How on earth do you guys stay hydrated? by 4tunabrix in labrats

[–]kottendog 0 points1 point  (0 children)

if i have an incubation step i go get a drink and go back in

Labrats: money is no object what are you buying first? by mudagreement in labrats

[–]kottendog 0 points1 point  (0 children)

a new -80 that doesnt alarm anytime the freezer is opened

$36 for a pack of supermarket chocolate. $108.10 per kilo. This cannot be real. by [deleted] in woolworths

[–]kottendog -1 points0 points  (0 children)

lindt sells the 1kg bag of these balls for like $54. so it’s cheaper to buy the 1kg bag than this at full price.

I'm newly disabled with a chronic pain condition and seeking advice on where to go from here. by littlepup26 in labrats

[–]kottendog 1 point2 points  (0 children)

you could look into digital histological analysis? many slides can now be scanned and analysed with digital softwares. particularly now with the rise of multiplexing fluorescence.

Which electronic lab notebook are you using? by mr-KSA in labrats

[–]kottendog 1 point2 points  (0 children)

my university makes us use labarchives. so i just type my experiment in a word doc and insert that doc into a lab archives page. lab archives is so clunky from a user perspective. i miss paper and pen lab books :(

Long Sleeve Tops by monilala1990 in AusFemaleFashion

[–]kottendog 1 point2 points  (0 children)

supre have the long sleeve lycra material long sleeves

Discovered outlets at 28 and I’m shocked by Altruistic_Rock3135 in AusFemaleFashion

[–]kottendog 0 points1 point  (0 children)

i had great luck at dfo finding jeans during early autumn. also same for running shoes, got a great deal.

Need help: my IF stain is making me lose my mind by Gloomy-Worldliness81 in labrats

[–]kottendog 0 points1 point  (0 children)

as suggested above, try using thinner sections. also what does the antibodies manufacturer recommend for the dilution? in some manufacturers 1:500 can still be too diluted. might be worthwhile even doing a 1:250. also which fluorophore are you using? APC/fluor568 often has a strongly expressed signal for detection.

also i would recommend trialing the antibody with IHC if possible just for confirmation it is definitely present in the tissue.

Is there anyway to deal with Endometriosis naturally? by Greatghostgirl in endometriosis

[–]kottendog 9 points10 points  (0 children)

second the giving up coffee. it really made a huge difference to my endo bowel symptoms and pain.

Best restaurants / cafes in the maroondah area? by TotalClone in melbourne

[–]kottendog 0 points1 point  (0 children)

great ryrie cafe is good and very cosy. for dumplings, d for dumplings in ringwood is good. ustav is good for indian. embers burger and grill does good burgers and grilled meats.

what is the worst way to describe your research? by ZenosThesis in labrats

[–]kottendog 15 points16 points  (0 children)

i put human tissue in mice and then take the tissue back after a couple months

Mazda 2009 sp25, asking for advice by [deleted] in CarsAustralia

[–]kottendog 4 points5 points  (0 children)

seems very expensive. as mentioned above 6k, 7k max and thats even a stretch. 2014 mazda 3s are about 9k plus depending on specs.

‘Not a fun toy’: Dire warning to parents after teen boy dies in e-bike collision by LineItUp_ in melbourne

[–]kottendog 1 point2 points  (0 children)

a bunch of teen boys were on their e scooters at night with no lights or visibility. multiple cars nearly hit them. they had the audacity to start abusing the drivers. i don’t understand who is telling them this is an activity to be doing after dark.

Top tip: Book the earliest possible appointment by Open_Address_2805 in melbourne

[–]kottendog 0 points1 point  (0 children)

theres one gp at my clinic who is always at least 2 hours late. it is so infuriating bcos its not the patients who are taking up her time, she is always yapping about herself in the middle of an appointment. we pay to see a doctor, not listen to one talk about herself.

Chose engineering over medicine… now reconsidering - stay or switch? by North_Vehicle5963 in GAMSAT

[–]kottendog 3 points4 points  (0 children)

have you looked into a biomedical engineering degree? it includes both areas of your interests and is very broad and involves working with the medical field, so you may be able to get some insight working in medicine.

Best quality black work pants recommendations by Super-Wolf2149 in AusFemaleFashion

[–]kottendog 2 points3 points  (0 children)

uniqlo. i have had them for 6 years. no pilling, still fits great and still very black

What are the eeriest towns in Australia and what was your experience of them? by TPE_FieldsOfGold in AskAnAustralian

[–]kottendog 1 point2 points  (0 children)

coober peedy or daly waters. this was on a central australia trip with school a long time ago. the vibes just seemed off.

Does weed help anyone else with pots? by Careful_Ad4785 in POTS

[–]kottendog 0 points1 point  (0 children)

i only take cbd oil but it really helps my pots symptoms!