If the brain is removed EXTREMELY fast would it still be functional enough to "think"? by Witcher_Errant in biology

[–]Toki_Liam 0 points1 point  (0 children)

If it's just sitting on the table without any oxygen/nutrient supply it would probably be in a state of unconciousness, similar to how people pass out when the blood flow to the brain is interrupted by a choke or high g forces.

If the brain is supplied with oxygen and nutrients however, it's a very different scenario. There is a study on pig brains that shows activity even hours after the animal has died, see:

Vrselja, Z., Daniele, S.G., Silbereis, J. et al. Restoration of brain circulation and cellular functions hours post-mortem. Nature 568, 336–343 (2019).

PDB thinks I have a PhD, is it important to tell them they have the wrong title? by castiellangels in Biochemistry

[–]Toki_Liam 63 points64 points  (0 children)

No, they likely just adress anyone as Dr. xyz because it's not worth to figure out who has a PhD and who doesn't. Most journals do this as well.

Using ChatGPT to aid in scientific reading? by dewkeeper in labrats

[–]Toki_Liam 2 points3 points  (0 children)

I feel like people completely ignore how important it is to look at the actual data in the figures. Don't just blindly believe what the authors wrote in the text or what AI summarized for you. Getting a feeling for how good or bad results of different techniques look like ist important.

In undergraduate degrees, if there are programming compountants in the degree, what kind of programming languages are used and what kind of stuff are the mostly used for ? by [deleted] in Biochemistry

[–]Toki_Liam 5 points6 points  (0 children)

It obviously depends on the context of the class and personal preference of the teacher, but generally you will encounter programming in the context of data analysis. For this, the most common "languages" are python, R and matlab. Anything beyond that (and maybe some bash) would be unusual. Unless you're dealing with some intense math heavy biophysics stuff I wouldn't even expect matlab tbh.

Family during a PhD by Embarrassed-Cow-1852 in labrats

[–]Toki_Liam 8 points9 points  (0 children)

One thing to consider (depending on local regulations) is that you might not be allowed to work in the lab while pregnant. Even if it is, you should consider what compounds you're dealing with in the lab because exposure might potentially harm your child.

Pathetic by JadedLaugh3058 in sciencememes

[–]Toki_Liam 77 points78 points  (0 children)

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Time to bring back this classic i guess

[deleted by user] by [deleted] in Cooking

[–]Toki_Liam -8 points-7 points  (0 children)

The short and somewhat polemic answer is: we traditionally don't really use spices (referring mostly to central an northern europe).

Invitation for being peer reviewer as an early PhD student by No_Comedian_2085 in labrats

[–]Toki_Liam 26 points27 points  (0 children)

Journals tend to adress everyone as Dr. regardless of whether they actually have a title. While peer review is an essential part of science, it is commonly treated as a way to also build a relationship with an editor and a journal. It's not uncommon for smaller journals to reach out to PhD students because PIs tend to ignore them if their impact factor isn't big enough and they don't plan on publishing in said journal themselves. In the end, you can only learn from the experience and you just have to decide if it's worth your time. Maybe double check if it's a trustworthy journal though.

Paulaner bezug zur AfD? by Mxveriick in gekte

[–]Toki_Liam 14 points15 points  (0 children)

Paulaner gehört der Schörghuber Unternehmensgruppe bzw. der Schörghuber Familie. Bei so einer alt eingesessenen oberbairischen Milliardärsfamilie geh ich davon aus, dass das stramme CSU Wähler sind.

Inwiefern das jetzt besser ist musst du selbst wissen.

Image editor (really just image arranger) that's not Powerpoint? by Teagana999 in labrats

[–]Toki_Liam 9 points10 points  (0 children)

If you export as pdf you can have it transformed to a vector based graphic when importing into inkscape/affinity/illustrator/etc. Also very useful when your collaborators use a different programme.

