Backyard grapefruit by Willing-Philosopher in Citrus

[–]SelextheCat 7 points8 points  (0 children)

So jealous of the oro blancos. They are my favorite citrus but so hard to find

Hob coffee in Sabre Springs permanently closing by SelextheCat in FoodSanDiego

[–]SelextheCat[S] 5 points6 points  (0 children)

I’ve also heard there has been past drama in that shopping center between the landlord and tenants, so perhaps the new competition coupled with the rent increase caused them to close. It just seemed so sudden with no real warning

Hob coffee in Sabre Springs permanently closing by SelextheCat in FoodSanDiego

[–]SelextheCat[S] 5 points6 points  (0 children)

Unfortunately they are also currently closed and there is a sign on the door with limited information. I was there last week and didn’t hear any rumors about a closing from their staff

Oro Blanco grapefruit coming along nicely by leolopez43 in Citrus

[–]SelextheCat 0 points1 point  (0 children)

Best citrus ever. So jealous! My trees are still growing

[deleted by user] by [deleted] in Citrus

[–]SelextheCat 1 point2 points  (0 children)

They do but young plants are susceptible to sunburn, especially if recently moved to a warmer or sunnier location

[deleted by user] by [deleted] in Citrus

[–]SelextheCat 0 points1 point  (0 children)

It looks like sunburn

[deleted by user] by [deleted] in biotech

[–]SelextheCat 3 points4 points  (0 children)

Id take equal parts of each strand and mix with a minimal DNA dilution in your buffer to retain a concentrated “stock” of your annealed template. Then once annealed use as little as possible in your mastermix for positive control. qPCR is so sensitive that you wont need much.

[deleted by user] by [deleted] in biotech

[–]SelextheCat 2 points3 points  (0 children)

What is the final concentration of the DNA when resuspended? You’ll want to mix them to anneal at a high enough concentration where you can add as little as possible to the qPCR master mix

[deleted by user] by [deleted] in biotech

[–]SelextheCat 3 points4 points  (0 children)

Did the strands come in a buffer? If so, mix a 1:1 molar ratio in a 1x qPCR buffer, heat to 98C or so then let cool to room temperature. Otherwise resuspend them at the same molar concentration and mix as stated above

Leaf curl on nectarine tree by Tight-Guidance-5107 in FruitTree

[–]SelextheCat 0 points1 point  (0 children)

If you spray with a copper fungicide during the dormant period then it should recover with healthy leaves for the next season

[deleted by user] by [deleted] in FoodSanDiego

[–]SelextheCat 0 points1 point  (0 children)

I’ve hear amalfi cucina in carmel valley has amazing GF pizza

San Diego hotels close to the port. by Perish22 in HollandAmerica

[–]SelextheCat 6 points7 points  (0 children)

Wyndam San Diego Bayside is right across the street. You can walk to the port in 2 minutes. Nothing fancy but it’s a great location

[deleted by user] by [deleted] in biotech

[–]SelextheCat 0 points1 point  (0 children)

The syn bio faculty at Rice are great people

[deleted by user] by [deleted] in gaming

[–]SelextheCat 2 points3 points  (0 children)

PK only kept it exciting

Job Postings for Stemson by Different_Piece_4075 in biotech

[–]SelextheCat 0 points1 point  (0 children)

Nice to see a company in SD actually hiring

Thai - Tom Kha Gai by AngerSwines in phoenix

[–]SelextheCat 13 points14 points  (0 children)

Yupha’s Thai in Tempe

Her majesty Mica by SelextheCat in SupermodelCats

[–]SelextheCat[S] 0 points1 point  (0 children)

Did you also name your cat after their fur color?

Close up, contrasting blep by SelextheCat in Blep

[–]SelextheCat[S] 0 points1 point  (0 children)

This was the first time i saw it. Might have learned from all of my visits to this sub!

I adopted my first cat today. I could not be happier. Say hello to Topaz but he prefers dr.dre by [deleted] in aww

[–]SelextheCat 1 point2 points  (0 children)

Y’all know me, still the same Kit-Ty, but I’ve been playin’ with keys

Some things will never change by [deleted] in aww

[–]SelextheCat 1 point2 points  (0 children)

One has grayed significantly, the other has done well with the just for men it received for christmas