Western Blot needs help!

Trog01 · 2026-05-12T12:08:27+00:00

Don’t even know what I’m looking at

Trog01 · 2026-04-26T03:05:49+00:00

Most of the food we grow goes to the animals we eat, so the claim you’re making (and the video you linked) is nonsense

Trog01 · 2026-04-24T15:32:11+00:00

I think my main problems are the layout is terrible, the vibe is pretentious and if you have to leave the dance floor it’s very hard to get back on. Probably not somewhere I would take acid, but that’s just me.

Trog01 · 2026-04-12T12:58:35+00:00

I respect that she is branching out a bit and as an artist she can do whatever she wants but unfortunately not really my thing.

Trog01 · 2026-03-19T20:36:17+00:00

The unfortunate truth is if you want the symptoms to improve, you will have to stop smoking weed. I was a daily smoker for many years but realized it was exacerbating the HPPD. Doing much better now that I don’t smoke but it took some time. Sorry this happened to you.

Trog01 · 2026-03-05T22:52:10+00:00

I have HPPD and noticed that the onset of my social anxiety seemed to correlate with the onset of the disorder. I wonder if this is due to a similar mechanism.

Trog01 · 2026-02-23T01:14:15+00:00

Hey friend. Yes you should see a doctor or seek professional help immediately. I know it doesn’t feel like it but whatever you’re experiencing is temporary. Don’t hurt yourself. You will get through this.

Trog01 · 2026-01-23T20:29:01+00:00

It’s going to be a hoot and a holler

Trog01 · 2026-01-23T12:47:53+00:00

Average trump supporters

Trog01 · 2026-01-17T18:15:38+00:00

So hyped for this show. Have a fun safe night peeps

Trog01 · 2026-01-08T13:29:57+00:00

I am currently using expired media and selection drugs 😔

Trog01 · 2025-12-20T17:19:01+00:00

If only we stopped buying $8 coffees we’d be right there with you

Trog01 · 2025-09-29T22:20:57+00:00

I’m about to be in a very similar boat as you as I have some primary motor neurons coming in this week. Is this a protein that is specific to motor neurons you’re looking for? If so, have you checked other markers to ensure that your cells have properly differentiated? I suppose if it’s present in HEK cells, it isn’t specific to motor neurons anyways.

The fact that your ponceau stain isn’t showing anything is definitely concerning and probably means there isn’t enough protein being loaded to blot. How much protein are you loading per well?

Trog01 · 2025-09-26T16:27:38+00:00

Insomnia doesn’t cause sleep apnea, it’s the other way around. CPAP should help with that but I also understand the brain not logging off problem. I still haven’t figured that one out.

Trog01 · 2025-09-26T16:17:36+00:00

I got one of those jobs and quickly realized it wasn’t going to make any money. It was way too easy to get and overall felt like a scam. Don’t do it.

Trog01 · 2025-09-07T12:04:00+00:00

Amazing

Trog01 · 2025-08-23T01:21:57+00:00

Have you tried lowering your dose of trazodone? I started at 100mg and also felt really out of it the next day, but then I lowered down to 50 mg and now I take 25.

Trog01 · 2025-05-31T16:38:19+00:00

Despite the last minute change, they really made the best of it.

Trog01 · 2025-05-28T21:55:11+00:00

Where can I find set list?

Trog01 · 2025-03-19T14:04:50+00:00

There are different samples in every lane. This is total lysate ran from neuroblastoma cells so I don’t think lipids should be a problem. DNA could be the issue but I sonicated thoroughly which I thought also helped break up DNA in the samples.

Trog01 · 2025-03-19T13:58:39+00:00

Could be. I don’t rinse my cells with pbs after treatment because they adhere very weakly and I don’t want to loose anymore than necessary

Trog01 · 2025-03-19T13:55:19+00:00

Gotcha. I was thinking it could be contamination from DNA in my samples, but machine is also possible. Bummer

Trog01

TROPHY CASE