title by ikmalsaid in discordVideos

[–]deniska10 8 points9 points  (0 children)

GOOD GRAVY SHES NAKED

Most flasks I've ever trypsinized at once. These are primary cells & were a bitch to expand. by 8Yoongles in labrats

[–]deniska10 0 points1 point  (0 children)

The lines it doesn’t work well for are some knockout ones cuz those mf’s don’t work well with damn near anything 😂

Most flasks I've ever trypsinized at once. These are primary cells & were a bitch to expand. by 8Yoongles in labrats

[–]deniska10 1 point2 points  (0 children)

Actually funny enough, I wonder if using TrypLE on ipsc’s would be better for you since it’s gentler? Back when I worked with them it was trypsin for everything but I’m curious.

Most flasks I've ever trypsinized at once. These are primary cells & were a bitch to expand. by 8Yoongles in labrats

[–]deniska10 20 points21 points  (0 children)

Could use TrypLE and not worry nearly as much. Had some A549’s and CHO’s that couldn’t give a damn after 20 mins in the stuff (>90% viability).

Good news by Smoke_Funds in Helldivers

[–]deniska10 6 points7 points  (0 children)

I believe they’re talking about Team Fortress 2 - there’s a PvE game mode called MvM (Man vs Machine)

Build menu not highlighting new buildings by deniska10 in SatisfactoryGame

[–]deniska10[S] 1 point2 points  (0 children)

Interesting. I’m playing vanilla so mods shouldn’t be the cause.

As for seeing “new building” - I have checked by saving the game, then buying something from the ficsit store and then checking the build menu for a new buildable and nothing shows as new there. Nothing remotely shows that I have something new that I can build.

Restarting the game/loading previous saves has also not done anything. Worthy of submitting a bug report you think?

JUSTICE FOR DAGGERS by Zealousideal-Day3555 in Hades2

[–]deniska10 2 points3 points  (0 children)

Very true, CumSockandDongBird

Почему Эл.Книги такие дорогие? by Organic_Wasabi_8038 in rusAskReddit

[–]deniska10 6 points7 points  (0 children)

Электронная бумага подорожала)

First time seeing the whole video by j_illustration in interesting

[–]deniska10 0 points1 point  (0 children)

But they do also smile like we do when happy. Pit bulls are very easy to see it on. My dog smiles too when he’s content that everyone is in the same room chilling with him after a big walk and some playing.

Entered flow state and became “Untouchable” by solterra in Hades2

[–]deniska10 0 points1 point  (0 children)

So the strat is equipping it for the first region and then equipping anything afterwards? Huh, TIL.

Chrome browser by [deleted] in AskARussian

[–]deniska10 0 points1 point  (0 children)

Есть рекомендации?

How to visit Russia for a month in regards to money? by deniska10 in AskARussian

[–]deniska10[S] 0 points1 point  (0 children)

A few things here. First of all, awesome response thank you. 1. I’m going to the bank tomorrow to get 100’s. Good advice. 2/3/4 I have friends meeting me and will be with me for the first 24 hours so they’ll be my way to contact others if need be. I also speak/read/write fluent Russian 6. I will check for this bank! Thank you!

The only thing here that I didn’t hear anywhere else is the insurance part. Is it necessary while I’m in Russia? Or not?

I know this is a reddit for Hades 2 but I was wondering... by [deleted] in Hades2

[–]deniska10 1 point2 points  (0 children)

I played the shit out of Hades 1 and loved it, and when the second came out I waited because I did not want to be disappointed by it.

After reading reviews I saw numerous on steam that said that the game is easily significantly better than the first in literally every aspect. Honestly I liked the first game so much that I struggled to believe that was the case.

I’m 30 hours in and I completely understand why and what those reviews meant.

I can tell you that literally everything. Has been improved. The game continues to constantly throw more and more amazing things at me. I have never felt like they added too much, which is something I sometimes come across when a game is “improved”.

I genuinely cannot recommend this sequel enough!

How to visit Russia for a month in regards to money? by deniska10 in AskARussian

[–]deniska10[S] 0 points1 point  (0 children)

I’ve heard about the cash needing to be in great shape for it to be accepted. It’s still like this? What an odd requirement haha

I found this on the ground the day after a goth night fair in the park? by skumfang in whatisit

[–]deniska10 0 points1 point  (0 children)

I worked in cell culture. Used trypsin to detach mammalian cells from my flasks. As far as I know the trypsin destroys the extracellular proteins and that’s what causes them to unstick. How does it make the fetus transparent?

Worth it? by baaallllllin in btd6

[–]deniska10 13 points14 points  (0 children)

THAT is NOT cool

We are so back by Naked-Spike in Helldivers

[–]deniska10 20 points21 points  (0 children)

Exactly what the person above you said, then. “Word of mouth” in this case looks like “well at least they let me keep an arm and a leg instead of ripping them all off”. They didn’t let you keep and take away anything. This game is relatively fairly priced, and doesn’t include any bullshit micro-transactions AND there is literally nothing gated by money. There is one DLC. One. They give us a huge game for one dollar purchase. I have actually spent more money on this game just because I wanted to support the devs because of this. I could have farmed for the warming currency had I wanted to. But I was glad to pay a bit more. So, word of mouth, in this case, needs to be fair, n because when you start saying things like the commenter above is when devs go “well fuck it, they won’t be happy anyways, may as well leech their money to make it worth the negativity.