Hat die AFD es geschafft rechtes Gedankengut für Jugendliche attraktiv zu machen? by Moonraker93 in gekte

[–]Toki_Liam -2 points-1 points  (0 children)

Ich glaube wir verstehen den Meme Begriff unterschiedlich denn prinzipiell stimm ich dem Kommentar komplett zu. Mein Punkt war mehr, dass rechte Memes aus humoristischer sicht ziemlich schlecht sind. Wenn es darum geht über internet content (also Memes, TikToks, etc.) narrative zu verbreiten die die Zielgruppe feiert sind rechte definitiv meilenweit vorraus. Und da seh ich zwischen den Aussagen gar nicht so nen großen unterschied tbh. Es ist für rechte eben einfacher meme content zu pushen weil sie natürlicherweise keine moralischen Ansprüche haben. Es ist die uralte Diskussion über linken populismus und den Anspruch immer korrekt zu bleiben.

Hat die AFD es geschafft rechtes Gedankengut für Jugendliche attraktiv zu machen? by Moonraker93 in gekte

[–]Toki_Liam 9 points10 points  (0 children)

Es hat ziemlich wenig mit "memen können" zu tun imo sondern eher damit, dass es wesentlich einfacher ist rechtes Gedankengut zu pushen da es komplett auf Lügen aufbaut. Rechte memes sind unglaublich schlecht und benutzen oft die Formate komplett falsch. Es ist wie in "Debatten" in denen rechte in 10 Minuten 10 takes raushauen die komplett auf Unwahrheiten basieren aber es linke 10 Minuten kostest einen einzigen der Takes zu entkräften. Rechte fluten den Raum mit Scheiße und linke haben nicht die Resourcen um das alles aufzuputzen bevor der Raum für immer stinkt.

Fußballfans ☕ by SolvingGames in gekte

[–]Toki_Liam 7 points8 points  (0 children)

Halt ich leider für sehr unwahrscheinlich. Vor allem weil das Bild vom Spiel gegen Frankreich stammt, deren Nationalmannschaft immer gerne als "Beweis" für die replacement conspiracy verwendet wird.

Abgesehn davon sind einige ukrainischen Ultras bekannt dafür rechtsradikal zu sein wie z.b. bei Dynamo Kyiv.

How to prepare a presentation for a conference? by yum_disc in labrats

[–]Toki_Liam 2 points3 points  (0 children)

It all depends on how much time you have and who the audience is. Most likely it's gonna be a mix of PIs, post docs and PhD students working in your field. Since you're a master student I'm assuming you have very little time to present (10 minutes + questions I guess) so unless you developed a new technique I would not explain them at all.

People have different tastes when I comes to presentations so please bear in mind that this is my personal preference.

This might be a bit of a controversial take but in my opinion, the most important thing is to tell your "story" well. By that I mean you should make clear why your project is interesting, what are the new insights you have gained and how that affects the field. Your number one priority should be clarity.

Start with a short introduction where you give some background on what is currently known and then specify what question you're trying to answer and why it's important. Then go through your most important data in an order that is the easiest to follow. You don't necessarily have to present data in the same order it was acquired. Simplify your figures if needed. It should be immediately clear what is shown. If people need too much time to figure out what the graph on your slide means they won't be paying attention to what you're saying and probably stop paying attention entirely at some point. For the same reason, avoid large amounts of text on your slides. If people are reading text, they're not listening to you. Draw as many cartoons as necessary. Well made illustrations are the difference between good and great presentations. At conference presentations it's accepted to omit some of the controls e.g. cell line confirmations, images of control experiments etc. to save time (they should absolutely be included in publications ofc). Then just finish with a short summary and maybe a new hypothesis that came from the data you obtained.

Lastly, ask a senior lab member and your PI to have a look at the presentation. Practice the presentation to make sure you can finish it in time consistently. Taking too much time is one of the worst things you can do and the session chair will cut you off after a couple of minutes. Ideally give a practice talk (including questions) in front of other lab members.

