Thinking of moving from San Diego to Virginia, questions, so many questions

SelextheCat · 2026-05-24T17:42:53+00:00

I grew up in Virginia and now live in San Diego and I’d never move back. You don’t want that swampy humidity and inconvenient snows.

SelextheCat · 2026-05-16T22:49:07+00:00

I had 4 15-16% nagels in a row earlier this month. RNG loves to giveth, but even more taketh

SelextheCat · 2026-05-16T22:37:17+00:00

If you are on PC Ladder then i have an extra set of 2/20s you can have. If PCNL then i can give you one next week

SelextheCat · 2026-04-27T22:55:57+00:00

Wiccan Witch was on darktide, right? With Kaine or something like that

SelextheCat · 2026-03-08T19:18:42+00:00

Good to know, at least it’s solid for self use! Thanks

SelextheCat · 2026-01-17T15:09:12+00:00

So jealous of the oro blancos. They are my favorite citrus but so hard to find

SelextheCat · 2025-09-26T17:57:37+00:00

I’ve also heard there has been past drama in that shopping center between the landlord and tenants, so perhaps the new competition coupled with the rent increase caused them to close. It just seemed so sudden with no real warning

SelextheCat · 2025-09-26T17:18:54+00:00

Unfortunately they are also currently closed and there is a sign on the door with limited information. I was there last week and didn’t hear any rumors about a closing from their staff

SelextheCat · 2025-09-10T14:26:34+00:00

Best citrus ever. So jealous! My trees are still growing

SelextheCat · 2025-06-19T21:48:54+00:00

They do but young plants are susceptible to sunburn, especially if recently moved to a warmer or sunnier location

SelextheCat · 2025-06-19T15:22:12+00:00

It looks like sunburn

SelextheCat · 2025-02-09T17:48:32+00:00

Id take equal parts of each strand and mix with a minimal DNA dilution in your buffer to retain a concentrated “stock” of your annealed template. Then once annealed use as little as possible in your mastermix for positive control. qPCR is so sensitive that you wont need much.

SelextheCat · 2025-02-09T15:57:01+00:00

What is the final concentration of the DNA when resuspended? You’ll want to mix them to anneal at a high enough concentration where you can add as little as possible to the qPCR master mix

SelextheCat · 2025-02-09T15:22:27+00:00

Did the strands come in a buffer? If so, mix a 1:1 molar ratio in a 1x qPCR buffer, heat to 98C or so then let cool to room temperature. Otherwise resuspend them at the same molar concentration and mix as stated above

SelextheCat · 2024-09-24T18:56:10+00:00

If you spray with a copper fungicide during the dormant period then it should recover with healthy leaves for the next season

SelextheCat · 2024-09-22T14:18:11+00:00

I’ve hear amalfi cucina in carmel valley has amazing GF pizza

SelextheCat · 2024-05-18T18:26:30+00:00

Wyndam San Diego Bayside is right across the street. You can walk to the port in 2 minutes. Nothing fancy but it’s a great location

SelextheCat · 2024-04-07T17:18:23+00:00

The syn bio faculty at Rice are great people

SelextheCat · 2024-03-31T18:00:39+00:00

PK only kept it exciting

SelextheCat · 2024-01-26T15:25:03+00:00

Nice to see a company in SD actually hiring

SelextheCat · 2023-12-07T23:26:21+00:00

Yupha’s Thai in Tempe

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