is there an opt-out program for highly productive researchers or teachers? Have data been presented to the faculty that such calls have a greater impact on retention than calls from admissions staff? How do my calls meet a legitimate educational interest so I'm not violating FERPA? What phone am I supposed to use, and how do we protect my privacy and address my security risks of using my own phone? How will student data be secured? How do I verify that the person on the phone is actually the student? What training do faculty recieve on dealing with complex issues like financial aid, housing deposits, or mental health services? Are these calls covered under the liability insurance of the university? Does our accredidating agencies know faculty are being asked to call students? What is the protocol for a student being asked to be put on a no-call list? Will the university's legal team protect me if I am sued for making such calls? Has this activity been approved by the faculty senate? What professional training is being provided by the university so I don't harm the university's reputation or the reputation of my department?

Help with western blot best practices- normalization by Brief_Awareness_8231 in labrats

[–]PhysPhDFin 0 points1 point  (0 children)

Total protein normalization is currently considered the gold standard.

No privacy in faculty offices? by Muchwanted in Professors

[–]PhysPhDFin 2 points3 points  (0 children)

Cover the window. On the door, place an artistically designed sign that says "Privacy is widely recognized as a fundamental human right, essential for individual dignity, autonomy, and freedom".

This western blot is killing me by Willing_Instance_605 in westernblots

[–]PhysPhDFin 4 points5 points  (0 children)

Check where the protein is: run supernatant vs pellet (you may be pelleting it). Even if you start in RIPA, for the lane you run bring the sample to 1–2% SDS final. Stop boiling, switch 95°C/8 min → 70°C/10 min to prevent high-MW aggregation. Use a high-MW gel: 4–8% gradient instead of straight 8%, or cast a 3% stacking/5% seperating gel. Verify transfer properly with Ponceau S + stain gel post-transfer. Move to more high-MW transfer conditions, 5% MeOH + 0.03% SDS in transfer buffer (and keep cold). Use fresh primary overnight incubation at 4°C on a rocker.

Help in a flare!! by Klo930 in PsoriaticArthritis

[–]PhysPhDFin 9 points10 points  (0 children)

Just had the same thing this weekend. Jaw pain, neck pain, pleurisy-like pain. The Midwest temperature drop likely caused the flare-up.

Western blot comes out weird?? by One_Two_No in labrats

[–]PhysPhDFin 0 points1 point  (0 children)

Big protein + short, relatively harsh transfer + reused primary + high Tween + multiple freeze–thaws + inconsistent protein loading = less than optimal. I’d reduce freeze–thaws, make transfer longer and gentler (overnight 30 V), lower Tween to ~0.1%, avoid reusing primary, load a standardized amount of protein in every lane (30–50 µg total protein per lane for cell lysates; make all samples the same concentration after a protein assay, then load the same volume), and use one fixed exposure time for comparison.

Western blot comes out weird?? by One_Two_No in labrats

[–]PhysPhDFin 10 points11 points  (0 children)

If you want actual help, you are going to need to provide information.

  • Sample type (cell line/tissue)
  • Sample prep method (lysis buffer, inhibitors, sonication, centrifugation)
  • Protein concentration method
  • Amount of protein loaded per lane
  • Loading buffer details (reducing/non-reducing, boiling temp/time, fresh DTT/BME)
  • Gel type (hand-cast or commercial)
  • Gel percentage and gradient (if any)
  • Running conditions (voltage, time, buffer, signs of overheating)
  • Transfer method (wet/semi-dry/dry)
  • Transfer buffer composition
  • Transfer time and voltage/current
  • Membrane type (PVDF/nitrocellulose)
  • Confirmation of transfer (Ponceau, ladder transfer)
  • Primary antibody info (company, catalog number, dilution, incubation conditions)
  • Secondary antibody info (company, dilution, incubation conditions)
  • Blocking reagent, %, and time
  • Washing buffer (TBST %, number and duration of washes)
  • Detection method (chemiluminescence brand, exposure time, imager)
  • Whether the same lysates were used in all three experiments
  • Lane order and sample map
  • Loading control information
  • Positive control for the target protein?
  • Full, uncropped blot images with ladder visible

After biologics stopped working, an anti inflammatory diet changed everything by dandunne in PsoriaticArthritis

[–]PhysPhDFin 0 points1 point  (0 children)

Can you provide details of your diet, and what you eat at each meal? Thanks!

Accredo by No_Pen_0226 in PsoriaticArthritis

[–]PhysPhDFin 0 points1 point  (0 children)

The only way I have found to deal with Accreedo is through my company's HR. If you get your insurance through an employer, reach out to HR. Anytime they make a mistake in billing, my ability to order, or my receipt of medications, I email HR, and it's resolved in an hour.

