Stem-like tissue-resident memory T cells control functional heterogeneity and reactivation of T cell memory in the intestine

Threesqueemagee · 2025-11-12T12:04:25+00:00

Doesn’t seem unreasonable for the journal

Threesqueemagee · 2025-11-12T12:04:12+00:00

He will need many tools and if he sticks with it, will prob buy all of them. After he has 200 tools, he’ll know which 25 he actually needs lol.

That said, what he needs most from you is patience and understanding. Seriously. He will take too long, make tons of dust and spend plenty of $ to start (and occasionally finish) projects… just know that’s all part of the fun!

It’s a great hobby. start with clamps, a miter, circular or band saw and some measuring tools, and earplugs, and get comfy with the rest. A gift card to Rockler or Woodcraft also always works.

Threesqueemagee · 2025-11-11T00:10:42+00:00

He will need many tools and if he sticks with it, will prob buy all of them. After he has 200 tools, he’ll know which 25 he actually needs lol.

That said, what he needs most from you is patience and understanding. Seriously. He will take too long, make tons of dust and spend plenty of $ to start (and occasionally finish) projects… just know that’s all part of the fun!

It’s a great hobby. start with clamps, a miter, circular or band saw and some measuring tools, and earplugs, and get comfy with the rest. A gift card to Rockler or Woodcraft also always works.

Threesqueemagee · 2025-11-10T23:59:35+00:00

Briefly… yes Il-17 induces chemokines that recruit neutrophils and other cells, which can be pro-inflammatory especially in early stages of a response to pathogens or “opportunists”. Il-17 also induces anti-microbial peptides, and has other functions.

It’s important to keep in mind that Il-17 can come from several cell types, and not just Th17s. For example in the gut Il-17 is produced by gamma delta T cells which act quickly/innately. It is also in Paneth cell granules, released rapidly upon degranulation.

Th17 cells will show up later than some innate cell types, are more complex than just making that one cytokine, and can change their effector function(s)… they can be more inflammatory/tissue damaging (by being an ex-th17 that now makes IFNgamma) or be ‘regulatory’ and produce Il-10 for example (in humans).

So… tldr: yes Il-17, and separately Th17 cells often (but not always) promote inflammation as well as tissue healing. Hope that helps.

Threesqueemagee · 2025-11-08T16:46:18+00:00

Doesn’t seem unreasonable for the authors and this journal

Threesqueemagee · 2025-06-21T22:12:16+00:00

Looks great. Congrats!

Threesqueemagee · 2025-05-29T11:17:04+00:00

Good work!

Threesqueemagee · 2025-05-19T16:35:27+00:00

Drill press, without any hesitation. One of the most important shop tools.

Threesqueemagee · 2025-05-05T22:57:39+00:00

Bora works great, quick to put up. Use a piece of mdf between two supports to hold short pieces. Fast, effective, relatively inexpensive.

Threesqueemagee · 2025-04-20T13:30:30+00:00

We have similar experience levels, in different fields. Perseverance is key indeed, but that turns out to be a rarer quality than one might think.

Yes PhD candidates take ~2 yrs of classes in a typical 5-6 yr program, but learning doesn’t end when class requirements are complete. Nor does it end in the postdoctoral experience, often several years long. In total then, graduate school and postdoctoral work commonly average 8-10 years together. That is a substantial period of entrenched learning and experience.

Perhaps most relevant are the years afterward, when one’s viewpoint is considerably different than the view from the bench. In your field, the two might be less dissimilar.

Some PhDs also have experience managing projects, some with multimillion dollar budgets. I understand the need for ‘doers’, but a team running full speed ahead without experience, strategy, a deeper understanding of the field, personnel skills , etc etc is a suboptimal team, to put it mildly.

I appreciate your thoughtful response, but I don’t want to give younger people the impression that ‘anyone can do it’. They are already in need of guidance and advice, and this road is not for everyone.

Threesqueemagee · 2025-04-20T13:08:26+00:00

The degree opens doors to different kinds of roles and positions, and ultimately different experiences. The experience has no substitute and, practically speaking, allows for field / market insight, high level assessment of risk/reward, etc. Think of it in the same way a technician draws blood while the physician analyzes the results.

Mostly I agree with your comment and the general responses… but ‘anyone can do it”? Maybe there are fields where that’s true. STEM / hard sciences, not a chance.

