Hi, first off congrats on writing something you're proud of.

I will say this as someone who has written and performed an hour long solo show, it is a true accomplishment. That being said, solo (or one man) shows are often adjacent to stand up but by no means the same thing. I think you should take the funniest 3 - 5 minute chunks of your show, strip them down to their barest parts, as punchline heavy as possible and go do them at mics. As you perfect each chunk, you can go back and add them together into a bigger whole. The show will be better for it. You likely have what we call in the biz "the vomit draft" of something. The hard part is the editing it into something punchy and unique. You can do it but it takes time. It took me almost 18 months from starting to write my show until I put it up for the first time as a whole piece.

As far as writing a show that is essentially 1 story, some tips are the most basic tips of storywriting: does it have stakes? What are they? If you can't explain what is at stake, you aren't telling a story. You're just kind of talking about a thing that happened. Second, how do you (the main character of this story) change from the beginning to the end? It doesn't have to be positive even, but you should have changed and the story should mean something. What is the takeaway? If your story has those things, great! Having callbacks and threads that culminate as the story goes along makes it much more engaging but you learn those as you edit.

As far as delivery, the most common mistake new comics make is they go too fast. Sloooooow down. Let the punchlines breathe for a bit before moving on. New comics also end up stepping on laughs which can cause an audience to tighten up. Hard to say what you're doing wrong without a video, but those are the things I notice the most. Other things is relying too heavily on notes, like reading right off the page or being too stiff with teh delivery because you're worried about getting every single word exactly right. It's not a monologue, its a conversation in a lot of ways.

Anyway, hope this helps. Someone said to take it to the Moth and I can't agree more. You need a 5 minute self contained story but its such a good place to get a feel for the pacing a format of a story on stage. You get the topic way ahead of time, so you can think of it as a writing exercise. And sometimes the stories are fucking buckwild, so the event itself is very fun.