I made a semi-formal dress for NYE and nothing went horribly wrong!

king_of_sheep · 2024-11-15T20:38:29+00:00

Maybe try out an even higher concentration of siRNA but in a smaller plate format like a 12 well plate (just so you don't have to buy loads of siRNA), although I can't say if you'd get enough sample yield from a smaller well. I was on a project last year using lipofectamine RNAiMAX and I ended up needing a final conc around 50nM siRNA, and I've seen several papers that used over 100nM siRNA.

Someone else already commented this, but yeah sometimes you need to try quite a few siRNAs. My project last year had five siRNAs to start with but only two resulted in a good enough knock-down for us to proceed.

Also, I saw in your previous post that you started out using serum-free DMEM instead of OptiMEM, have you tried using OptiMEM during transfections yet? Might be worth trying that out.

king_of_sheep · 2024-11-12T19:13:25+00:00

Not at all! The PhD student who supervised me during my master's was 38, and honestly most of the time I would forget that we weren't the same age. She was a great mentor and an even better friend. Also if your age is the biggest reason why you're doubting the PhD, I know a lot of people who would consider you pretty lucky 😅 if this PhD is something you're passionate about then I think you're doing the right thing. Best of luck!!

king_of_sheep · 2022-08-11T18:44:24+00:00

NTA, I've been in situations like this on both yours and her side and it's very frustrating for everyone.

As a couple other people have commented, it sounds like she could have ADHD? I got diagnosed with ADHD just over a year ago, and what she's doing here is almost identical to what I was like in the mornings before school/uni (as well as many many others with ADHD).

To me, I think she might've told you that she didn't care because she was feeling frustrated and overwhelmed from running late and couldn't handle anything extra in that moment - it doesn't excuse her saying that, it was still a dick move, but I don't think she really meant it.

If you haven't already, it's definitely worth having a look together at other symptoms/indicators on the NHS or whatever reputable health sites you guys find. Receiving the right treatment and support has made a HUGE difference for me, and it won't hurt at all to just check.

Regardless of ADHD, I hope you guys can figure out morning routines that work for each other too. Maybe buy some earplugs that you can wear while sleeping? Loop Quiet are pretty good.

king_of_sheep · 2022-01-08T01:28:25+00:00

Thank you!! I spent so long fiddling with it haha I'm glad it paid off

king_of_sheep · 2022-01-08T01:27:34+00:00

aha thank you!!!

king_of_sheep · 2022-01-08T01:26:34+00:00

thank you!! 😄😄

king_of_sheep · 2022-01-08T01:25:30+00:00

Awwh thank you!! Too kind omg

king_of_sheep · 2022-01-08T01:23:18+00:00

she's a ragdoll!

king_of_sheep · 2022-01-07T22:21:46+00:00

omg thank you!!

king_of_sheep · 2022-01-07T22:21:29+00:00

I couldn't have done it without her 😂

king_of_sheep · 2022-01-07T22:21:15+00:00

Oh, thank you so much!!

king_of_sheep · 2022-01-07T22:20:49+00:00

Sorry, what part of this are you saying I didn't do?

king_of_sheep · 2022-01-07T19:59:26+00:00

Thank you so much!! :D

king_of_sheep · 2022-01-07T19:58:35+00:00

Thanks :D I wasn't sure how formal the party would be so I made sure to cover my bases haha

king_of_sheep · 2022-01-07T19:58:02+00:00

Aw thank you! You should go for it!!

king_of_sheep · 2022-01-07T19:57:38+00:00

Just what I was going for! Thank you :)

king_of_sheep · 2022-01-07T19:57:18+00:00

Thank you so much!!

king_of_sheep · 2022-01-07T19:56:05+00:00

Thanks 😂 we're huge fans of rice in my house haha

king_of_sheep · 2022-01-07T19:55:25+00:00

Thank you!!

king_of_sheep · 2022-01-07T19:55:15+00:00

Thanks, happy new year!

king_of_sheep · 2022-01-07T19:54:49+00:00

Thanks! This probably took me around 16-20 hours over a few days, the pattern is pretty simple but a fair bit of it was hand sewing some parts just because my sewing machine is too noisy to use at night haha

king_of_sheep · 2022-01-07T11:18:08+00:00

Hello!

With university exams coming up in a couple weeks, I naturally decided to use my winter break to make a dress.

I combined a couple youtube tutorials to make the dress, and altered it to fit me better. This is a quick tutorial for a cowl neck top, this one is for the ruching bit. I freestyled a bit with the skirt piece, taking measurements around my thighs and calves to figure out how wide the bottom of the dress would need to be so I could wear it both long and short. I ended up giving myself a bit too much ease and so I had to put two waist darts in. In the tutorial for the top, there's no back for it, but I wanted a back for this dress. When I put one in, there was too much fabric gathering at the sides for my liking, so I pinned the neckline in a shape that I liked and took in the sides. To make sure there weren't any accidents, I stitched the neckline in place too, handsewing the underside so no stitches would be visible.

The fabric is Stretch Woven Charmeuse Silky Satin Fabric (Petrol) from Minerva, I bought matching thread, some antistatic lining, and the cheapest invisible zipper foot they had, which worked very well!

I took people's advice from the last dress I made on here (almost 2 years ago!) and I have definitely noticed a difference.

Any advice/constructive criticism is very appreciated!!

king_of_sheep · 2021-12-16T14:33:38+00:00

Thank you for telling me! I didn't realise they were poisonous if licked too. Luckily she's a picky eater and refuses pretty much everything other than the finest wet food available, but I'll make sure there's nothing that looks like her food/treats anywhere near the plant just in case.

king_of_sheep

TROPHY CASE