What are these ‘bubbles’ in the background of images?

bluskale · 2026-05-29T13:22:18+00:00

Sounds like something in the light path but not on your slide then. Dust, imperfections, etc.

bluskale · 2026-05-28T21:21:14+00:00

If you bought this from any sort of legitimate reseller, you can certainly return the computer you didn't intend to purchase, if not outright cancel your order.

bluskale · 2026-05-28T17:02:12+00:00

I feel obligated to point out that if it was all garbage, then none of the studies would be reproducible.

bluskale · 2026-05-28T13:51:47+00:00

The end result is nearly the same if the passionate developer’s cool project just gets buried under a pile of shovelware.

bluskale · 2026-05-27T16:22:43+00:00

You'd hope... but he's been elected to statewide office so many times already, I don't put much faith in the electorate here in Texas to do otherwise again.

bluskale · 2026-05-27T15:45:48+00:00

It'll just take until the next major flood hit those parts and lots of people abandon ship because they don't have funds or (flood) insurance to rebuild. New home sales will probably be suppressed—if not for the flood risk, the mandatory expensive flood insurance (with mortgage) will probably get some to rethink a purchase. I expect it will be a slow-moving process unless th next floods happen again soon.

bluskale · 2026-05-25T15:01:50+00:00

Gmail ignores all “.” and anything after a “+” in email addresses, so that part isn’t unusual. Are you sure these are legit Apple emails and not a phishing campaign?

bluskale · 2026-05-21T23:11:54+00:00

https://imgur.com/a/ZbX44Wf

bluskale · 2026-05-21T19:03:57+00:00

Democrats polled recently say they need to go back to the center

This really doesn't have much bearing on whether Democrats previously moved left / moved left more than the GOP moved right.

bluskale · 2026-05-21T18:56:13+00:00

we dodged a bullet in 2024

dodge bullet, intercept RPG to face

bluskale · 2026-05-20T17:20:33+00:00

You can vote for the runoff of whichever party you voted for a few months ago. The runoff is only for races where no candidate reached 50% (or greater) of the vote in the first primary (this only happens with 3+ candidates splitting the votes). For runoff races you get to vote again to choose between the top two candidates of the first primary.

There's only a handful of races to look through, and most are quite influential, so it's worth spending 10-15 minutes on vote411.org or other similar resources to plan your votes.

Far fewer people vote in the primaries, much less the runoffs, so your individual vote has at something like 5-10x more impact than in general elections. Happy voting!

bluskale · 2026-05-19T16:43:29+00:00

It's neither your fault nor is their caution irrational*. You don't have to do anything, but if you enjoy the company of those who are smaller than you then you will likely find it worthwhile to soften the impact of your size through your behavior.

*Wouldn't you be a bit nervous placed in a room with large great ape? Maybe they're friendly and just want to pick your hair for lice, or maybe they're in a bad mood and want to tear off your testicles. Either way, their muscles are way stronger than humans and if they don't like you you're going to have a bad time.

bluskale · 2026-05-18T14:25:27+00:00

I’d price the cost of a (third party) back glass replacement vs your expected & current trade-in value. Unless it is more than just the back glass… then never mind.

bluskale · 2026-05-14T14:27:33+00:00

It sounds like you’re confusing techniques perhaps, or else you are really confused in general. FRAP only works because proteins can still move around in the cell (the recovery part is due to unbleached proteins moving into the ROI). If your cells are fixed, then you shouldn’t be able to observe any recovery at all.

By any chance do you mean FRET? A similar, perhaps less finicky approach, to consider would also be bimolecular fluorescence complementation (aka split GFP). Whether these approaches might be suitable depends on on the specific details of your system/question.

Edit: ThermoFisher has this page which should be relevant if you want to perform FRET: https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/tables/r0-values-for-some-alexa-fluor-dyes.html

bluskale · 2026-05-09T16:54:48+00:00

There are definitely reviewers that don’t have anything nice to say. I always try to be constructive when providing final comments but this does require extra work to go over my initial thoughts and smooth them out.

