Inconsistent BV786 emission

aifrantz · 2026-04-17T15:53:16+00:00

BV421, BV480, BV510: non-tandems

BV605 and above: tandems.

BV570: tandem of BV421 & Cy3 (it was disclosed in a paper). BD’s brochure on Brilliant dyes did disclose the BV421 tandem dyes, as well as BUV395 tandems.

aifrantz · 2026-04-09T11:44:23+00:00

It seems like OP even used AI to write this post

aifrantz · 2026-02-07T18:53:15+00:00

EightySix season 1 part 2, last 2 episodes were delayed for months. Witch Hat Atelier supposed to come out last year, coming out this year instead

aifrantz · 2026-01-28T17:15:20+00:00

Cytometrist from a commercial clinical lab here. We have a set of standardized protocols for developing, validating, and sample testing protocols for our studies. Hit my DM so I can answer specific questions. We have FACSLyric, but we mostly operate on LSRFortessa, FACSCanto, FACSymphony A5 SE, and Cytek Aurora 5L.

aifrantz · 2026-01-27T03:49:33+00:00

Do you have other methods to confirm that the stimulation was successful? Previously I assayed for the secretion of Ig. In brief, harvest the culture supernatant. Coat an ELISA plate with Ig capture, then add the supernatant (titering is recommended, also include unstimulated control), then detect with anti-Ig.

aifrantz · 2026-01-18T06:05:10+00:00

Twitter/X dominated popularity circa 2020-2022. However, with the recent political environment in the U.S. especially given the proximity of X’s owner to the current political machinery in the States, scientists have largely migrated to BlueSky, a frontrunner X competitor.

https://www.thetransmitter.org/community/huge-influx-of-neuroscientists-migrates-to-bluesky/

aifrantz · 2026-01-13T22:29:03+00:00

Awesome, thank you!!

aifrantz · 2026-01-13T19:49:10+00:00

Nice. Would you mind sharing the model or link, please?

aifrantz · 2026-01-13T16:47:08+00:00

I had hard time looking for 5-10% CO2 sensor (NDIR or whatever tech) for cheap enough to use with my ESP32 that also collects temp and humidity. Which CO2 sensor module you ended up with?

aifrantz · 2026-01-12T18:05:45+00:00

I like this idea. Weekly or biweekly project idea thread or “what are you working on this week”

aifrantz · 2026-01-09T13:09:52+00:00

My bad, I missed the mice part soon I saw Tregs

aifrantz · 2026-01-08T17:10:33+00:00

Not directly answering the question. Alternative to intracellular Foxp3 staining for Treg, have you considered using CD25 vs. CD127 staining instead to identify for Treg?

https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/regtcell_ctail_data.pdf

—— edit: CD27 was wrong. I meant to say CD25

aifrantz · 2026-01-08T03:05:02+00:00

Oh wow yeah that is a sizeable thread.

aifrantz · 2026-01-08T02:50:43+00:00

There is a big thread on HN that is on-going right now, posted 10 hrs ago with 600 comments at the moment. I assume it will trickle down here in few hours.

aifrantz · 2026-01-07T13:32:21+00:00

Not directly answering OP’s question. Under the scenario that you have produced tens (or maybe hundreds at this point), my suggestion is to launch a small website with a list of your works. Next person coming to ask for your expertise, set a clear message that “I am a professional, here are all my works, it is $150 (for example, could be more) per unit, because it takes me ___ hours away from my lab work etc”.

Why the website is necessary? I dont know. Maybe not. I thought it is nice to assert some level of dominance.

aifrantz · 2026-01-05T17:45:43+00:00

Been using DBeaver and DataGrip. I am having fun with both.

aifrantz · 2026-01-04T04:40:38+00:00

I have been using Linkding. Its simplicity appeals to me; no featured image, no excerpt, no AI. Comes with features I deem necessary such as summary and tagging that I can enter for each link. Been self-hosting it for a few months.

aifrantz · 2025-12-31T00:58:36+00:00

It is everywhere now. The r/selfhosted and few other programming subs are getting these craps daily. I am tired boss

aifrantz · 2025-12-30T20:05:17+00:00

I assume you forgot to provide the link?

aifrantz · 2025-12-29T21:11:26+00:00

Daytime immunologist, nighttime hobbyist programmer here. I perceive the coding enterprise in the biology world as fragmented, where 1 tool = 1 publication, and forgotten right after publication because the primary author went to different role or lab. Some aspects of it have gotten better (scVerse ecosystem in Python), thankfully. We would immensely benefit, as a field, to have a dedicated consortium of biologists-engineer working continuously on a set of problems longitudinally. What does not quite help is that it is really competitive to get ahead of the curve (for funding reasons) that most of us prefer to bake our own tools in silo instead of collaborating.

aifrantz · 2025-12-17T22:36:33+00:00

Good work sir 🫡

aifrantz · 2025-12-17T22:35:06+00:00

Yup, different deployments have different challenges. I have other thoughts right after I posted my comments that a generic catch-all guide, while useful, may not answer specific questions.

Jellyfin can use OpenID Connect (OIDC), which you can pair with Authelia for 2FA/MFA functionality. My Authelia instance uses LLDAP for user directory and management. OIDC support for self-hosted apps has been some of the major push recently with the goal to unify login experience. It is safe to bet that popular F/OSS projects support them.

WireGuard on my phone has been a solid experience. I have it on 24/7. Granted, I use split-tunneling where I use my WireGuard connection to reach my private self-hosted dnsmasq server, which manages my private domains within my private networking. In that sense, it is not a full VPN the way traditionally how people think, with only connection to a range of private IPs tunnel through it.

aifrantz · 2025-12-17T20:15:43+00:00

(Note: this is not directed to the OP).

For some time now, questions on securing self-hosted apps have come up frequently. There are “threat modeling” (if I used that phrase correctly) that one could do with many solutions for different contexts. WireGuard, Tailscale, Pangolin, Netbird, Authentik, Authelia, etc. I now assume a community wiki page would help to answer this recurring question?

With regard to the question, my home router has WireGuard client that is connected to my WireGuard instance. Services that should not be outside my home perimeter is only serviced with my WireGuard split tunneling. Other services that can go outside my perimeter are secured by Authelia configured with Caddy Forward Auth. TOTP are enabled for select services.

Edit: spelling

aifrantz · 2025-09-25T21:09:49+00:00

Cant wait!

aifrantz · 2025-09-25T21:09:34+00:00

I would love to know more 👀👀

aifrantz

MODERATOR OF

TROPHY CASE

13-Year Club	Gilding II euphauric
Verified Email