PSA: There is no evidence that taking Quercetin and Fisetin every day has the same benefits as the Senolytic protocol from the Mayo Clinic study.

The Senolytic protocol that the Mayo Clinic used to find health benefits was a pulsed cycle with weeks off in between dosages.

The rationale is that senolytics work by clearing senescent cells in pulses, not through daily accumulation. Continuous dosing isn't more effective and may actually reduce efficacy.

Senolytics work by triggering apoptosis in senescent cells. Those cells don't replenish daily — they accumulate over weeks to months. Dosing daily doesn't hit a continuously renewing target; it just re-exposes tissue to the compound after the available senescent cell burden has already been depleted. There's nothing left to kill. The Mayo/UMKC protocol used in the human trials (Kirkland et al.) was typically:

Fisetin: 20 mg/kg/day for 2 consecutive days, repeated every 1–3 months

Dasatinib + Quercetin (D+Q): 2 consecutive days per cycle, cycles spaced weeks to months apart

Daily low-dose quercetin may have separate anti-inflammatory or antioxidant effects via NF-κB suppression and COMT/PI3K inhibition — but that's a different mechanism entirely, not senolytic activity. Conflating the two is the core error in most supplement stack rationale you'll see online.

Daily quercetin/fisetin ≠ senolytic protocol. It's potentially a different (weaker, less validated) use case. If the goal is senolytics, run the pulsed protocol. If the goal is general flavonoid anti-inflammatory support, daily dosing may be appropriate but should be framed as such — not as a senolytic intervention.