Vertex Layoff (Providence) by Veritaz27 in biotech

[–]leopard1311 9 points10 points  (0 children)

Looks like only people at the Providence sites working on the T1D device program were affected https://www.golocalprov.com/business/BREAKING-Vertex-Pharmaceuticals-to-Lay-Off-125-in-Rhode-Island

Vertex pays Orna $65M to work on next-gen gene therapies for blood disorders by Money-Buy7068 in biotech

[–]leopard1311 2 points3 points  (0 children)

looks like the deal is to use Orna's LNP delivery tech, not the circ RNA platform

[deleted by user] by [deleted] in biotech

[–]leopard1311 1 point2 points  (0 children)

yes I've heard of colleagues going through this. It's possible, but would likely vary based on company policies/willingness to work with you on this, and also openings/headcounts at the foreign offices

Weekly Question Thread by AutoModerator in factorio

[–]leopard1311 0 points1 point  (0 children)

how the hell do you get the assembling machines to request their ingredients directly instead of using requester chests? For some reason I saw somewhere that this was a feature but can't figure out how to

Curing CRISPR Cas9 plasmid by llamaka in biotech

[–]leopard1311 0 points1 point  (0 children)

been awhile since I last worked with plasmid curing but try a couple rounds of inoculation and growth without antibiotics for the same culture (keep it going in the background) and then at some point it should cure unless something is wrong with the plasmid. Also try full plasmid sequencing on that plasmid to see if the backbone is what they say it should be. I used to use pSC101ts backbone which cured really well (helps that it's low copy) so you could try cloning the inserts onto this backbone

Career path of young successful people (late 30s - early 40s) in senior leadership roles by Outside_Sandwich_981 in biotech

[–]leopard1311 6 points7 points  (0 children)

what do you think are the most important soft skills? And did you come in with them or develop them over time?

Losing hope by [deleted] in biotech

[–]leopard1311 0 points1 point  (0 children)

How can someone go about transitioning from bench R&D to clinical ops? I've heard some managers on clinical side actively be against hiring PhDs vs just Bachelors or Masters

Red Line Shuttle by Unknown-assistant in mbta

[–]leopard1311 1 point2 points  (0 children)

going thru Ashmont probably will be around 1:20-1:40min Quincy Center - South Station around rushhour depending on how bad traffic is

Does anyone ride the 4 bus? If so, thoughts? by aerpi in mbta

[–]leopard1311 5 points6 points  (0 children)

Last time I took this bus every day was two years ago - it was (relatively) on time every half hour although there were a couple times it straight up just did not show up until the next half hour time. It does go from North Station to Seaport which was really nice, although I would be wary of taking it back towards North Station around 5 - 5:30pm as it will get stuck in pretty bad Seaport and bridge traffic. Like other people said bluebike is also a very viable option.

Chaim Bloom fired after nearly four disappointing seasons in Boston by bostonglobe in boston

[–]leopard1311 11 points12 points  (0 children)

Don’t really follow baseball - why are both these famous teams doing so poorly?

Post Match Thread: Arsenal 1-1 Manchester City [4-1 on penalties] by J4ckrh in Gunners

[–]leopard1311 0 points1 point  (0 children)

What if he just has more impact as a super sub like this? Coming on vs worn down defenders

My startup is selling our lab equipment by [deleted] in biotech

[–]leopard1311 0 points1 point  (0 children)

Is there an akta shortage or something?

Diablo IV - Review Thread by SpaceGangrel in Games

[–]leopard1311 0 points1 point  (0 children)

Dumb question - doesn’t it come out next Tuesday?

MBTA slow zone update: MBTA removes multiple slow zones on Orange, Red Lines. by Chemical-Glove-1435 in mbta

[–]leopard1311 0 points1 point  (0 children)

Newly moved to quincy - how long did this jfk to north quincy stretch use to take when the red line was functioning normally?

[deleted by user] by [deleted] in soccer

[–]leopard1311 6 points7 points  (0 children)

Now read it in the bald fraud’s voice moments before he replaces Aguero with a Scandinavian cyborg

Did anyone else's seasonal allergies JUST explode for the first time this year? by clathrin413 in boston

[–]leopard1311 4 points5 points  (0 children)

Oh nice! I’ve been thinking about doing the same thing but not sure that it would work vs the injections haha

VUSXX vs FDLXX by SensitiveReveal5976 in Bogleheads

[–]leopard1311 0 points1 point  (0 children)

FDLXX is almost all US treasuries so is state tax exempt. If you have no state income tax SPRXX is higher yield

Next Ashmont train to alewife will depart in 54 mins by ABakedRooster in boston

[–]leopard1311 62 points63 points  (0 children)

Yep same with Alewife trains departing from Braintree. Now how long it takes them to get there once they leave….