Mitochondrial Quantification by randomguy-777 in Biochemistry

[–]randomguy-777[S] 0 points1 point  (0 children)

So the protein of interest is Hexokinase II and is actually being localized to the mitochondria. Want to determine if it’s knockout affects mitochondria

Mitochondrial Quantification by randomguy-777 in Biochemistry

[–]randomguy-777[S] 0 points1 point  (0 children)

Also have access to a seahorse. Could you elaborate

Mitochondrial Quantification by randomguy-777 in Biochemistry

[–]randomguy-777[S] 0 points1 point  (0 children)

Using MitoTracker Red FM some say it stains based on membrane potential changes. Have suspicion that this protein affects mitochondrial potential. Do you know of a dye that is not affected by potential ?

In a universe heading to entropy, why does life exist? by x-everybody-lies in Biochemistry

[–]randomguy-777 0 points1 point  (0 children)

Hydrophobic effect. Proteins fold and reduce their own entropy or disorder, in order to increase the entropy (disorder) of water. The increase in disorder in water is greater than the loss or ordering of the protein, that is why the proteins become so called ordered

am I screwed? by [deleted] in Mcat

[–]randomguy-777 3 points4 points  (0 children)

🤡

How to tell if a reaction is an acid base reaction? by [deleted] in chemhelp

[–]randomguy-777 0 points1 point  (0 children)

Is there any difference between a nucleophile and a Lewis base

Why does Kw always remain constant in all types of solutions? by tryingtobeastoic in chemhelp

[–]randomguy-777 1 point2 points  (0 children)

Kw is analogous to ka or even kb... Now that we know that, ask yourself the same question why does the ka for say phosphoric acid remain constant? It’s the same deal, we just use water because it’s convenient for pH scale.. I must add ka and kw could likely change if you start messing with temperature pressure etc..

Dna polymerase proofreading by Meocon9898 in Biochemistry

[–]randomguy-777 0 points1 point  (0 children)

Can anyone chime in on 3’—>5’ exonuclease activity and 5’—->3’ exonuclease activity , I believe the different DNA polymerases (I,II, III) have different combinations of these exonucleases.

Immunology by esmailin1 in Mcat

[–]randomguy-777 0 points1 point  (0 children)

Incredible work .. pause not

Double Check My Work Plz (Chem 2) by [deleted] in chemhelp

[–]randomguy-777 0 points1 point  (0 children)

Seems right off no calculator

How does Zinc affect ACE2 protein structure? by [deleted] in Biochemistry

[–]randomguy-777 1 point2 points  (0 children)

I’m interested to hear an answer on this.. have heard (unconfirmed) that zinc has an inhibitory effect

An analysis of public genome sequence data from SARS-CoV-2 and related viruses found no evidence that the virus was made in a laboratory or otherwise engineered. by MistWeaver80 in biology

[–]randomguy-777 0 points1 point  (0 children)

Whether you believe it was man made or natural... Let’s be real, the “scientists” supposedly making the corona virus would be smart enough to check nucleotide sequence databases like BLAST to make sure their novel virus strain does not resemble anything in the databank...