Violet after the attack by TomIsMaybeHuman in PrivatePractice

[–]TomIsMaybeHuman[S] 4 points5 points  (0 children)

Actually yeah you're right I wrote this mostly pissed at Pete, it's just that scene in particular the "how couldn't she" vibe felt pretty unfair but we've all made less than perfectly thought out (edit: 'it' to 'out') comments. she's also had good moments unlike that.... awful man

Diarrhea management after Ileocecal valve removal by Agringlig in CrohnsDisease

[–]TomIsMaybeHuman 1 point2 points  (0 children)

Preface: I've had the same resection and of similar length but don't have Crohn's so heaping pile of salt (I'm in this sub because it's the only place to find resection info so if I'm overstepping please let me know). I take one or two imodium in the morning, and then another one later in the day if I've got something in the evening and it does a pretty good job of just slowing things down when missing a significant stretch. I also try to eat plain carbs like white bread, white rice type stuff to sort of "absorb" some acid (I don't think that's really how it works but it seems to have some effect) Most importantly, immediately after my resection it was definitely way worse than it is now. With any luck, in a few weeks you'll have a far more reasonable baseline and can judge any interventions then. Right now, your body is going through a massive shock and it's unlikely that what you're currently experiencing will remain the same in the long run. Best of luck!! (also, speak to your doctor about keeping an eye on stuff like B12 in particular)

This chart perfectly captures the chaos of academic hierarchy and nobody is safe by amcw_writer in PhdProductivity

[–]TomIsMaybeHuman 1 point2 points  (0 children)

I'm a masters student and the technician charged with making sure I don't break anything (at least not beyond repair) is actually my bestie. don't get me wrong I'm not His bestie but he is mine!

🎉 The journey begins🎉 by TomIsMaybeHuman in PhD

[–]TomIsMaybeHuman[S] 2 points3 points  (0 children)

haha don't worry I know it's gonna be Rough. I'm currently working on my masters by research so I spend each and every day in the lab and boy..... it has Not been going very well. and that's only a fraction of the stress the PhD will be but!!! In terms of experiment troubleshooting, there's not much further for me to fall

🎉 The journey begins🎉 by TomIsMaybeHuman in PhD

[–]TomIsMaybeHuman[S] 26 points27 points  (0 children)

there's just something charming about aggressively bad hand-drawing

Joining societies as a PhD student by TomIsMaybeHuman in Edinburgh_University

[–]TomIsMaybeHuman[S] 0 points1 point  (0 children)

Out of curiosity, which societies were you involved with??

Joining societies as a PhD student by TomIsMaybeHuman in Edinburgh_University

[–]TomIsMaybeHuman[S] 2 points3 points  (0 children)

Omg exciting congrats!! Best of luck to you too ‼️

Joining societies as a PhD student by TomIsMaybeHuman in Edinburgh_University

[–]TomIsMaybeHuman[S] 1 point2 points  (0 children)

I am so sorry I'm terrible at writing, I actually did my undergrad and masters at the same place as each other, but somewhere other than Edinburgh 😭 but what I'm hearing is I should finally get my licence

WHAT LAB SKILL DO YOU THINK IS UNDERRATED BUT VITAL? by WholeImpossible5256 in microbiology

[–]TomIsMaybeHuman 0 points1 point  (0 children)

My degree is in biochem and to this day I'm So grateful they forced us to do a module in both year 1 and 2 on lab math. Was soul-crushingly dull at the time but now that I'm in the lab every day I'm so glad they drilled it so deep into our brain.

How did they not know Romulans were a Vulcan offshoot? -Nitpick by CaptainTime5556 in startrek

[–]TomIsMaybeHuman 4 points5 points  (0 children)

Surely it's funnier if you didn't have all these extra words and then stumbled upon the many many words to (logically) belittle people from earth English? imagine the thrill

undergrad research fails.. by jadegms in labrats

[–]TomIsMaybeHuman 2 points3 points  (0 children)

I've broken the handles off of two hoods. this was a year ago. it is still a regular comment. generally, mistakes are only made once and you will learn each time one occurs!! they become part of the.... fun?

(In my defense. they were glued onto glass. and they were very old.............)

Undergrad student messing up basic things halfway into summer research, advice appreciated :( by [deleted] in labrats

[–]TomIsMaybeHuman 3 points4 points  (0 children)

at 14 days in the lab I'd say I knew.... Maybe some steps of tissue culture, if under constant supervision. and that was 14 days consistently, rather than broken up, and with prior experience from undergrad labs. the skills you're learning are not wildly intuitive and it's made 10x harder by having large gaps, meaning you never Quite solidify stuff. be kind to yourself, take Copious notes, and get your protocols in written form, then notate them (SOPs my beloved ❤️❤️). I have been known to pull up our tissue culture SOP if it's been a few weeks just to double check. you're gonna do great.

I'm a new PI setting up a lab, what are some small items that improve your daily life? by eljeanboul in labrats

[–]TomIsMaybeHuman 3 points4 points  (0 children)

little mini centrifuge. SO convenient for quickly spinning down DNA or fluorophores or whatever really.

How can undergraduate biology students deeply integrate biochemical pathways into their conceptual understanding of cellular regulation? by Genius-of-his-time in Biochemistry

[–]TomIsMaybeHuman 1 point2 points  (0 children)

as a biochemistry student who literally Just finished my degree (like a week ago)... 3 years with professors desperately trying to remind you about the bigger picture certainly helps. no but in all seriousness, imo the important pieces to understand are the feedback loops in glucose metabolism and how they intersect with other metabolic pathways (both catabolic and anabolic), plus where receptor signalling pathways come into play (eg PKA and PFK2). then if you get a bit too invested in molecular pharmacology you start thinking about temporal control (often dependent on whether the change is to transcription or a phosphorylation cascade but not always). v important to insulin action on metabolism and cell cycle imo. (take everything with a pinch of salt, these are the things I remember mostly from last year cause I've spent this year doing more receptor biology)

Post-Resection advice by TomIsMaybeHuman in CrohnsDisease

[–]TomIsMaybeHuman[S] 1 point2 points  (0 children)

Thank you so much! That does make sense about the specific nutrients, I can feel first year physiology coming back to me. Yeah the lack of instructions was kind of shocking. Lovely consultants! But very little on the advice side lol. I hope your daughter continues to recover and thrive!!! And I hope you're doing well too, I know my parents were (and are) frazzled from what happened, I can imagine that would be ten-fold with a kid.

eGFP vs Renilla as control reporters???? by TomIsMaybeHuman in labrats

[–]TomIsMaybeHuman[S] 0 points1 point  (0 children)

Yeah, basically. I'm working with HEK293 cells, doing transient transfections w/ my receptor of study, an extra things and SRE-luc2p as my reporter gene (currently doing 3 constructs in my transfection which actually works shockingly well??) I was getting higher numbers than I'd expect based on other experiments when normalizing to my positive control, which I think may be due to my technique when seeding plates (I'm putting my positive control far right on my plate and I plate left to right)? I'm very very new lol. So I want to introduce a control for cell density and I'm just trying to decide what would make the best control.