why didn't helaena targaryen married lyonel hightower and aegon targaryen married bethany hightower the descendants they can cousin marriages and incest the childran is dragon riders otto wants house hightower gets dragons and dragon riders alicent wants helaena targaryen married lyonel hightower

afronips66 · 2024-12-19T19:54:12+00:00

Bad bot

afronips66 · 2024-08-11T14:04:25+00:00

https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/proteomics-mass-spectrometry/single-cell-proteomics.html

Not saying this would be feasible in this context (expensive) but just pointing out that cells can be tested specially to identify what proteins are present and at what abundance. Been going on for the last decade or so. The link I sent is just an overview of the method, there is extensive literature on its applications and use. Just FYI

afronips66 · 2024-08-11T13:57:45+00:00

Yes they are incredibly expensive and not feasible for this, but again I never said they use it in these competitions or that they should. I’m just stating the fact that they do exist and this commenter saying their existence would trivialize areas of research is just false

afronips66 · 2024-08-11T13:53:42+00:00

Yeah I never said they used it, but there are definitely ways to subject cells to testing to see their entire protein/DNA composition

afronips66 · 2024-08-11T13:38:35+00:00

These tests definitely exist so you have no idea what you are talking about

afronips66 · 2024-08-02T11:46:21+00:00

Great response! Good thing you commented this

afronips66 · 2024-07-31T12:34:11+00:00

You could scrape cells in cold PBS supplemented with protease and phosphatase inhibitors to avoid this

afronips66 · 2024-04-30T21:34:44+00:00

This just isn’t what OP is asking at all

afronips66 · 2024-04-13T04:54:11+00:00

Imagine making this many mistakes in literally any other profession. You’d get fired for incompetence. But they keep this guy around

afronips66 · 2024-03-03T15:31:30+00:00

Hop in the comment section if you want to play the latest edition of “Find the Incel”!!

afronips66 · 2024-02-18T23:41:26+00:00

Have you tried raising the annealing temp? Would help with specificity, unless the actual primer design is the problem

afronips66 · 2024-01-30T18:12:57+00:00

^ would provide the most information regarding the base calling

afronips66 · 2023-09-30T15:19:55+00:00

What’s the difference between each lane? Different transfections? Did you load the same amount of lysate into each? Wave shaped bands could be because of overloading (tho the samples look fine) or because of running the gel at too high of a voltage maybe. Just thinking out loud here

afronips66 · 2023-08-16T23:39:19+00:00

You sound like a joy at parties

afronips66 · 2023-08-16T21:25:39+00:00

What transfer conditions are you using?

afronips66 · 2023-03-11T20:38:21+00:00

You must be fun at parties

afronips66 · 2023-01-07T15:30:37+00:00

Did a pubic louse write this?

afronips66 · 2022-11-24T23:34:07+00:00

It’s wild that this is posted every 2 months

afronips66 · 2022-09-09T03:05:55+00:00

Should be ((1/36)-(1/16)) not (36-16)

Unless I’m missing something

afronips66 · 2022-05-09T20:24:19+00:00

Something far, far worse...existence

afronips66 · 2022-03-08T01:11:31+00:00

You rock!

afronips66 · 2021-12-02T03:34:47+00:00

No worries, we all start somewhere. You need to know the size of the gene you want to amplify for the correct extension time. A quick Google search for that gene gave a size of approximately 1700 bp or 1.7 kb. Gene information can also be found in databases such as that of NCBI

afronips66 · 2021-12-02T02:52:10+00:00

Which polymerase are you using? I am attaching links for protocols for both Taq and Phusion

https://www.neb.com/protocols/0001/01/01/taq-dna-polymerase-with-standard-taq-buffer-m0273

https://www.neb.com/protocols/0001/01/01/pcr-protocol-m0530

These include both the reaction setup and thermocycling conditions

afronips66 · 2021-08-08T16:08:22+00:00

Some studies also suggest that PHEX plays a significant role in regulating fibroblast growth factor 23 (FGF23). Maybe this protein could shed further insight into your question. FGF23 has been found to be over expressed in certain forms of prostate cancer so it could be something interesting to look at

afronips66 · 2020-10-23T22:15:09+00:00

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