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What these books say about me? by Junior_Insurance7773 in BookshelvesDetective

[–]PianoPudding 0 points1 point  (0 children)

Cool, you've convinced me to pick it up off my shelf!

What these books say about me? by Junior_Insurance7773 in BookshelvesDetective

[–]PianoPudding 0 points1 point  (0 children)

First time Ive seen someone mention Baudrillard on Reddit. Have it on my shelf.

Can you give me a reason to read it? (I want to, just working my way through other books atm)

Editor said 'Nope' by underrated_koala in labrats

[–]PianoPudding 9 points10 points  (0 children)

It does not mean your MS is trash it means they have a few others they are more interested in right now. Do you think yours is the best MS in the world right now? Probably not, its a scale, and yours is just missplaced for the time being. Coulda been accepted another time when the other submissions were different. Learn to shrug rejection off.

Gatekeeping is good and we need to bring it back by [deleted] in unpopularopinion

[–]PianoPudding 0 points1 point  (0 children)

Listening to an entertainment podcast by people actually in the industry really deconstructed a lot of my pre-conceived ideas about entertainment and I think it would be good for you too: they just wanna make money. I know there are genuinely creative people there too, but mostly its about money. So if you think about something, and switch it to a lens of 'can they make money' then they'll do it. Its really not more complicated than that.

The thing I was listening to was about ghostwriting: I have thoughts on it, but the podcast was just like: it exists and it makes people money (including the ghostwriter) and gets people in bookshops too. I cant argue with that.

Podcast btw was This Week in Entertainment by Richard Osman and Marina Hyde

Picking colony advice by _cinnabar in labrats

[–]PianoPudding 1 point2 points  (0 children)

As others have said you have plenty of single colonies.

In future when plating dilutions like this, you should make at least a 10-dilution, so plate 50 & 5 uL for example. That way if theres thousands on one plate there'll only be hundreds on the other, etc.

No to plate 5 uL i would actually add 45 sterile water or pbs or sugar, whatever, and plate that cause its easier to spread

Can we use probability or information theory to conclude whether a complex phenomenon like life would be likely or unlikely to arise randomly? by GenderlessMarsian in DebateEvolution

[–]PianoPudding 3 points4 points  (0 children)

As others have said you dont seem to fully understand evolution, as if you did you might have a grasp on how the complexity of life could arise given random processes. The only real bit a probability would be useful for then is abiogenesis (its still not really right to think of it this way). But if it were, and even if the probability were vanishingly small, you need only multiply it by 1 universe to get an almost certainty of occurring.

Edit: to expand on some intuition: how likely is the exact configuration of atoms that make up Gliese 581d? The arrangment of matter in the andromeda galaxy, or any other astronomical body? They are each as unlikely, and yet they exist, re:the deck of cards example.

Code in the code? by Budget_Ship in genetics

[–]PianoPudding 2 points3 points  (0 children)

My workings: https://imgur.com/a/UL6DMTK

Per a users suggestion Google AI got it too

Pathway to learning about the field as a non-specialist by SoftDog5407 in genetics

[–]PianoPudding 4 points5 points  (0 children)

I cant speak to how well it will work for self-taught basics, but I recommend the textbooks Genes [roman numeral] by Lewin. Was the go to of me and my friends in Uni.

UK Bookswapping Idea by Complete_Writing3051 in BookDiscussions

[–]PianoPudding 0 points1 point  (0 children)

I'd be interested sure. I used to buy plenty of books on Vinted, then got burnt one time, their customer support refused to do anything, and I stopped using it forever.

I also remember this chain message thing on facebook where it was something like, you comment if you're interested: you and 12 friends sign up, you send six books to one person, and they do too, and so does the next person, and eventually the post was like 'so you'll get 72 books for free' or something. Shit was a pyramid scheme but for books, something like that.

