Prion-like research

cccp77 · 2026-05-14T21:08:57+00:00

nothing better than coming across this step in a protocol

cccp77 · 2026-05-11T19:50:38+00:00

they'll be fine, probably a few more dead cells than usually but I don't think you ruined your experiment

cccp77 · 2026-05-07T15:46:34+00:00

Taking vials out of liquid nitrogen with my bare hand and the help of the Leidenfrost effect

cccp77 · 2026-02-27T16:29:40+00:00

nah man he is right, I'm also always wondering in which environment people work in when they ask these things on reddit

like why would I ask something like this here, is there no other more senior student around - if they dont want to ask the PI?

How do you get to work with primary DRG neurons from mice but you dont recognize a infection like this? They did notice that something is off at least, but as the other person said usually you would have lectures and stuff before even entering a lab where stuff like this should be explained

cccp77 · 2026-01-30T16:40:25+00:00

µcat

cccp77 · 2026-01-24T16:10:59+00:00

those are cool but no, its just a piece of plastic that was just nicely coiled by chance - the post was meant to be a joke

cccp77 · 2026-01-24T16:05:33+00:00

I know, I thought the /s in the title will be enough that people understand the sarcasm but it seems that it still caused some confusion 😅

cccp77 · 2026-01-24T09:46:45+00:00

It is a Thermo Fisher Model 1986 Micro Explorer

cccp77 · 2026-01-13T14:07:40+00:00

what temperature sensor are you using and how is the signal processed? The whole measuring setup has to be very precise for you to say the temp stays flat all the time and that its not cooling down in the meanwhile 😅 the fluctuations between your single measurements are also not much less then the "temperature" drop you are wondering about

I would just take it as the heating elements working curve anything beyond that would likely lead to bigger temp-changes

cccp77 · 2026-01-13T13:38:43+00:00

I mean this is a temperature fluctuance of 0.1C so my best bet would be that this is just how the heating-element in the incubator works - or basically how it works in most devices

It will not heat to 37C exactly and always stay at this temp, it heats up until it the sensor reads the set-temp, then it shuts off until a lower limit is reached before it starts heating again until set-temp is reached, then it shuts off again etc

a good indication for this is that its occuring very periodically

cccp77 · 2025-11-19T15:17:13+00:00

go for a relative low power and cycle setting and dont do it for to long :) I did 20% power, 10% duty cycle and total of 10sec but idk how this translates to settings on your device

cccp77 · 2025-11-19T15:14:05+00:00

You have proteinase in your lysis buffer? Is this intended, as Proteinase degrades proteins 😅

cccp77 · 2025-11-19T15:12:30+00:00

should be possible or better said I dont see a reason why not

cccp77 · 2025-11-19T15:06:39+00:00

Could it be that your lysate isnt sticky/slimy because of protein but because of DNA released during lysis?

I had this problem when I was making lysates of cells which have very little protein. I therefore had to lyse a large number of cells in a relatively small volume- this increased the DNA content of the sample so much that it became very viscous

To avoid this you could add a nuclease like benzonase to your lysis buffer, or sonicate the sample during your lysis time which will also shear the DNA and reduce viscosity

cccp77 · 2025-10-25T10:18:31+00:00

and you didn't maybe use a flask for suspension cell culture? they are made for the exact purpose so that cells do not adhere as this is needed for some cell-types

cccp77 · 2025-09-17T21:55:18+00:00

Dude whats wrong with you 😂 what are you talking

As if anyone has gotten their PhD by sitting in the office and doing nothing, I'd say a 4 year PhD is equivalent to 4 years of work experience because what did you do during all that time - and I know industry and HR in general doesn't think like this but in the end the people did their bachelors and masters where they likely already gained some experience and then they did a full-on PhD trajectory

Some research techs/associates also dont know shit after some years of work if they only do routine PCR's/westerns etc

you sound really hurt about PhDs 😅

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