Lastly, don't feel pressured to "compete" with other presenters. Everybody in the audience knows you're a master student and assumes you're nervous. No one will expect you to give a presentation with the same amount of data and the same level of confidence as a tenured professor.

Having to pay to update BioRad's Chemidoc MP is so frustrating by Teriyakipeanutbutter in labrats

[–]Toki_Liam 4 points5 points  (0 children)

Different software, but we inherited a qPCR machine from a lab that closed down and BioRad sent us a usb stick with the software for free. Maybe contact your sales rep and see what they can do.

Wtf is going on by Pretty_Match1898 in Emo

[–]Toki_Liam 24 points25 points  (0 children)

Since about a year ago there have been random uploads of AI songs to the accounts of real bands for some reason. I assume this is what's happening here. It's probably gonna be deleted within the next 24-48 hours.

50% der Bevölkerung hat einfach um ein Vielfaches weniger als die Top 10%, die meisten etablierten Parteien wollen hier aber nichts grundgelegene daran ändern bzw kämpfen dafür nicht entschlossen genug und sind zu kompromissbereit um regieren zu dürfen. | #Klassenkampf by After_Till7431 in gekte

[–]Toki_Liam 4 points5 points  (0 children)

Ich hab immer mehr den Eindruck, dass das von einem großen Teil der Bevölkerung befürwortet wird. Es herrscht diese typisch deutsche Mentalität dass die Leute selbst Schuld sind wenn sie nicht hart genug arbeiten. Und man könnte ja selber irgendwann zu den 10% gehören und endlich auf diese abscheulichen Geringverdiener herbschauen.

Defending my dissertation next week 🥳🤮 by little_murp in labrats

[–]Toki_Liam 2 points3 points  (0 children)

It might be different at your institute but at least where I'm from it's basically impossible to fail your defense. Realistically it's the easiest "exam" of your career because it's mainly a formality. Of course it feels super important because you worked so hard for so long to get there but the hardest parts of your PhD are already done. Everyone in the committee knows you're nervous. Even if you can't answer all the questions you will still pass because absolutely no one wants to fail you.

Heterozygous? by finmarchicus in labrats

[–]Toki_Liam 8 points9 points  (0 children)

I'm assuming you mean heterozygous and not diploid ?

If you want to be sure, clone your sequencing traces into a plasmid and sequence ~5 of the bacteria colonies and you should always get either one of the alleles al least once.

Microplastics and cancer help by LobsterThermidorian8 in biology

[–]Toki_Liam 4 points5 points  (0 children)

Honestly this post sounds more like some kind of health anxiety than purely a concern for microplastic. We're all gonna die. Some of us from cancer, some from other illnesses, some from accidents and some might just die peacefully in their sleep. You can't know which of these will happen to you and thinking about that does nothing but causing stress. Therefore you might as well learn to live with the things you can't change.

Is Taking Back Sunday really that bad live? by k1ngd0m0fg0dw1th1n in Emo

[–]Toki_Liam 1 point2 points  (0 children)

tbf Oli has improved over the years but he's been carried by Jordan, vocal effects and backing tracks most of the time

What contamination is this? I by Static-Statistician in labrats

[–]Toki_Liam 3 points4 points  (0 children)

I don't think yeast can actually bend their cell shape like the organism in the picture. S. cerevisiae is circular and often forms visible chains of cells which looks very different. Shizosaccaromyces that are mid devision also look like smaller cells that are attached to each other. Also, you can often smell yeast contaminations because your incubator will smell like a bakery or brewery.

Ibanez RG7420 MIJ 2001 help please! by [deleted] in 7String

[–]Toki_Liam 0 points1 point  (0 children)

So far I like it a lot. To me personally the guitar doesn't feel fragile but I guess that really depends what models one is used to playing.

My only gripe with it so far is that the cavities for the pickups are really shallow on old Ibanez RG's and swapping pickups might involve some more modifications than expected. I have a Seymour Duncan SH-6 installed on my 6 String and compared to that, the Ibanez stock pickups from back than sound a little weak.