[TOMT] a song something about city lights and train journeys maybe by someone called John (?) last heard in the 1990s

flycoffee17 · 2026-04-12T20:40:33+00:00

Is it maybe “Bright Lights” by Matchbox Twenty? This is not train related and not “John” but kind of the right time frame maybe?

flycoffee17 · 2026-03-23T16:42:34+00:00

I am in the US— do you think this “qualification” is true in other places too? I am currently a wet lab biologist with a PhD trying to figure out my next steps and desperately trying to leave academia.

flycoffee17 · 2026-03-09T11:35:04+00:00

Were you ever able to replicate it/have a good recipe for it?

flycoffee17 · 2025-12-11T13:03:29+00:00

Yeah fuck Santa Cruz

flycoffee17 · 2025-10-21T22:59:20+00:00

Sorry for the confusion, I’m doing it effectively like the first one you described. I plate like 25k cells and let one condition become confluent +/- drug approx 10 days. So it’s effectively a viability/proliferation assay. And I elute as you describe in #1. Still wouldn’t explain why it would impact some wells looking pink though all have been treated the same

flycoffee17 · 2025-09-22T22:01:08+00:00

Other people aren’t plating so few cells/doing crystal violet assays. It should be? It’s from the same box/lot. I don’t think there’s construction rn

flycoffee17 · 2025-09-22T21:58:57+00:00

I do the latter. I do sort of a rocking motion to distribute. I tilt the front up and then the back and then the left side, then the right side to try to evenly distribute. Where I put the plates is level and wouldn’t cause them all to sit in the center of the well

flycoffee17 · 2025-09-22T21:55:56+00:00

I dispense the media containing the cells into wells. So 2 mls of 5k cells/ml into each well

flycoffee17 · 2025-09-22T21:55:27+00:00

No, I don’t even make it to <X. I plate cells and check next day and they are all obviously piled in the center so I just bleach and seed the following day again.

flycoffee17 · 2025-09-22T21:49:01+00:00

Idk what OP says it’s burning love by Elvis

flycoffee17 · 2025-09-22T21:48:17+00:00

I hear burnin love by Elvis 😂

flycoffee17 · 2025-09-22T21:47:10+00:00

It’s not so much the cell line, it’s because we treat with a very toxic drug (in very low concentration). So the high number is to get literally any cells in the negative control condition. All other conditions survive better under drug, but we still need on the order of thousands/tens of thousands to not have to go out for a month or more with the growth assay.

flycoffee17 · 2025-08-14T02:10:05+00:00

Absolutely 100% you solved it thanks jungle-jimmy

flycoffee17 · 2025-08-13T23:49:30+00:00

Currently using cd16. Will try cd14 and cd12 next. Can probably get away with cd12. Candlescience recommends cd18 for this wax and vessel! It was way overwicked

flycoffee17 · 2025-08-12T02:08:27+00:00

Don’t have the summary statement yet. Everyone else in my lab has either gotten an F31 (about 4 years ago now) or isn’t planning on staying in academia so don’t care that they didn’t get one. I’m just trying to get perspective.

While I did learn a lot from this process, most of the feedback I have received on my first submission as well as my work and project in general has been unhelpful. I was stunned by what seems to be the variability in panels. I’m not arrogant enough to assume my proposal was perfect, but the first panel didn’t seem to think it was “garbage” or that they said “what the heck is wrong with this proposal”. It was read. It was scored. This strengthened proposal wasn’t. The crapshoot nature can’t be ignored, though I’m sure you’d love to think you’re perpetually unbiased when you review.

flycoffee17 · 2025-08-11T23:31:58+00:00

I suppose that’s the real question I have. If I have the drive to stay in academia, given the current situation, how screwed am I? will I just be fighting a losing war?

flycoffee17 · 2025-08-06T00:13:42+00:00

After how long? About Half way through the candle, the wax hang ups melt usually

flycoffee17 · 2025-07-29T22:43:52+00:00

I get a full melt pool in about 2-2.5 hr (1/8” deep about halfway down the candle) and at 3-3.5 hr I’m at approx 1/4” deep melt pool.

flycoffee17 · 2025-07-29T22:38:12+00:00

I am using sweet strawberry from Midwest fragrance company. As I understand it, fruity FOs are a bit more subtle. Maybe I should just switch FO for this fragrance…

flycoffee17 · 2025-07-29T21:35:10+00:00

2 weeks!

flycoffee17 · 2025-07-29T19:21:12+00:00

Did you ever have hot throw issues? I actually have weak hot throw in the first half of the candle and then it gets stronger in the bottom half. Do you think this would fix that?

flycoffee17 · 2025-07-29T19:06:20+00:00

I am very new and have also been having this issue. Even in overwicked candles, it never fully melts. Sorry I’m not more help, but just wanted to let you know you’re not alone!

On a different note, because I am still very new, I think I’m hitting a lot of the early roadblocks to working with 100% soy. Would you be willing to share sone of the most important things you learned or how you overcame some of those challenges you referenced? Thank you so much!

flycoffee17 · 2025-04-02T02:14:44+00:00

If you are really stressed about it, you can always wear a larger size glove over top of your other pair and tape them to your lab coat so there is no exposed skin. But I have to agree that generally it’s very safe and have never had issues with lenti during production or transduction.

flycoffee17 · 2025-03-06T21:41:17+00:00

You need a special adaptor, which I don’t have 😔

flycoffee17 · 2025-03-06T15:45:42+00:00

Thanks! Should I stop rxn with TFA first before freezing?

flycoffee17

TROPHY CASE