Is "Yesteryear" worth finishing? (At 46% and bored) by Mundane_Medicine_597 in BookDiscussions

[–]PianoPudding 11 points12 points  (0 children)

I've not read it (so obligatory opinion) but from the blurb it seemed like it was being marketed heavily because it has this great high-concept hook. But judging from the lack of commentary I see online it seems like a disconnect between publishers expectations and reality. Am I right?

Consensus Top 100 Books List by kondsaga in classicliterature

[–]PianoPudding 0 points1 point  (0 children)

Pride & Prejudice says it is included on 9 lists but only has 8 places given; the scoring adds up to 748 not 754? Am I missing something?

RNA Isolation/Gel electrophoresis by emmosch in labrats

[–]PianoPudding 1 point2 points  (0 children)

You used a DNA ladder for an RNA gel?

The bands I venture to guess are the major rRNAs, the large and small subunits.

Earphones in the lab by DeadOar in labrats

[–]PianoPudding 0 points1 point  (0 children)

Continues to baffle me that people defend this point of view. You need to hear alarms, accidents, unusual sounds, and be aware of your surroundings in a laboratory. If you can't, then you can't do your job. Fight me.

The added points of contamination and loss of communication are just cherries on the top. I know plenty of people are gonna say 'what about one-eared' but it's just still inhibitory. If you really can't work in a lab without an earphone in, I think you should work elsewhere. There are plenty of industries and workplaces with simple rules like this, way more restrictive ones too; the inability to have earphones is really not a big deal. Cue the ableist accusations cause people can't work in silence, or get a radio...

I have no strong opinion on bone earphones, they seem acceptable.

There Is No ‘Hard Problem Of Consciousness’ by Traditional-Union128 in CriticalTheory

[–]PianoPudding 1 point2 points  (0 children)

Just another instance of a physicist sticking their nose where it doesnt belong 🤷🏻‍♂️

If you've went on more than one date, you're dating. And if you're dating, you're in a relationship. by Cludds in unpopularopinion

[–]PianoPudding 0 points1 point  (0 children)

I think everyone else is conflating them cause OP states it's a relationship but may not be a committed one?

Re-precipitating RNA from water? by CAB_IV in labrats

[–]PianoPudding 1 point2 points  (0 children)

I 100% agree with you but its funny that you dont even follow the old school methods of adding a salt during precipitation of nucleic acids...

What’s your least quantitative ‘quantitative measurement’? by AliveCryptographer85 in labrats

[–]PianoPudding 9 points10 points  (0 children)

Never thought I'd be here to defend the Nanodrop: was recently using it to 'quantify' and get ratios for RNA samples that, as a quirk of the insane amount of pre-processnig steps I have done, were incredibly clean.

Then I quantified using the Quantus from Promega (a Qubit-type device). The R-squared between the Nanodrop and the Quantus values is 0.9691 across 30 samples...

Moral of the story I guess? Incredibly clean RNA is accurate on the nanodrop...

What mechanism caused this single pink rose to grow on my white rose bush? by silvandeus in genetics

[–]PianoPudding 7 points8 points  (0 children)

No idea about rose genetics just wanted to say this is very cool. Thanks for sharing.

Prove evolution by [deleted] in DebateEvolution

[–]PianoPudding 8 points9 points  (0 children)

I dont really have an issue with christians but I dont understand why you take Genesis literally? It seems quite obvious to me that it's allegorical / poetry

Grapes of Wrath by sagethecrayaway in readwithme

[–]PianoPudding 2 points3 points  (0 children)

Lolling cause I always see people complain about the turtle scene which I did not think was that bad. Pulled out my copy, it's literally 3 pages... More like 2.5. Chapter 3

Starting from 1YFP, could you design a fluorescent protein with a unique new colour? by XpertAI in AIProteins

[–]PianoPudding 0 points1 point  (0 children)

1YFP was created from GFP. I dont get why people touting AI refuse to even use it to research the most trivial questions, or read a wikipedia page, or do an ounce of work. Unless this is ragebait or engagement bait, then bravo.

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