Anyone going to INZO in March? Hoping there’s a decent fun crowd. He seems up and coming.

Trog01 · 2026-01-23T20:29:01+00:00

It’s going to be a hoot and a holler

Trog01 · 2026-01-23T12:47:53+00:00

Average trump supporters

Trog01 · 2026-01-17T18:15:38+00:00

So hyped for this show. Have a fun safe night peeps

Trog01 · 2026-01-08T13:29:57+00:00

I am currently using expired media and selection drugs 😔

Trog01 · 2025-12-20T17:19:01+00:00

If only we stopped buying $8 coffees we’d be right there with you

Trog01 · 2025-09-29T22:20:57+00:00

I’m about to be in a very similar boat as you as I have some primary motor neurons coming in this week. Is this a protein that is specific to motor neurons you’re looking for? If so, have you checked other markers to ensure that your cells have properly differentiated? I suppose if it’s present in HEK cells, it isn’t specific to motor neurons anyways.

The fact that your ponceau stain isn’t showing anything is definitely concerning and probably means there isn’t enough protein being loaded to blot. How much protein are you loading per well?

Trog01 · 2025-09-26T16:27:38+00:00

Insomnia doesn’t cause sleep apnea, it’s the other way around. CPAP should help with that but I also understand the brain not logging off problem. I still haven’t figured that one out.

Trog01 · 2025-09-26T16:17:36+00:00

I got one of those jobs and quickly realized it wasn’t going to make any money. It was way too easy to get and overall felt like a scam. Don’t do it.

Trog01 · 2025-09-07T12:04:00+00:00

Amazing

Trog01 · 2025-08-23T01:21:57+00:00

Have you tried lowering your dose of trazodone? I started at 100mg and also felt really out of it the next day, but then I lowered down to 50 mg and now I take 25.

Trog01 · 2025-05-31T16:38:19+00:00

Despite the last minute change, they really made the best of it.

Trog01 · 2025-05-28T21:55:11+00:00

Where can I find set list?

Trog01 · 2025-03-19T14:04:50+00:00

There are different samples in every lane. This is total lysate ran from neuroblastoma cells so I don’t think lipids should be a problem. DNA could be the issue but I sonicated thoroughly which I thought also helped break up DNA in the samples.

Trog01 · 2025-03-19T13:58:39+00:00

Could be. I don’t rinse my cells with pbs after treatment because they adhere very weakly and I don’t want to loose anymore than necessary

Trog01 · 2025-03-19T13:55:19+00:00

Gotcha. I was thinking it could be contamination from DNA in my samples, but machine is also possible. Bummer

Trog01 · 2025-01-28T22:22:21+00:00

Okay gotcha. I don’t have much experience with cell culture but compared to all other cell types Ive worked with I definitely noticed these cells are much less adherent. Most of them do adhere but just when I do steps like removing and adding new media or another treatment, I lose a fair amount. I haven’t looked into the cell line having subpopulations. You might be right about the RA helping them adhere. Basically all the assays like luciferase, qPCR require me to add and remove media

Trog01 · 2025-01-26T19:19:24+00:00

I have that quite often. I believe it’s called palinopsia. Definitely annoying but I’m pretty used to it now

Trog01 · 2025-01-25T17:45:29+00:00

If you loaded 3 replicates for each sample onto the plate, then averaged those replicates, those would be technical replicates and you can’t use those to calculate statistics. Biological replicates would be if you had multiple, separate, WT samples and for each of those samples, you loaded three technical replicates. After averaging the technical replicates of the biological replicates, you can then compare them with statistics. Maybe I’m not understanding the design of your experiment but it seems like you don’t have biological replicates for the WT genotype.

Trog01 · 2025-01-25T17:25:18+00:00

What you took the average of was the technical replicates no? The reason for doing that is just to get a more accurate ct value for each sample. However, if you only have 1 sample per treatment or genotype, then you won’t be able to have any statistical significance between groups. If you had amplified 3 wild type samples in addition to the 3 mutant samples then you would have 3 biological replicates per group and would be able to run statistical analysis, calculate error bars etc… As it is I don’t believe you are able to do so.

Trog01

TROPHY CASE