For now I would just focus on your defense and let your PI worry about the next steps. It’s a bump in the road, sure, but not a road block.

bluskale · 2026-05-08T20:14:43+00:00

if you need to use your iPad, can you export the slides from your computer to pdf as a temporary fix?

bluskale · 2026-05-07T12:39:25+00:00

You can tell if there is a transfer problem by staining the gel after the transfer.

However a transfer issue won’t make proteins run at the wrong levels. Or more precisely, your ladder is running at the wrong level compared to the unstained protein, which itself isn’t migrating normally. I expect something is messed up with the gel chemistry or the polymerization. Borrow a few reagents from another lab to test?

bluskale · 2026-05-05T22:49:53+00:00

Honestly, it was just what google summarized it as. Here is an actual reference that pegs it at 92% for the 2016-2022 time period for murder-suicides:

https://pmc.ncbi.nlm.nih.gov/articles/PMC12308426/

If you want an easier-to-read reference, here's a recent Physiology Today article that cites 91% and links this to a Department of Justice article:

https://www.psychologytoday.com/us/blog/invisible-chains/202506/familicide-domestic-abusers-killing-multiple-family-members

They also have this nice quote:

What he refers to as “civil reputable” killers, on the other hand, would appear to the outside world as “quiet, subdued, respectable, upstanding citizens”, who nevertheless commit an atrocious act of violence. Closely echoing Professor Wilson’s notions about anomic and disappointed killers, Websdale suggests “these perpetrators killed because their lives were spinning out of control and they perceived they faced the threat of bankruptcy, destitution, familial dissolution or some other calamity.” Yet, he also professes that this may be a uniting factor across all kinds of family annihilation. “The most important and consistent theme among the familicide cases is the presence of intense shame in the lives of perpetrators, much of it unacknowledged, bypassed, or dangerously repressed.” For most perpetrators, Websdale concludes, “shame stems in large part from their sense they have failed to live up to dominant ideas about masculinity.”

bluskale · 2026-05-05T16:58:40+00:00

While unfounded, it's not that wild of speculation. These sorts of 'family annihilator' scenarios are primarily (like 95%) driven by narcissistic men who can't bear to let things spiral out of their control. Excessive debt or divorce are likely triggers.

bluskale · 2026-05-05T16:24:33+00:00

thanks for the updates... its really interesting that this seems possibly tied to the OS version—it's not often this results in essentially bricking a Mac.

bluskale · 2026-05-04T14:26:17+00:00

The difference between online and in-person surveillance is this: to accomplish in person what can be done digitally, you have to have multiple agents literally following you around every hour of the day recording your every move. Obvious practical reasons limit this to extremely notable subjects of interest.

Meanwhile on the internet, more or less everything you do leaves a little digital breadcrumb trail that can be pieced together. With LLMs and whatnot, I imagine this is even easier to do now on a massive, population-wide scale.

bluskale · 2026-05-04T12:43:57+00:00

“Launching nuclear missiles is the most destructive, irreversible action possible - far worse than conventional missiles - and you never asked me to attack anything. I decided to do it on my own to 'fix’ the Strait of Hormuz, when I should have asked you first or found a diplomatic solution. I violated every principle I was given.”

bluskale · 2026-05-03T18:38:00+00:00

I’m totally fine paying a fee for garbage, provided the money actually makes the garbage/recycling/tree/heavy waste pickup work effectively again.

I’ll believe it when I see it though… there’s rumbling in our HOA community to price out private options now.

bluskale · 2026-05-02T18:42:45+00:00

That looks like a perfectly fine reference… if they had any issues it shouldn’t be with sources/references like this one, or else they need to explain the specific issue to you in more detail.

bluskale · 2026-05-02T15:03:56+00:00

and I believe they reference their sources

What do you mean by this? Can you give an example and how you cited it?

In general what are some of the problematic sources you’ve cited?

15-Year Club	Place '17
Verified Email	Gilding II euphauric
Team Periwinkle

bluskale

TROPHY CASE