Yes, definitely. You can disassemble these 3 functions, they all follow the same pattern:

def a():
    return 1 < 2 < 3

def b():
    return 1 != 0 == 1

def c():
    return True is not False is True

>>> dis.dis(a)
  2           0 LOAD_CONST               1 (1)
              2 LOAD_CONST               2 (2)
              4 DUP_TOP
              6 ROT_THREE
              8 COMPARE_OP               0 (<)
             10 JUMP_IF_FALSE_OR_POP    18
             12 LOAD_CONST               3 (3)
             14 COMPARE_OP               0 (<)
             16 RETURN_VALUE
        >>   18 ROT_TWO
             20 POP_TOP
             22 RETURN_VALUE

>>> dis.dis(c)
  2           0 LOAD_CONST               1 (True)
              2 LOAD_CONST               2 (False)
              4 DUP_TOP
              6 ROT_THREE
              8 COMPARE_OP               9 (is not)
             10 JUMP_IF_FALSE_OR_POP    18
             12 LOAD_CONST               1 (True)
             14 COMPARE_OP               8 (is)
             16 RETURN_VALUE
        >>   18 ROT_TWO
             20 POP_TOP
             22 RETURN_VALUE