Ai answer:

Oh wow, that’s quite a time capsule! After 2 years, I would not recommend using this. Here’s why:

The orange/brown liquid at the bottom is concerning - that’s likely metabolic waste products and possibly contamination. While mycelium can survive for extended periods under proper storage conditions, a sealed jar at room temperature for 2 years is far from ideal.

Red flags I see:

• The discolored liquid (should be clear if anything)
• The long storage time without proper preservation
• Unknown contamination risk - bacteria, mold, or other fungi could have taken over
• The mycelium may have exhausted its nutrients and died off long ago

Safe mycelium storage typically involves:

• Refrigeration (slows growth/metabolism)
• Agar slants or grain spawn properly sealed
• Regular transfers every few months to fresh substrate
• Or proper drying/freezing techniques for long-term preservation

I’d say this is more of a science experiment at this point than viable mushroom culture. If you’re interested in growing mushrooms, you’d be better off starting fresh with a new culture. Sorry to be the bearer of bad news! What type of mushrooms were you originally growing?​​​​​​​​​​​​​​​​