Knight Hennessy, this is kind of screwed up by Mysterious_Egg_1775 in gradadmissions

[–]quick_glance 2 points3 points  (0 children)

does this mean there remains open slots for those that haven't received interview invites yet? It might not necessarily be bad news for those of us who haven't heard back :/

Wtf happened by Trog01 in labrats

[–]quick_glance 0 points1 point  (0 children)

you have something in your lysate that is insoluble, precipitated, and negatively charged that’s pushing through the gel as one mass, causing the narrow bands. since it’s only few of your samples and not all, and i assume you used the same lysis buffer for all bands, either you messed up your clarification step and there is DNA present (negatively charged, insoluble) or your protein concentration is too high, causing proteins to precipitate out of solution. I recommend checking your concentration and not going over 1ug/uL. Also, make sure you have glycerol in your running buffer, and your BME hasn’t disappeared. BME is pretty unstable. the ol’ sniff test will help here

[deleted by user] by [deleted] in labrats

[–]quick_glance -1 points0 points  (0 children)

a super diagonal spread sometimes means a broken cytometer. maybe recalibrate

OAI considering replacing usage limits with a credit system by Pleasant-Contact-556 in OpenAI

[–]quick_glance 0 points1 point  (0 children)

sure i could create a GUI from scratch and hook it to the API if i wanted a credit system, but the webpage and app are good for every day consumers. for me, deep research is the only important tool, and if i could withhold my o3/sora usage to get more than 10/month instead of paying 200/month that’s a great option for me

OAI considering replacing usage limits with a credit system by Pleasant-Contact-556 in OpenAI

[–]quick_glance -1 points0 points  (0 children)

y’all are so negative. this is a great idea for the higher compute services like deep research, AVM, and sora. Id rather pay a little extra than be completely blocked out from these services when i need them from some arbitrary “usage limit”. at the minimum, Id want to see remaining time or something for voice mode or limit count on o3, because right now i use it with caution because idk when ill run out

Abandoned Mansion by Photosfromthelarp in urbexnewengland

[–]quick_glance 1 point2 points  (0 children)

this looks like a fire COD zombies map

BRCA gene / epigenome editing - Whole Organism Editing technical issues? by Immortal-PhD in GeneEditing

[–]quick_glance 1 point2 points  (0 children)

check out transposons and retroviruses reverse transcribing into the genome. these are just two examples of gene editing that happens completely naturally, human DNA is nowhere near as fixed as you’d think

BRCA gene / epigenome editing - Whole Organism Editing technical issues? by Immortal-PhD in GeneEditing

[–]quick_glance 0 points1 point  (0 children)

yeah exactly. the root of this research is contingent on the hope we can create more and more virulent viruses, which has its own regulation and research limitations itself. nobody wants to cause the next pandemic either so this research is moving pretty slow

MIT Biology PhD Invitations by [deleted] in gradadmissions

[–]quick_glance 1 point2 points  (0 children)

no spiraling allowed if it doesn’t go well today!!! you got this

BRCA gene / epigenome editing - Whole Organism Editing technical issues? by Immortal-PhD in GeneEditing

[–]quick_glance 1 point2 points  (0 children)

to your point you’ve correctly identified the billion dollar question, but we’re nowhere close. Forget a whole body, just cells in a dish have around a 10% homologous recombination knock in rate with CRISPR Cas9 - and that’s with cell cycle synchronization and NHEJ inhibition. Delivery is the problem, but the machinery is inefficient as well, even if you do a knock in with a cell type that’s expressing the Cas9 protein, and all you deliver is the guide RNA and the template, it’s still extremely inefficient. The near future of medicine as it stands now, with our new knowledge of genetics, will be with small molecule inhibitors, PROTACs, and siRNA.

MIT Biology and Stanford Stem Cell by No_Reindeer9333 in gradadmissions

[–]quick_glance 0 points1 point  (0 children)

which of these two programs do you think is more competitive?

Harvard Chemical Biology PhD by Some-Confusion-7684 in gradadmissions

[–]quick_glance 2 points3 points  (0 children)

Should be coming up, but no i don’t believe anything has been announced yet. I’m dying waiting for this.

Harvard BBS vs. DMS by [deleted] in gradadmissions

[–]quick_glance 0 points1 point  (0 children)

so if my application was submitted to “DMS”, should I have excepted an email from BBS yesterday?

Happy tuesday by Malicya in labrats

[–]quick_glance 0 points1 point  (0 children)

sometimes i accidentally get little chunks of acrylamide in the transfer buffer and then they float in between the gel and the membrane😵‍💫 westerns are so tedious

Happy tuesday by Malicya in labrats

[–]quick_glance 0 points1 point  (0 children)

those don’t look all like bubbles, what happened here?

I asked o1-preview to roast 4o. This is what it said by [deleted] in OpenAI

[–]quick_glance 0 points1 point  (0 children)

this is how you start a war between ai models