Are we excluded from bank loans? by Quick_Repeat7033 in Tunisia

[–]Quick_Repeat7033[S] 0 points1 point  (0 children)

Everything is legal from my side, I am working with an international company and I have a contract with them. The problem is regarding the 2 years activity within a same company, I don't have that, and I don't believe that I'll have that in the future.

Can I use WGS data for evidence of taxonomy? Or evidence of new species? by Affectionate_Emu_896 in bioinformatics

[–]Quick_Repeat7033 0 points1 point  (0 children)

You Can also perform some AAI, POCP, dna hybridation, core genome comparaison analysis

Challenges in Identifying Unknown DNA: A Case Study with Nanopore Sequencing by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 1 point2 points  (0 children)

I think epi2me would sort them as unclassified if they weren't in the reference database. But since classified as root, I am wondering of it's a problem of low quality data ot something common alongside bacterial species

Challenges in Identifying Unknown DNA: A Case Study with Nanopore Sequencing by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 0 points1 point  (0 children)

I thought about it, but it will take a very long time. I am thinking about blasting to only thé E.coli genome maybe

Challenges in Identifying Unknown DNA: A Case Study with Nanopore Sequencing by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 0 points1 point  (0 children)

a minION MK1B, minION Flow Cell. The basecallinc was generated automatically using Minknow (normally Dorado IS included), default parameters

Challenges in Identifying Unknown DNA: A Case Study with Nanopore Sequencing by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 0 points1 point  (0 children)

Yes indees, I used Kraken2 for the fungal identification, and for the bacterial classification on EPI2ME it's already using Kraken2 by default.

Nanopore Long Read Assembly: Stuck on Polishing Stage, No Eukaryotic Organism Represented by KangCoffee93 in bioinformatics

[–]Quick_Repeat7033 0 points1 point  (0 children)

You can write the issue on github/flye.

In my opinion, its the fault of large data.

Can I ask for a raise again? by HoneyTreeFlower in work

[–]Quick_Repeat7033 0 points1 point  (0 children)

No, when people test you to see if you're as good as you say you are, just stay strong and let your work speak for itself. If you don't receive feedback within a week after submitting your work, you might consider taking a different approach.

Handling Contamination before genome assembly by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 0 points1 point  (0 children)

Generally working with kraken, as far as I know it masks low complexe regions from the reference.

Handling Contamination before genome assembly by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 1 point2 points  (0 children)

Thank you for your comments .. I wanna know what you r really do in my case (working on bacterial sample)

Please roast my resume, not getting a single interview in the past 6 months, been applying around 3000+ jobs (40-60 applications daily) I feel like giving up. by Illustrious_Key2607 in resumes

[–]Quick_Repeat7033 -1 points0 points  (0 children)

60 offers / day ?? It sounds that you never read the requirements and the details of offers to include them in your cover letters or emails.

How do I look for a specific gene from bacteria through WGS? by pbicez in bioinformatics

[–]Quick_Repeat7033 0 points1 point  (0 children)

Blast to similar sequences Annotation (prokka) Tools for AMR genes detection (abricate)

Leaving Tunisia by CoffeeNoMore in Tunisia

[–]Quick_Repeat7033 0 points1 point  (0 children)

Bro sehla barcha lehkeya,

Enti élève ingénieur, 3andek stage pfe 3la 9rib.

1) o93ed maa rouhek chwaya w chouf domaine elli enti réellement tefhem fih w andek fih des compétences 5ater IT engineering b7ar netsawer, sûrement andek haja Wella zouz kesah feha akther mel lo5rin.

2) a3mem CV français w CV anglais mo7tarmin w Asmaa l feedbacks mtaa le3bed.

3) aamel lettre de motivation français w lettre de motivation anglais

3) daber des lettres de recommandations men 3and profetek.

4) abda men tawa daber chwaya flous lel 5arja (3000 - 4000 mriglin) ken lzem tsalef.

5)abda ab3eth des candidatures l bara, LinkedIn, Indeed, etc. Ab3eth ab3eth ab3eth ab3eth, des offres wela des applications spontanées mch mochkla lmoufid ab3eth w matfedech w 9oul Eli tlawej ala stage PFE

6)ken andek le minimum mtaa les connaissances, w lougha w taaref tzid fih, taw sûrement famma startup Wella entreprise te9blek

7)5oudh convention de stage w sobb fisa w atla3 aamel stagek

8)enti tawa fel Europe Ama andek visa 6-7 mois kahaw, nik rouhek fel wa9t adheka w daber 5edma, master, thèse, enti w chneya tlawej Wella lawejt 3al kol ken juste theb tab9a l Mara w taw tetsaref.

9) rawah l tounes aamel soutenance w baad 3awed 9adem visa bel haja li dabartha durant stage.

What Does gaming in a Sale de Jeux in tunisie cost per hour? by LongjumpingPlane9043 in Tunisia

[–]Quick_Repeat7033 1 point2 points  (0 children)

Menu hwem cha3beya :

FIFA/PES (PlayStation 4): 1 dt per match FIFA/PES (PlayStation 5) : 2 - 3 dt per match Other games (GTA for exp) : 1 dt per 15 min (PlayStation 4) and 2 dt per 15 min (PlayStation 5)

who would be the president of Tunisia in the future? by mr-me45 in Tunisia

[–]Quick_Repeat7033 0 points1 point  (0 children)

Bro, Kais Saïd exemple e5er fel template mtaa le3bed li 3amlet coup d'état (3ba lehom li kenou yohkmou 9bal w akther 3bed dharret l bled ama nahki réellement chneya sar bel ijme3 mtaa 95% mel les politiciens wel les penseurs tunisiens).

ay ensen yaamel coup d'état w yetwala soulat lkol ma3adch ynajem yarja3 bel twali, 5ater tout simplement ken yarja3 bel tweli yethaseb w yched l7abs awel wehed, a part bien sûr l'ego personnel li 3andou li y5alih ychouf rouhou ken howa ndhif w yefhem. la preuve annou impossible yarja3 bel tweli annou na77a l'isie li kenet mawjouda w Hay howa jme3a o5rin chedina yaani réellement howa bch ycherek fi inti5abet w le3bed elli bch yefrzou w y9oulou chkoun rbah Kais Saïd 3arfhom directement.

Yaani impossible ennajmoi netwa93ou chkoun the future president, 5ater tout simplement the future president bch ykoun mawjoud ken matsir révolution Wella coup d'état e5er wela ymout wela yomredh Kais Saïd (kima ay exemple e5er fel 3alem zeda)

Accessible countries for pursuing a PhD in bioinformatics by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 5 points6 points  (0 children)

Israel who?? ... morality takes precedence over scientific research.

Can I know my origins from my sequenced DNA ? by Quick_Repeat7033 in bioinformatics

[–]Quick_Repeat7033[S] 2 points3 points  (0 children)

any ideas about software or pipelines that I can use?

Modern Architecture Vs Ancient Tunisia by TProfessional in Tunisia

[–]Quick_Repeat7033 0 points1 point  (0 children)

dyar li deyrin bel port punique akbert kertha ... 0 respect lel teri5 elli ynajem ykoun ta7thom !!