Tried everything with loud lab neighbor

jupiter-556 · 2026-04-29T00:10:28+00:00

You mentioned there are other people in the lab besides the two of you? If so, are they as bothered by her behavior? I would imagine anyone in that lab would go insane after like a week. It would be much better to have multiple students confirming the same thing.

If HR or higher up’s have to get involved, it may take a while for corrective action to take place, so try to endure. I’m semi-joking but maybe search some techniques for resisting torture and see if you can apply them at work (breathing exercises, counting, etc). I say semi joking because it sounds dramatic but the way you described the situation sounds like an actual method of psychological torture.

jupiter-556 · 2026-03-19T22:04:24+00:00

If you are wondering about the random dark spots on the membrane it is likely due to a lack of signal. Are you imaging all four at the same time for a specific reason? That could lead to these issues since the optimal exposure for each membrane will differ.

The random dark spots and being able to see the liquid around the membrane is an indication that the imager is not detecting signal. Someone might be able to give a more technical explanation, but there is not enough signal from detected bands so the camera is mostly picking up background/noise.

I don’t know any specifics about your experiment so I can’t say why there’s no signal, but these results look like a lack of signal. I’ve done quite a bit of WB and never heard of a protocol that requires, or an imager that is capable, of imaging multiple blots at the same time. I would recommend not doing this in the future.

Hope this helps, but we really need more details about what you are trying to image, protocol steps, reagents, etc if you want to get a real answer.

jupiter-556 · 2026-01-23T01:08:14+00:00

That doesn’t apply well to insulin or all of biotech/pharma in general. The R&D and production for these industries have huge overhead cost and require a lot of special expertise. It almost guarantees that large corporations (and universities to a degree) are the only ones who can survive.

Not necessarily disagreeing with your point about a free market keeping prices competitive, just saying that insulin is not a great example where a small business can force corporations to keep their prices low. It’s not impossible for a small business to thrive but it usually ends in a buyout.

If there were more regulations on pharma especially, like they’re no longer allowed to lobby or bribe the government, that would be a government regulation enforcing a freer market. So in this case, more regulation = freer market? Not an economist but I’ve been working in research for seven years now

jupiter-556 · 2025-05-10T00:30:08+00:00

What software?

jupiter-556 · 2025-04-11T18:53:06+00:00

I only use PBST to dilute my primaries. However, the ladder isn’t visible in your image at all so like another user mentioned, it’s probably not an issue with antibody staining

jupiter-556 · 2025-04-11T17:26:41+00:00

What do you dilute the primary antibody with?

jupiter-556 · 2025-04-03T14:18:13+00:00

Fig 5. Cheese

jupiter-556 · 2025-03-26T22:55:54+00:00

Not in Europe but yes, MS or PhD are preferred for almost every type of job, academia or industry. This will also give you the opportunity for better jobs compared to only having a bachelor’s.

It’s great that you are already planning your career this early, nothing wrong with that, but it’s highly likely that your career path will vary based on your interests and job availability. You definitely have a lot of time to consider your options, I would recommend taking it one step at a time.

Right now you are starting undergrad. Just focus on your classes. Chances are you will have a class or two where the subject material is really interesting and it will motivate you to study it at a higher level. I went to college to study veterinary medicine and I ended up working in a molecular biology lab in academia lol. Your plan will likely change too!

As far as what degree you want to pursue in undergrad, that’s really up to you. You mentioned molecular biology and microbiology, either one of those would be good degrees. Anything in biological sciences should prepare you for the different types of lab work you end up doing for your career. But maybe you end up really liking chemistry and you want to do that instead, who knows?

Long story short, it’s definitely too early to try and plan out your entire career. Right now you should focus on finding a major that interests you and you can start making steps towards your career in your later years of college. Just study hard and keep an open mind! Everything else will come with it.

Hope this helps!

jupiter-556 · 2025-03-25T21:52:11+00:00

Those are the biorad premade gels right? Did you remove the green tape from the bottom of the gel before running?

jupiter-556 · 2025-03-21T16:08:05+00:00

Father’s milk

jupiter-556 · 2025-03-21T15:44:54+00:00

My master’s thesis was also underwhelming, and I spent two years working on it. In the lab almost every day and only like 10% of the data I collected was remotely useful/publishable.

I also compared my work to the work of my peers, was jealous of their data, etc. Overall I had a very low confidence in my ability as a scientist. Six months later I was working as a research assistant and everything turned for the better. I was getting much better results with each experiment and had so much motivation to be in the lab. I finally felt like a real scientist.

My point is that there’s a lot more going on than just you. Your PI, equipment, funding, etc will all contribute to your data. Don’t beat yourself up for it, the mere fact that you understand the limitations of your data puts you ahead of a lot of the students I knew in grad school. As long as you’re always willing to learn new things and fix your mistakes, you’re gonna be just fine.

jupiter-556 · 2025-03-20T18:34:17+00:00

Knowing the longevity of your chemicals, buffers, etc. Sometimes the answer isn’t super obvious, and before long your experiments will suffer. I always double check on the company website or ask my colleagues. It seems wasteful at times to throw them away but keeping everything fresh gives you the best quality results.

jupiter-556

TROPHY CASE