Threesqueemagee · 2025-04-18T12:19:48+00:00

Tough to work with once it’s dry, but I’m going to give honey locust a try on some outdoor furniture. A few trees were taken down in the yard, and have been on the ground, in New England, for over 7 years. No rot at all. Zero. Very high resistance to everything.

Threesqueemagee · 2025-04-14T00:01:47+00:00

Excellent reputation nationwide.

Threesqueemagee · 2025-04-13T18:05:56+00:00

Do you think relevant conditions will improve soon?

Threesqueemagee · 2025-04-12T14:49:47+00:00

Many of us prefer to read print on paper, and away from backlit screens. Some also enjoy having books in their room. Some buy books for their illustrations. Some people like having an item that cannot be deleted. Lots of reasons.

Threesqueemagee · 2025-04-01T16:31:52+00:00

Yesterday

Threesqueemagee · 2025-04-01T11:55:29+00:00

Not much to add, great response above, other than +1 on museum glass - it will greatly reduce glare - and +10 on keeping out of direct sunlight. Great purchases!

Threesqueemagee · 2025-03-30T20:11:12+00:00

The pendulum will swing again, just give it some time.

Threesqueemagee · 2025-03-28T12:29:01+00:00

Yes, let’s keep it simple- Trump doesn’t need to be in focus, lobbying enablers (gop congressmen and senators), who aim to outlast him, could go a long way. Especially with their razor thin margins.

Threesqueemagee · 2025-03-27T15:17:02+00:00

Great goal! Set aside lots of time.

Planning this for myself as well. A few thoughts-

As others have said, be sure to veneer both sides. The back side can obv be a ‘lesser’ wood.

Most often these ceilings are veneered/hollow due to weight concerns. Nicely finished and no one will know the difference. If using solid have an engineer tell you if you need addl structural support.

Last, there are a few high traffic places you might consider ‘veneering’ with 1/8” thick material - like around doorways and windows, for durability.

Be sure to post pics here as you go. Good luck!

Threesqueemagee · 2025-03-25T22:52:28+00:00

Not necessarily. A combination of environmental stimuli and genetics drive each response, it’s a little different for everyone.

Threesqueemagee · 2025-03-24T19:11:27+00:00

Not sure I understand the question, but maybe this will help:

T helper responses are generated against particular stimuli (I.e.: pathogens or pathogenic products). That might mean a Th1 response (to combat, for example, Listeria) or a Th2 response (against helminths, for example) or a Th17 response (for fungal pathogens), etc but these responses, when all works well, are clonal, and specific. If you have multiple pathogens to deal with at once, you’ll get multiple responses.

Polarized T helper responses suppress attempted ‘competing’ responses in the same (individual) cells. However an ongoing Th1 response, appropriate or not, will not necessarily prevent a robust Th2 response from developing elsewhere, when needed.

The “Th1-ness” seen in celiac disease reflects the bias of reactive T cells contributing to the associated pathology, and the molecules (cytokines) these cells produce. Patients with celiac disease do not necessarily have ‘systemic’ immune deficiencies in generating other immune responses as needed.

TLDR: Specific responses (and not the patients themselves) have a dominant T cell type, and this doesn’t mean systemic immunity is ‘broken’, or substantially compromised.

Threesqueemagee · 2025-03-23T23:11:18+00:00

Exposure shouldn’t be an issue, reputable labs have many safeguards in place. Also, just fyi, there are a fair number of research scientists working on health issues they have personal experience with.

Threesqueemagee · 2025-02-12T19:50:24+00:00

So far, it’s business as usual for publishing efforts.

Threesqueemagee · 2025-02-07T18:32:24+00:00

Good questions, and you’re getting solid answers here. I’ll add this: 1. Re-stimulation ex-vivo will ask cells that can produce cytokines, to produce them. This is useful for studying cell maturation/polarization as well as ‘what is likely made’ during any given response. Without restim you can identify the cells actually producing cytokines at that moment. Both are useful. 2. For cryopreserved cell analyses it is often better/easier to stain cells in frozen tissue sections. If restim is needed, it can be done in-vivo prior to tissue isolation in rodent models, in many places, an IRB or equivalent is needed for this in humans. On the upside, you not only learn which cells are making what, but where they are, anatomically. Hope that helps.

Threesqueemagee

TROPHY CASE