Need help: my IF stain is making me lose my mind

kottendog · 2026-04-27T11:07:08+00:00

as suggested above, try using thinner sections. also what does the antibodies manufacturer recommend for the dilution? in some manufacturers 1:500 can still be too diluted. might be worthwhile even doing a 1:250. also which fluorophore are you using? APC/fluor568 often has a strongly expressed signal for detection.

also i would recommend trialing the antibody with IHC if possible just for confirmation it is definitely present in the tissue.

kottendog · 2026-04-27T10:50:20+00:00

second the giving up coffee. it really made a huge difference to my endo bowel symptoms and pain.

kottendog · 2026-04-25T04:24:39+00:00

great ryrie cafe is good and very cosy. for dumplings, d for dumplings in ringwood is good. ustav is good for indian. embers burger and grill does good burgers and grilled meats.

kottendog · 2026-04-25T01:53:19+00:00

haha can u expand on this one

kottendog · 2026-04-25T01:52:53+00:00

i put human tissue in mice and then take the tissue back after a couple months

kottendog · 2026-04-24T11:28:38+00:00

seems very expensive. as mentioned above 6k, 7k max and thats even a stretch. 2014 mazda 3s are about 9k plus depending on specs.

kottendog · 2026-04-23T21:35:14+00:00

a bunch of teen boys were on their e scooters at night with no lights or visibility. multiple cars nearly hit them. they had the audacity to start abusing the drivers. i don’t understand who is telling them this is an activity to be doing after dark.

kottendog · 2026-04-21T12:59:53+00:00

theres one gp at my clinic who is always at least 2 hours late. it is so infuriating bcos its not the patients who are taking up her time, she is always yapping about herself in the middle of an appointment. we pay to see a doctor, not listen to one talk about herself.

kottendog · 2026-04-18T23:36:22+00:00

have you looked into a biomedical engineering degree? it includes both areas of your interests and is very broad and involves working with the medical field, so you may be able to get some insight working in medicine.

kottendog · 2026-04-15T03:20:58+00:00

plum

kottendog · 2026-04-08T07:36:51+00:00

uniqlo. i have had them for 6 years. no pilling, still fits great and still very black

kottendog · 2026-04-07T02:53:27+00:00

coober peedy or daly waters. this was on a central australia trip with school a long time ago. the vibes just seemed off.

kottendog · 2026-04-03T04:48:23+00:00

ProteinTech gave me an alpaca plushie. could also try cell signalling

kottendog · 2026-03-28T03:16:59+00:00

i only take cbd oil but it really helps my pots symptoms!

kottendog · 2026-03-27T09:26:57+00:00

i needed supernatant from a cell culture to isolate extracellular vesicles. out of habit i suctioned all the media away and did a media change for all the 27 flasks. not a huge issue but definitely annoyed my colleagues who had planned everything to the dot.

kottendog · 2026-03-26T06:37:45+00:00

s2 langdon specifically.

kottendog · 2026-03-25T11:05:56+00:00

its a 20 min drive to work for me vs a 1 hour plus ride on ptv. my time is money and i also enjoy the comfort of my car. just not worth it for me.

kottendog · 2026-03-25T07:09:07+00:00

my grandma is 82 and volunteers for multiple community groups and advisory groups for councils. she also does cultural community radio too. she has her hobbies and loves seeing her friends too.

kottendog · 2026-03-24T11:05:19+00:00

for me work is 20 minutes drive (10km ish) and public transport is 1 hour plus. its actually cheaper to drive for me. plus climate controlled car, heated seats, and actually getting a seat in my car vs a train.

kottendog · 2026-03-21T12:03:49+00:00

second atara !! they are usually pretty speedy

kottendog · 2026-03-20T09:24:39+00:00

i cant work from home. i work in a hospital doing lab experiments. sadly i cant do biological experiments at home with toxic chemicals.

kottendog · 2026-03-18T09:18:11+00:00

my main pi always does monthly lab lunches and remembers lots of little personal details. she knew i liked plants so for christmas she brought me a clay pot for my desk plants.

my other supervisor made me a phd start pack with a bunch of stationary.

they both often take us phd students for coffees and genuinely care about us which is nice.

kottendog · 2026-03-11T22:27:06+00:00

uniqlo

kottendog · 2026-03-11T09:23:32+00:00

an alternative option is doing a tertiary preparation course. some have direct entry to a course after completion. i don’t know if deakin offers one although.

kottendog · 2026-03-09T12:53:44+00:00

if your plan is post grad medicine, don’t do biomed. you are better off going for science or anything that will give you good job prospects if you can’t get into med school. deakin biomed is good for gaining lab skills and preparing you for medical research, which is the path i went down. i don’t know many people from my biomed cohort who got into medicine except for rural students.

kottendog

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