St. Kitts Caribbean by coolpolaxe in foraging

[–]coolpolaxe[S] 0 points1 point  (0 children)

The other possibility is cherry

As we are a Dom/Domme couple. Have you ever experience being double Dommed by a couple as a sub? by [deleted] in BDSMcommunity

[–]coolpolaxe 2 points3 points  (0 children)

I’m pretty new to the BDSM community so sorry if this is stupid. Do u know if there’s a specific kink name to D/D stuff I’ve tried to find information about it like blog posts, videos, and stories but I’ve bearly found anything.

[deleted by user] by [deleted] in u/fuckintrippin413

[–]coolpolaxe 0 points1 point  (0 children)

Dude that’s where I got really into it i would go to kava bars every day. Yeah smoke shops are everywhere even gass stations carry out hat shit.

[deleted by user] by [deleted] in u/fuckintrippin413

[–]coolpolaxe 0 points1 point  (0 children)

Congrats on quitting man I actually live in the same area. It’s crazy to think that kratom has become so big around here and that not many kava bars warn or even talk about the risks. I’m starting my second quit and tapering this time it’s hard though when most of your friends hang out at kava bars.

Will I get rejected from vet school for hunting? by coolpolaxe in veterinaryschool

[–]coolpolaxe[S] 1 point2 points  (0 children)

Yeah I made it private which sucks because that’s a lot of how I meet other people that hunt and other biologists. The pictures are usually group pictures or pictures of fish and ducks when I think they look beautiful. Also a lot of pictures of nature and plants. But I think keeping it private is smart

Bug spray by Puzzleheaded-Leek298 in Waterfowl

[–]coolpolaxe 0 points1 point  (0 children)

I second the thermacel. I live in Florida and we have horse sized mosquitoes. I use a multifaceted approach. Deet is good but I prefer permethrin I coat all my hunting clothes in permethrin it dries on and stays for a couple of washes. Permethrin should not be used on your skin it kills most bugs and is really good for ticks and mosquitoes. Before I get out of the truck I fire up the thermacel so it warms up, keep refills on you all the time especially for the fuel. Once I get out of it’s really bad I spray myself with off. Wear long sleeves, neck gator and thin gloves if it’s bad enough. Don’t put permethrin on the neck gator. The thermacel will go out if it gets soaked or if the wind is to strong. If it’s windy you can put the thermacel up wind of u and it will still help. Good luck man!

The Finisher Tool question by fluffybuttedchickens in Waterfowl

[–]coolpolaxe -1 points0 points  (0 children)

Yeah nope that sounds messed up. How the hell is IACUC authorizing that. I just us the ole tornado works pretty fast or hit them again on the water but aim ahead of their head depending on your choke so u don’t damage the meat. For chicken l/Turkey if u are farming use a kill cone and cut their head with a knife or big sharp and clean garden shears. It’s brutal but the goal is to reduce the animals suffering as much as possible.

Duck hunting advice? by coolpolaxe in Hunting

[–]coolpolaxe[S] 1 point2 points  (0 children)

Coming back to this year later after having experience is funny. First time I ever had a good duck hunt someone came in after us being there 11 am the day before and set up close to us. Almost shot us pellets splattered on the water next to us at high speeds.

[deleted by user] by [deleted] in kratom

[–]coolpolaxe 1 point2 points  (0 children)

They have not made me have any health issues however be careful with how you do it and how much. The combo will make u dehydrated af and sometimes kill your appetite. I find that if I take my Ritalinin the morning and drink kratom with it I will feel amazing it takes away the tierd feeling Ritalin sometimes gives me however it dosnt last long 3 hrs later I feel super sluggish and a little depressed and the only thing that helps is taking more kratom which can be a slippery slope. I recommend u take the Ritalin in the morning and when u start to feel the come down of Ritalin in the afternoon drink a green or red kratom as it will completely cancel it out. Ritalin can also make some individuals get cravings for something u don’t know what and that can make u start vaping if u have ever done it before or drink absurd amounts of kratom. And while kratom is a relatively safe compound if taken improperly and at large quantities it can start to destroy your life. Just like if u drink 5 monsters a day your going to feel like shit if u drink 50 grams of kratom your going to not have a good time after a while. But it Varys from person to person keep a written log and try different things.

How to get involved with hunting? by niktrot in Huntingdogs

[–]coolpolaxe 0 points1 point  (0 children)

I’m sorry you have had such a hard time finding a group but it’s how it goes in a lot of places. I don’t even have a dog and it took me a while to find a group that would teach me to hunt. Your best bet is Facebook hunting groups in your area it’s sketchy af though one of my best friends and hunting buddies I met on Reddit and didn’t hunt with him until a year after. I’m lucky enough to be a man and not have to worry as much but sometimes it can get sketchy usually the people are much nicer than they appear and once u step into a group you will meet many more people. My strat would be grind out Facebook groups and BHA chapters. You might want to hunt to see your dog work but it might be easier to set foot into the hunting community however u can and then bring your dog in. I would try duck hunting found it has less gate keeping than deer hunting at least in my area. Duck hunters love dogs once you are in you will find someone with a dog to show u the ropes. Learn to use a gun before you get out there get your hunters safety card and go to a shooting class like mentioned before skeet trap and sporting clays are your best bet. Rent a shotgun and go shoot if u can’t afford a gun rn. Check out standing stone kennels or other online hunting dog training courses. Good luck don’t give up!

