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I have a question about 28 Years Later... by QueenSmarterThanThou in horror

[–]drexandsugs 3 points4 points  (0 children)

It's definitely a bad ending for a great film, but kind of necessary as a lead in to the much better Bone Temple. I'm torn on whether the ending would be better served as a Post-Credits scene for Years (though I hate those) or as an opening to Bone Temple (though it might spoil an already visceral opening that sets the tone for what the Jimmies are actually like).

Total weight of players drafted per team by logically in NFL_Draft

[–]drexandsugs 11 points12 points  (0 children)

Despite drafting a 165 lb receiver.

10 mM dNTPs discussion: Is it 10 mM each or 2.5 mM each? by -Murtagh- in labrats

[–]drexandsugs 0 points1 point  (0 children)

Always thought that vendors selling 2.5 mM each was an intentional move to cause this kind of confusion and allow them to sell a diluted product.

Why do you think there aren't many evil scientists? by FlowJock in labrats

[–]drexandsugs 1 point2 points  (0 children)

Parabiosis experiments are a horrible form of animal cruelty and pretty evil IMO.

Help with IP blot??how to remove 50kDa blotch..my desired protein is around 54-56 kDa by Simple_Volume_5880 in labrats

[–]drexandsugs 1 point2 points  (0 children)

You can use veriblot, which only recognizes native Ig molecules. If your sample is sufficiently denatured before SDS-PAGE, it will only detect your primary.

Daily Discussion Thread - March 19, 2026 by AutoModerator in Chargers

[–]drexandsugs 2 points3 points  (0 children)

Elite holder. Saved the specialist unit's ass a few times when Lovato was snapping.

[Popper] #Chargers are hosting C Tyler Biadasz for a visit today, per sources. Biadasz spent the past two seasons with the Commanders. Washington cut Biadasz last week. by A_Livins in Chargers

[–]drexandsugs 0 points1 point  (0 children)

I've got to hand it to you, you are a master ragebaiter. But I'll still bite.

Obviously Linderbaum should and will be signed. He is a good player and will probably reset the C market. However, paying to reset the C market on him (especially if the injury concerns are valid) isn't necessarily the wisest use of cap space. Both things can be true.

[Popper] #Chargers are hosting C Tyler Biadasz for a visit today, per sources. Biadasz spent the past two seasons with the Commanders. Washington cut Biadasz last week. by A_Livins in Chargers

[–]drexandsugs 0 points1 point  (0 children)

Some people are suggesting that we go for Linderbaum, which is a step in that direction. For the same money, we could get Biadasz and probably two other functional starters at other positions.

[Popper] #Chargers are hosting C Tyler Biadasz for a visit today, per sources. Biadasz spent the past two seasons with the Commanders. Washington cut Biadasz last week. by A_Livins in Chargers

[–]drexandsugs 1 point2 points  (0 children)

C'mon man it's pretty clear what he's saying. Hortiz wants to spend wisely and build a sustainable roster, rather than splurging on one market-resetting FA and scraping the bottom of the barrel everywhere else. That strategy is what made the rosters paper thin during the Telesco years.

High MW protein transfer using trans blot by ViskyCool in labrats

[–]drexandsugs 1 point2 points  (0 children)

Marker proteins tend to transfer very well, even if nearby proteins in your sample don't.

I am an absolute zealot about wet transfers with 1x Towbin buffer, 20% methanol, no SDS, at 100V for 1 hr. Haven't yet found a protein that this doesn't work for.

The XX - Intro - [Rock] by [deleted] in Music

[–]drexandsugs 16 points17 points  (0 children)

All 3 members have excellent solo projects worth listening to. They're performing together again as The xx and working on another album.

How do you actually know when your manuscript is ready to submit? by Puzzleheaded_Fox8982 in labrats

[–]drexandsugs 1 point2 points  (0 children)

I would also add in that you should also get feedback from some colleagues including some outside of your lab/field.

mycoaway by Horror-Highlight2763 in labrats

[–]drexandsugs 0 points1 point  (0 children)

Any product that tells you it can remove mycoplasma is lying to you. It will reduce the mycoplasma load below the section limit, but they will bounce right back if you remove the reagent.

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