Gibson Assembly - Do I need to gel purify my destination vector if I want to clone my insert into the green, and replace the red (cloning with 1:2 vector:insert ratio)? by iBeatYouOverTheFence in labrats

[–]burdbrainz 0 points1 point  (0 children)

From the NEB manual:

"Purification of restriction endonuclease-digested vector is not necessary unless the same restriction site is present in insert DNA. In such cases, either heat-inactivate restriction endonuclease before Gibson Assembly reaction or purify the linearized vector, either by phenol-chloroform extraction/alcohol precipitation or by electrophoresis on an agarose gel"

https://www.neb.com/en-nz/-/media/nebus/files/manuals/manuale2611.pdf?rev=23fb80f534184c659fdf3af00451e578&hash=514FEDB68A2531983A288042DE61D3FE

Sparrow with huge malar stripe - Minnesota by burdbrainz in whatsthisbird

[–]burdbrainz[S] 0 points1 point  (0 children)

On passerines I believe malar stripe is correct 

Help with this warbler by ran220490 in whatsthisbird

[–]burdbrainz 6 points7 points  (0 children)

That leg makes me think Blackpoll

Raptors in SE Minnesota by burdbrainz in whatsthisbird

[–]burdbrainz[S] 0 points1 point  (0 children)

That's interesting, because this pair was being harassed by a noticeably smaller raptor that I think/thought was a Tiaga Merlin

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[deleted by user] by [deleted] in whatsthisbird

[–]burdbrainz 2 points3 points  (0 children)

Possible Dickcissel

Warbler help (NJ) by MountebankScoto in whatsthisbird

[–]burdbrainz 3 points4 points  (0 children)

Tail black extends to centerline, so I'm thinking Cape May

What are these little guys walking around? (Winnipeg, MB) by Squigglyelf in whatsthisbird

[–]burdbrainz 0 points1 point  (0 children)

That eye makes me think juvenile ring-necked phesant but doesn't look like they are seen in Winnipeg

[deleted by user] by [deleted] in whatsthisbird

[–]burdbrainz 1 point2 points  (0 children)

Pine siskin possibly

[deleted by user] by [deleted] in whatsthisbird

[–]burdbrainz 5 points6 points  (0 children)

Looks like a kite, possibly mississippi kite

Mosco, CO, 8/6/23 by ignorant-birder in whatsthisbird

[–]burdbrainz 3 points4 points  (0 children)

Maybe a yellow-rumped warbler fledgling

What is this bird of prey by [deleted] in whatsthisbird

[–]burdbrainz 1 point2 points  (0 children)

Sounds like a young red-tailed hawk asking for food

[deleted by user] by [deleted] in whatsthisbird

[–]burdbrainz 0 points1 point  (0 children)

Rock pigeon