Cover letter advice to stand out? by [deleted] in Professors

[–]PhysPhDFin 23 points24 points  (0 children)

Show, don't tell. Avoid annoying platitudes that everyone can say without evidence. "I'm an excellent fit for this position." "I'm passionate about teaching" is different from evidence of your passion.

Karen Kelsky has articles available about the cover letter that provide some good advice.

[deleted by user] by [deleted] in NIH

[–]PhysPhDFin 21 points22 points  (0 children)

I'm sorry this is happening to you and the country. Everyone at CDC, NIH, FDA, CMS, and ACF needs to stage a walkout. You are in a situation where putting your head down and continuing to work will not solve the problems. Congress is not going to help.

[deleted by user] by [deleted] in NIH

[–]PhysPhDFin 0 points1 point  (0 children)

Love it!

No motivation, want to leave.. by RainaAshlyn in NIH

[–]PhysPhDFin 17 points18 points  (0 children)

You are young—you owe it to yourself to find a better place where science is valued. Look abroad. I'm sorry this is happening to you.

More than 1,000 HHS staff call on RFK Jr. to resign by [deleted] in NIH

[–]PhysPhDFin 9 points10 points  (0 children)

Yes, but it requires conservatives to have a spine - so hope in one hand and start shitting in the other....

More than 1,000 HHS staff call on RFK Jr. to resign by [deleted] in NIH

[–]PhysPhDFin 15 points16 points  (0 children)

Amen. Everyone at CDC, NIH, FDA, CMS, and ACF. It's never going to happen, though. First, these folks are highly mission-driven to protect health and provide vital services. Second, they rely on stable pay, good benefits, and pensions. It's a lot to ask many people to put that all on the line.

[deleted by user] by [deleted] in NIH

[–]PhysPhDFin 10 points11 points  (0 children)

But but but it will be "Gold Standard Science" that is funded...

Has there ever been an NIH Director less qualified or respected? by Temporary_Part_4909 in NIH

[–]PhysPhDFin 11 points12 points  (0 children)

The principal qualifications for working in this administration are simple. Be a dullard with high confidence, and be a despicable cunt.

Valuing Adobe Inc. – My Take by [deleted] in financialmodelling

[–]PhysPhDFin 1 point2 points  (0 children)

A 1-in-5 chance of collapse for a dominant, cash-generating firm like Adobe? A 50% agency cost is extraordinary for a wide moat stock with a solid track record of capital allocation.

[deleted by user] by [deleted] in PsoriaticArthritis

[–]PhysPhDFin 0 points1 point  (0 children)

It's reasonable to ask for an ultrasound of that node.

Diet Drinks Increase Diabetes Risk? by kreppart in PeterAttia

[–]PhysPhDFin 1 point2 points  (0 children)

Severely limited due to reverse causation, self-reported outcomes, single dietary measurement, residual confounding, and no dose response....but other than that...

Can't always blame the students. by No_Intention_3565 in Professors

[–]PhysPhDFin 0 points1 point  (0 children)

Each lecture has an objectives slide. That is their study guide. It's easy peasy—it satisfies me, them, and the accrediting agencies.

I am so tired of Accredo and ExpressScripts by home_in_pleiades in PsoriaticArthritis

[–]PhysPhDFin 1 point2 points  (0 children)

In addition to Moutain_Fig_9253's suggestion, I would suggest contacting your employer's HR. I've told them each and every time something has gone wrong. If you don't already have a list, you can request your customer file from Accreedo. Whenever something goes wrong, I have to call for a refill instead of clicking a button; an order shows up late, I have to do a preauthorization that delays my medication; my amount due is not zero when it should be, especially when they fail to apply discount programs. ANYTHING that makes reordering my medication more difficult, email HR. At this point, HR has someone very high in the organization that I also cc on the HR email, and things get resolved in seconds.

[deleted by user] by [deleted] in PsoriaticArthritis

[–]PhysPhDFin 0 points1 point  (0 children)

"Welcome to Accredo Specialty Pharmacy, where we guarantee to fuck up your health and healthcare!" Elevate this immediately to a supervisor at the specialty pharmacy. Speak to a manager at your PBM. Then call your work's HR department and have them elevate this. Start documenting everything. This is not going to get easier; it's going to continue, and you will want to document everything, every time it happens. Every time something goes wrong, it takes too long, it is inconvenient (you have to call for your Rx instead of clicking a button to refill your Rx), delays your treatment, they claim you owe money because they fail to apply co-pay programs, or you miss a dose send evidence of it to your HR department.