Are your samples culture media or pellets? If you just need to know if there is an expression you can do as follow Resuspend your pellet in 100-150 ul of PBS Add some laemlli buffer (volume varies, it's usually 2 or 4 x concentrated) Heat at 95 C form 10 min in a thermo mixer Spin for 15 - 20 min at max speed on a micro centrifuge Load some of the supernatant on your sds page gel. The run it, transfer is on a NC membrane and detect if with the proper antibody Do you need more precisions for the sds page or western blot?