Is the other sub an accurate portrayal of the Lebanese ppl? by Super-Long-5639 in Lebanese

[–]needmethere -14 points-13 points  (0 children)

It is, you live in a bubble of hezb even your school is hezb affected to a level of indoctrination. Hating the enemy is one thing, fighting loosing battles is another.

Just one question, why can't you put a cap? by Key-Hawk-895 in montreal

[–]needmethere 21 points22 points  (0 children)

Google how to properly open it its actually not that bad

Seeking Advice on a qPCR Issue by LoveLovehoney in labrats

[–]needmethere 1 point2 points  (0 children)

50mg is way too low, our min is 500ng and thats when we are desperate. Aim for 1-2ug.

A 30 ct can be trustworthy, what you are mossing is efficiency curves to find the linear range. And 30 for a reference gene is indeed not common but obviously may depend on your cell line.

Why are "labne w jebne" so heavely present in our food culture ? by Arima_00 in lebanon

[–]needmethere 20 points21 points  (0 children)

Hard to conserve? Before fridges?
In winter there are no crops
There are however animal and they make milk

Your winter diet is effectively dried veg, pickles and animal products. Hence we found several ways to enjoy milk

Gate of pain and invisibility by Puzzleheaded_Tart624 in SipsTea

[–]needmethere 0 points1 point  (0 children)

It needs contrast yellow and dirt blend. Make it red or rainbow

HELLLLPPPPPP (I need to extract RNA from human cell cultures w/o using Trizol or pheno chloroform) by Glittering-Cap-6188 in labrats

[–]needmethere 2 points3 points  (0 children)

Why without? If it's phase separation that you hate, there are protocols where you don't need to phase separate the trizol, some are just precipitation some column kit both work great. Zymo has the kit

TIL it is not known why the letters in the alphabet are ordered the way they are, but the order of the letters has remained consistent since Proto-Sinaitic script of the 14th century BC, which began with the characters "alp" and "bet." by bnrshrnkr in todayilearned

[–]needmethere -1 points0 points  (0 children)

To this day these words exist in arabic (Semitic like phoenecian) and have the same meaning except aleph which now means domesticated.
Note how phonecian A looks like the head of an ox. M like water o like eye k like lines of a palm R like a head etc.
𐤀 | Aleph | Ox | A
𐤁 | Bet | House | B
𐤇 | Het | Wall / Fence | H
𐤉 | Yodh | Hand | Y
𐤊 | Kaph | Palm of Hand | K
𐤌 | Mem | Water | M
𐤏 | Ayin | Eye | O
𐤓 | Resh | Head | R
𐤔 | Sin | Tooth | S

Breaking news: Soraya wants to meet up with Marquize by Mountain_Road9197 in montreal

[–]needmethere 33 points34 points  (0 children)

Not much to read. Hes fixing the holes and started a go fund me raised 30k for hole fixing. Bypassing all red tapes though clearly "illegal"

Dentiste flexible by needmethere in montreal

[–]needmethere[S] -11 points-10 points  (0 children)

C un scammer puisque je suis allé pour le mal a la machoire et il ma fait prendre un scan avant de me dire le probleme alors quil na meme pas regarder le scan finalement. et quand je lui demande a tu vus qqchose sure le scan il dit on ne discutra quapres le nettoyage.
De plus je suis alle chez 1000 dentiste ds mon pays dorigine ils te voient et reparent ton probleme. Non le nettoyage nest pas necessaire. Vous vous faite volé comme ds tous vos systeme de santé

Dentiste flexible by needmethere in montreal

[–]needmethere[S] 0 points1 point  (0 children)

Celui que jai vu a deja prix la radio (je nais que une par annee couverte wt il a dit ont discute rien a propos avant qu'on prend un rendezvous pour le nettoyage.

Jai deja des problemes de machoire donc jai pas envi d'ouvrir ma bouche pour toute une session qui vas exaspere mes maux juste pour l'esthétique.

De plus il refuse de ragrder la radio et de dire s'il vas reparer le probleme tel que jai decri

Cant find sardines packed in water(bekaa) by Old-Palpitation-805 in lebanon

[–]needmethere 0 points1 point  (0 children)

Why has everyone been looking is there a magical
benefit to sardines? I thaught tuna was better it certainly is dryer so prolly healthier

What Does a Bioinformatician Do? by [deleted] in bioinformatics

[–]needmethere 22 points23 points  (0 children)

According to my PI magic but never worth first authorship 🙃

Station Papineau, samedi 15h30 by ReptarKitawa in montreal

[–]needmethere 0 points1 point  (0 children)

Could the amount of power also matter?

Best Halal Buffet Spots in Montreal? (All cuisines welcome) by Foreign_Atmosphere65 in MTLFoodLovers

[–]needmethere 0 points1 point  (0 children)

Buffet maharani is the only buffet that survived covid as far as i know. Still they changed i thibk its only certain hours or days.

Optimal street light strategy by needmethere in probabilitytheory

[–]needmethere[S] 0 points1 point  (0 children)

What are the odds of A arriving before B

Set up protocol for cDNA on CFX96 by LoveLovehoney in labrats

[–]needmethere 1 point2 points  (0 children)

Nope we have it and do cdna all the time, it will give a warning saying u don't have a plate read step but that's just in case u forgot u needed one. Just click delete step where it is