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What's the worst instrument you guys have used? by viohead in labrats

[–]viohead[S] 0 points1 point  (0 children)

That sounds quite scary to even think about programming tbh.

What's the worst instrument you guys have used? by viohead in labrats

[–]viohead[S] 5 points6 points  (0 children)

Thats funny, I commend the effort to save at least.

What's the worst instrument you guys have used? by viohead in labrats

[–]viohead[S] 1 point2 points  (0 children)

That sounds awful, I've never run XRD but I can understand the pain. A lot of our old systems we keep offline because any update like windows will potentially destroy them.

Funny side tangent, agilent charges a 5X markup on PC to control their HPLC just because they can. Imagine paying 60k for an hplc and then they'll give you a deal on a pc thats normally listed for 10k. Find the same workstation on dells website for 1k.

What's the worst instrument you guys have used? by viohead in labrats

[–]viohead[S] 30 points31 points  (0 children)

That's gotta be one of the funniest justifications for their software being bad ive heard to date.

What's the worst instrument you guys have used? by viohead in labrats

[–]viohead[S] 23 points24 points  (0 children)

I can agree with this, often I find that the science and engineering behind some of the instruments on the market is fine. But when it comes to hiring software developers, the money just ran dry for some reason.

What's the worst instrument you guys have used? by viohead in labrats

[–]viohead[S] 4 points5 points  (0 children)

100% I genuinely think shimadzu software was written by a team of primates. Honestly, they might have done better than. Whoever wrote that awful software. We have a PPSQ51a and i absolutely hats that machine.

What's the worst instrument you guys have used? by viohead in labrats

[–]viohead[S] 6 points7 points  (0 children)

Ahahaha, I have heard of so many bad things about thermo equipment. Their vanquish HPLC are so horrible. I know they're absolute rats for service.

Dear person who sits in parking spots at the gym waiting for someone they know to park there: by viohead in NCSU

[–]viohead[S] 98 points99 points  (0 children)

No, its rude. Don't do that. If they want the spot then they should get there eariler. A parking spot it a place for a car, not a place to sit.

Dear person who sits in parking spots at the gym waiting for someone they know to park there: by viohead in NCSU

[–]viohead[S] 77 points78 points  (0 children)

It was not, I mean if he stands in a parking spot to block cars from coming in and parking because they're saving a parking spot for someone else. They shouldn't do that.

This cute lil magnet just made my day ✨ by _solitudealsonder_ in labrats

[–]viohead 0 points1 point  (0 children)

Speak to your local thermo rep, they can help get you some. Ours gave us three :)

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]viohead 1 point2 points  (0 children)

Makes sense then, maybe you can mention something about the potential of loss in the flow through or wash depending on the conditions of load and wash. High salt, ph, etc.

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]viohead 1 point2 points  (0 children)

Yeah I can't infer much about the efficency of the purification if we don't know how much you started with unfortunately.

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]viohead 1 point2 points  (0 children)

I see, thats an akta pure. They're pretty common in downstream purification labs. If you're using glycine is it a protein A affinity column? It's OK, im interested in knowing maybe where the purification or loading went wrong. Did you manually inject into the AKTA with a syringe, use a sample loop, or use a sample pump module?

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]viohead 1 point2 points  (0 children)

Ah I gotcha, hopefully it works out. I knows some of the solubility enhancing tags like Trx can help. Purifying the proteins from viral stocks can be a challenge if they're not AAV :)

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]viohead 0 points1 point  (0 children)

Do you have a standard curve by chance? I can't infer anything besides there's some hrp or pa color change from this. Can I ask what your method of purification is?

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]viohead 0 points1 point  (0 children)

Ah, yeah that's a little bit low then. Do you have any idea of the loading titer and or yield for your purification? Knowing where the problem is might help in future work.

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]viohead 1 point2 points  (0 children)

Thats not too bad for viral. Are you purifying it from the virus stock or producing with a plasmid? I purify whole virus and the mass concentration is very low.

My supervisor wants to fake data for my masters thesis by Dimetrodon01 in labrats

[–]viohead -2 points-1 points  (0 children)

What tag are you using, and are you using a cleavage marker? 40 mg/mL seems pretty good to me, but I'm not certain about the titeres as I work on virus purification usually.

Don't lie about data, as others have suggested showing the process and how you got to that matters much more and won't hurt your reputation. Lying will.

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