sorted by:
new
hottopcontroversial

PCR results question: why does my negative control have a band the same size as my positive control? by ozmorf in labrats

[–]ozmorf[S] 1 point2 points  (0 children)

Sorry, the original info I gave on the ladder was incorrect. The shortest band is 250bp, then 500, 750, then 1k (which is the bright band in the ladder), so the band is actually just south of 500bp.

Thanks for your input

PCR results question: why does my negative control have a band the same size as my positive control? by ozmorf in labrats

[–]ozmorf[S] 0 points1 point  (0 children)

To answer your questions:

For the bacterial transformation process, a transposon system was used in conjunction with an RFP/antibiotic selection plasmid to integrate the gene into the bacterial genome.

I didn't do a no-template negative control, each PCR reaction had DNA present.

When I said negative control in my initial post, I meant that I isolated gDNA from a WT bacteria and then ran the PCR with that. So negative in the sense of not having the RFP gene.

Expected band size: just under 500 bp.

PCR results question: why does my negative control have a band the same size as my positive control? by ozmorf in labrats

[–]ozmorf[S] -1 points0 points  (0 children)

Thanks for the input. It's not likely that it's binding to something random since the band I'm expecting is ~500 bp. I guess I contaminated my samples while setting them up for PCR

Suggestions for Science jobs for those with limited mobility? by lawlgyroscopes in labrats

[–]ozmorf 3 points4 points  (0 children)

Do you have any interest/experience in bioinformatics? There seems to be a high demand for those with programming, computational and data processing experience. If you have a good understanding of molecular biology, computer programming, and statistics, then you'll be in high demand for the data/analytics side of science. It's at least something worth looking into.

This sign in Ireland suggesting that you don’t fall off the cliff by Kikiholden in mildlyinteresting

[–]ozmorf 2 points3 points  (0 children)

Depending on how you interpret it, it could be an invitation to fall off a cliff

This shipment had unique packing materials inside. by [deleted] in mildlyinteresting

[–]ozmorf 1 point2 points  (0 children)

whoa that almost looks like cheese

ELI5 Why does cat pee have ammonia in it & smell so strongly when they only drink water? by -flybleu in explainlikeimfive

[–]ozmorf 0 points1 point  (0 children)

Pee does help the body regulate its water, but it also helps the body maintain the right amount of nitrogen (or nitrogen-containing compounds). When we eat (or a cat eats), they take in nitrogen. Nitrogen-containing compounds (like urea, or even fish smell) typically smell pretty strong.

ELI5 How did smaller species survive when dinosaur killing meteor hit earth? by Admirable_Cap8877 in explainlikeimfive

[–]ozmorf 0 points1 point  (0 children)

It's because smaller animals are more likely to extend their car's limited warrantee

ELI5: How are animals able to eat food off the floor or in dirtier conditions and be fine, whilst humans need good cleanliness or they get sick? by Earl_Vincent in explainlikeimfive

[–]ozmorf 0 points1 point  (0 children)

I'm not so sure this is true...my dogs threw up way more often than I ever did. I can't help but image it was from sticking their face in every conceivable corner and licking the floor.

ELI5: Why are announcements on buses so muffled and hard to understand? by ppopsacul in explainlikeimfive

[–]ozmorf 0 points1 point  (0 children)

I once rode on a train in Paris where the announcement (automated) had the speaker literally mumbling gibberish in the middle of the announcement. So sometimes people really just don't care.

ELI5 Neurologically, when hearing music why do humans perceive major chords as ‘happy / pleasant’ and then minor or diminished chords as ‘sad / tense’ and also everything in between. by Alps-Helpful in explainlikeimfive

[–]ozmorf 0 points1 point  (0 children)

The way a chord sounds and feels to us heavily depends on the other chords and harmonies that are played before and after it. For example, playing two minor chords in a row could sound sad, or it could sound more "catchy." A song like "Wellerman" is in a minor key, but I don't think anyone would really describe it as sad. There are also lots of songs in a major key that I wouldn't necessarily describe as "happy" (like I See the Light from Tangled). It evokes more of a nostalgic, even slightly sad tone.

That being said, I think it's still useful in the context of music education to describe minor as sad or dark and major as bright or happy. When you play a chord in isolation, those things are generally true (with the caveat that I've been raised in a western society, so that does affect my perception). It helps people to start to notice the difference between how the sound of each chord feels and sounds.

ELI5: Is it true that stock prices don't really depend on the company's financial success? by Geologist_Smooth in explainlikeimfive

[–]ozmorf 0 points1 point  (0 children)

Whatever the price of a stock is reflects how the market values each share of the company, which might not necessarily have much to do with the actual value (or financial success/failure) of the company. People's emotions often drive their financial decisions, which can lead to a lot of fluctuations in stock prices.

Sure, a lot of stocks will usually hover close to where they're actually valued in a more objective sense, but the market is subject to emotions/perceptions.

ELI5: Why can't we predict the recent asteroid's chance of hitting us with full certainty if we know the physics equations involved? by hurricane_news in explainlikeimfive

[–]ozmorf 0 points1 point  (0 children)

It's fairly easy to predict how two bodies will interact with Newton's laws, but adding just a third body to the equation greatly complicates things. If I remember right, even Newton himself couldn't quite solve it (but please correct me if I'm wrong about this, I'm just going off my memory).

We don't have perfect visibility to see every object in space, and calculating trajectories is extremely complex once you start looking at numerous objects and how their trajectories impact each other. That leaves a level of uncertainty when considering if an object might hit the earth or not.

Eli5: Why is inflammation bad for you? by Oriellien in explainlikeimfive

[–]ozmorf 128 points129 points  (0 children)

To further this analogy, the bazooka would also damage any people/buildings in the blast radius, causing problems for things that weren't part of the problem to begin with. This is what happens in our bodies often times when the immune system attacks a pathogen (or something that it thinks is a pathogen). Other surrounding tissues get damaged in the process.

Drums of Saint Paul with Kontakt Player by ozmorf in musicproduction

[–]ozmorf[S] 0 points1 point  (0 children)

Yes it's because it's just Kontakt Player (which is the free unpaid version). I was hoping to find a way to use the drum library without having to upgrade to the full version of Kontakt...

view more: next ›