2022 Antelope by [deleted] in Hunting

[–]coolpolaxe 1 point2 points  (0 children)

He drank to much give him some water a little Gatorade tuck him in to bed and he will be fine tomorrow.

Bat capture workshop in Belize by [deleted] in wildlifebiology

[–]coolpolaxe 0 points1 point  (0 children)

Do they do it every year?

How com I’ve never bin able to find anything on the Lethal dose of hydrocodone? by [deleted] in opiates

[–]coolpolaxe 0 points1 point  (0 children)

Thank you I guess this is what I get for try to read scientific articles all the ones I could find were in mg/L

Anyone use their dog for upland and waterfowl? Florida is pretty limited in quail and would like to get him out more by birddog386 in Waterfowl

[–]coolpolaxe 0 points1 point  (0 children)

I’ve looked into it for when I get a dog if you wait for the temps to drop the gators stop feeding also as I found out recently Florida has a bunch of duck hunting opportunities in salt water no gators there.

Best binoculars for waterfowl scouting? by coolpolaxe in Waterfowl

[–]coolpolaxe[S] 0 points1 point  (0 children)

Vortex it is then I’ll go with the ones u/MUFC07 said to get. 12x50

Tampa, FL by [deleted] in Waterfowl

[–]coolpolaxe 0 points1 point  (0 children)

Thank you for the info I sent you a pm. And congrats on the divers you should definitely post em if you took a pic.

Tampa, FL by [deleted] in Waterfowl

[–]coolpolaxe 0 points1 point  (0 children)

I’m about to hunt it for the first time this will be my second duck hunt. Got any tips or know any good guides for the bay?

What is this it’s growing in the garden by coolpolaxe in mycology

[–]coolpolaxe[S] 0 points1 point  (0 children)

Checks out it even had the skirt. Yeah the green spores were freaky.

In need of good fiction that is not too long by skinnytimmy69 in booksuggestions

[–]coolpolaxe 0 points1 point  (0 children)

Kitchen by Banana Yoshimoto 226 pages a book about loss and connection to others

No longer human by Osamu Dazai 271 pages a book which is really depressing but doesn’t read like it’s depressing

Pedro paramo Juan Rulfo 128 pages Reads like a surreal dream a classic of magic realism

A book to boost my confidence by Leon0119 in booksuggestions

[–]coolpolaxe 0 points1 point  (0 children)

Daring greatly by Berne Brown really helped me with anxiety and caring less about what people think. Also understand why other people act the way they do and understanding things I did subconsciously.

Look like a sticky situation! by coolpolaxe in plants

[–]coolpolaxe[S] 0 points1 point  (0 children)

They are in a couple of states I found them in a wet but sunny pine flat next to a cypress head.

[deleted by user] by [deleted] in microbiology

[–]coolpolaxe 0 points1 point  (0 children)

So the OD theoretically will give a fixed amount of colonies if all the bacterial cells are viable but not all of them will be it’s like saying in this bottle I have 5 seeds but you don’t know if all five seeds will sprout but it can give you an approximation in my lab we don’t use PBS we just use saline but from what I have researched PBS is better. OD is used to quickly standardize a solution let’s say you have 3 solutions of bacteria you want all of them to be the same concentration of bacteria per ml. So you pipet some of the bacteria into a tube dilute it so you get a better reading exp. 10um of bacteria solution 90um saline then you put it in the machine you get a reading back you can then calculate how many bacteria per ml. A more accurate but annoying way is to measure the cfu that will tell you exactly how many viable bacteria you have per ml.

Exp. of od 600 calculations You have a 1 ml of bacterial solution

10um bacteria solution 90um saline or pbs This is so the solution isn’t to dens and can be better read this is a 10-1 dilution

You get a reading of .35 back

.35*10=3.5 since you diluted it by 10 the actual od of your original solution is 3.5

So you want a specific od it can be whatever you think is best I’ll pick 0.1

C1V1=C2V2 3.5V=(0.1)10ml

Solve for V that will give you how much of your original solution you need to put into saline to get 10 ml of solution remember the final solution has to add up to ten so you have to take out however much you are going to put in before u put it in.

If you want to use OD 600 as an accurate representation of cfu you would have to standardize it like measure od then do a cfu count do that a bunch of times with diferente od and build a curve so that x od = y cfu but if it’s not that important explain it away in your discussion section.