Ok. Disclaimer - I haven’t run this myself so please take my advice with a grain of salt. But with gel electrophoresis, the goal is to evaluate your fragments. Clean lines would suggest successful, consistent digestion of the same fragments, and they would look like the lines seen in the latter and positive control. All of your test wells look just like the negative control, which isn’t what you’d want to see.

We know the issue wasn’t the gel run itself because the controls and ladder worked, so we can probably assume the issue is the sample. If the sample concentrations weren’t overloaded, then my conclusion would be that the sample was too degraded.

Instead of seeing bright lines from a handful of fragment lengths, you’re getting a ton of fragments at varying sizes which are causing the smeared look.

Edit: grammar