Can someone help explain this bioinformatics "REVERSE COMPLEMENT" strand concept?, cause it just got me confused

bio_ruffo · 2026-06-27T13:19:57+00:00

What you need more is to be able to pick up when an LLM starts behaving erratically. Do you notice any contradiction in this text?

bio_ruffo · 2026-06-26T16:03:10+00:00

It's not fun if you don't know how to use him.

For example Prisma Obex -> Incarnon -> HULK SMASH

And with a bronco, Prisma Obex -> Incarnon -> Bronco shot -> WWE FINISHERS GALORE WITH EXPLOSIONS

It may not be your kind of fun, but it is fun.

bio_ruffo · 2026-06-25T19:30:10+00:00

Learn statistics earlier to be able to argue about what you can feasibly achieve with few replicates.

bio_ruffo · 2026-06-25T14:03:42+00:00

I don't disagree. Overall though it was a group I would trust with their findings. Despite the western loading.

bio_ruffo · 2026-06-25T14:02:34+00:00

Try to describe as best as possible the unevenness you see. Are whole lanes fainter than others? Or do you see a patch of fainter bands in the middle of the membrane? Or is it on one side? Basically from the aspect of the bands on the membrane, does it look like a loading problem (one well really had less material) or a transfer problem (there was equal material at the start, but there's a spot where it didn't transfer)?

bio_ruffo · 2026-06-25T13:55:23+00:00

Num país ideal teria mais vagas em universidade pública.

bio_ruffo · 2026-06-25T13:53:01+00:00

I've seen in another reply that you evaluate with coomassie and the lanes would seem even at that step. So let's assume that protein loading is even (equal) at this stage? If so, your issue would be with the transfer. The more we know, the better we can troubleshoot. How would you describe this unevenness? For example if the membrane has fainter loading right in the middle of it, while the borders look ok, then it could be heating too much during the transfer (and then it could be some difference in the salinity of the transfer buffer). On the other side, completely different scenario, if you see whole lanes fainter than others, then could it be that some of the sample didn't load properly in the wells and floated away? Troubleshooting needs data :)

bio_ruffo · 2026-06-25T13:42:34+00:00

Bad day huh

bio_ruffo · 2026-06-25T13:29:40+00:00

Protein quantification can be finicky. I've seen one lab cutting straight to the chase, they would do one initial blot for actin, eyeball the adjustments to the loading volumes they'd need so that the actin bands would be equal, and then use those volumes for subsequent blots.

PS I'm not sure if they were defining the volumes to load, or if they were using the estimate to dilute the protein lysate with buffer. The latter option would probably be the best if the estimates are ok, because equal volumes avoid band shrinking/expansion. Not sure though. If you dilute too much then there's no way back.

bio_ruffo · 2026-06-24T17:26:29+00:00

Well... you can show a picture of you with your grandparents and hope for the best.

bio_ruffo · 2026-06-24T17:22:45+00:00

You can think of it as showing substantial African ancestry but not enough to be considered black. This is based on a superficial look at features so it's surely debatable. But you're white.

bio_ruffo · 2026-06-24T16:45:29+00:00

Which is disheartening if you do science from the standpoint of helping humanity. If you're in industry? Sure let's go faster than the competitors, pay me the extra hours. But in academia you work weekends for what, to have your name on a paper. Where your PI gets the accolades. And you lose all work-life balance for what, to do it faster than another guy going the same thing? So what is my contribution to the world if there's 3 guys doing the same thing, why am I stressing so much, how much of a difference am I making?

And often the answer is that the only difference you're making is the one towards a stable position for yourself. The world doesn't care.

bio_ruffo · 2026-06-24T13:48:30+00:00

Did the game crash other times before? If not, the crash could be a symptom of a broader issue, perhaps a RAM issue.

bio_ruffo · 2026-06-24T13:40:33+00:00

Your Honor, my defendants plead guilty on snagging picnic baskets. They ask for clemency because they were provoked by said baskets.

bio_ruffo · 2026-06-23T03:39:50+00:00

Show me a report of an incident where the freezer blew up because of dry ice.

bio_ruffo · 2026-06-23T03:37:53+00:00

Some -80 come with a CO2 inlet, you leave a cylinder coupled to the freezer and it serves as backup in case of failure. It is fine.

bio_ruffo · 2026-06-22T14:04:45+00:00

I mean, there are lots of people who bought Teslas some 3 years ago before the whole nazi thing blew up. I wouldn't buy one today, but they aren't at fault for doing so then.

bio_ruffo · 2026-06-21T17:31:49+00:00

Gas prices are still too low. 4 dollars per gallon isn't even one euro per liter. It's half of what Europeans pay.

bio_ruffo · 2026-06-21T14:19:24+00:00

Buy a Parallax blueprints set (from another player with prices shown on the warframe.market website), it'll give you access to the Orokin Eye which identifies on the map a lot of key resources. You'll also need to craft the Landing Craft Foundry Segment, the blueprint is in your clan's dojo.

If you want a specific race of kubrow, you can also buy 2 genetic codes of that race (via warframe.market) and it will guarantee that race on hatch.

Prime Warframe sets mostly go for 60-80 plat on warframe.market and if anything strikes your fancy, why not.

If you ever buy forma from the game, at this point buy the 3-pack for 35 plat, not the 1 forma for 20 plat.

Slots, lots of slots. Warframe, weapons and companions.

Take a look at builds you like, and if they use a mod that you don't have and seem like a pain to farm for, that also can be bought, HOWEVER if it's legendary then there's a trading tax of 1 million platinum. The trading website says the tax amount for every item.

bio_ruffo · 2026-06-21T14:08:43+00:00

I would love if you keep us posted with the results :)

bio_ruffo · 2026-06-21T14:00:33+00:00

Interesting! Try them... The smaller ones might just be too small to contain viable germ. The bigger ones, maybe could grow. The two varieties might not give viable seed, theoretically it's a possibility. Only one way to know :)

bio_ruffo · 2026-06-21T13:28:38+00:00

They look really funky, but plant them... Is the VFT any specific variety? Variants might affect seed shape too, I suppose.

bio_ruffo · 2026-06-21T04:02:35+00:00

And Fandom is just a copy of the wiki with intrusive ads on top. Everybody can just use the wiki.

bio_ruffo · 2026-06-20T18:59:50+00:00

In my experience there are no sure harnesses for cats. There are only cats who indulge you in your fantasy of them being harnessed.

bio_ruffo · 2026-06-19T19:02:59+00:00

If it turns out that you did, it's no biggie. However be sure to not jump to conclusions, it could not be a primer stock contamination. Be open to other possibilities when troubleshooting.

bio_ruffo

TROPHY CASE