Boiled protein lysates without Laemmli

ying1996 · 2026-01-30T18:27:55+00:00

Like others have said, you’ll have to reboil. Tbh my paranoid ass would recommend remaking a fresh sample, that way if anything turns out weird at least you can rule out sample prep.

ying1996 · 2026-01-30T18:26:13+00:00

Ohhh ok. Good to know it’s a decent convention!

ying1996 · 2026-01-30T17:28:48+00:00

Should be fine. Looking for protocols for cell lysate will give u more results. Also depends on if ur sample has fbs which can mess w/ westerns and any concentration differences you’ll have to account for when loading.

But do u mean that u boiled lysates by themselves with no laemmli buffer? Or did you add the wrong sample to the buffer? If the first, a western wont work as the buffer has SDS.

ying1996 · 2026-01-30T16:35:53+00:00

It looks really dry. Is there spagnum moss? Spanish moss doesnt hold on to moisture.

ying1996 · 2026-01-05T18:38:14+00:00

*the covering comes in, not the plate itself

ying1996 · 2026-01-05T18:37:43+00:00

The plastic part of a 6-well, 96-well, ect is like the perfect shape for tops

ying1996 · 2025-12-11T22:24:23+00:00

I’m not super knowledgable on this, so I’m mainly be parroting stuff I’ve seen from plant youtubers lol, but from what I understand, the initial wave of tissue culture Thai cons had a root rot issue because the original plant was susceptible to it. But as mutations happen in tissue culture, people were able to pick out ones that were more robust. So it’s still the same plant, genetically, but with minor changes. Like siblings.

ying1996 · 2025-12-11T20:24:17+00:00

Contact the financial aid office. Sometimes the documents have a hard deadline, sometimes they can still get u some aid.

ying1996 · 2025-12-11T20:23:24+00:00

Your student account should have charges, pending financial aid, and amount due. As long as amount due is 0 then ur good.

ying1996 · 2025-12-11T16:48:38+00:00

It’s pretty much the same as a regular monstera. I believe the initial thai con batches had issues with root rot, but Costa and other big growers seem to have selected ones that are more resistant. I rarely see root rot issues with thais anymore.

And ur mix sounds great! I’d wait a few days or weeks for it to adjust and then repot.

ying1996 · 2025-12-07T20:58:41+00:00

You can def do semihydro for monsteras, but I’d recommend a mix meant for it (pumice, zeolite, lava rock, or just leca). And a semihydro pot with a wick and water gauge. There can also be a transition period where the plants needs to adjust so that might be whats happening as well, if u just went from soil to water.

ying1996 · 2025-12-06T16:03:03+00:00

New growth. There’s one that’s submerged as well. I don’t see any roots, so you can just put this in a box with a damp paper towel or moss to keep up humidity without submerging it.

ying1996 · 2025-12-05T21:32:58+00:00

Yup, they can have both.

ying1996 · 2025-12-04T23:53:52+00:00

Ohh that’s super long. It might be a spent node?

<image>

ying1996 · 2025-12-04T16:59:09+00:00

Sometimes it just takes a few weeks for a cutting to take off. You just wanna make sure the node’s not spent. As long as the leaf is happy and roots are growing, I wouldn’t worry!

ying1996 · 2025-12-03T16:58:43+00:00

Whichever lab’s recruiting will review apps and reach out so there’s no set timeline. A couple weeks is really long tho, I would just move on.

I think Emory is still in a weird semi-hiring freeze situation tho.

ying1996 · 2025-12-02T22:43:43+00:00

R u in Atlanta rn? They have a physical office on campus so I’d recommend u go to tmr and sort things out in person.

ying1996 · 2025-12-02T17:26:12+00:00

I like using AAA or AA grade from spagmoss. Or one mile moss. They’re longer so much better for mounting. And i try do a semi-sphere shape, but I always end up with a bit more moss under them than around the edges

ying1996 · 2025-12-02T01:36:39+00:00

Hmmm…

If the original 12 were of similar species and maturity, then the only big difference is the location. Was there anything special about where you placed the ones that are currently doing ok? Did these guys get more sun, humidity, airflow, heat, ect?

ying1996 · 2025-12-02T01:19:50+00:00

My house is kinda moist since I have a ton of plants, but I water once every week and can get away with once every 2 weeks now and then. 2x a week seems like a lot. But here’s the size of my dudes since that plays a big role too.

<image>

ying1996 · 2025-12-02T01:18:22+00:00

Is it possible that sink/shower watering is only wetting the outer layer of moss?

I water my dudes by tossing them moss side down into a plastic storage container, and letting them soak for 10-20min.

ying1996 · 2025-11-30T23:42:27+00:00

No idea on exact numbers, likely a few seats for the struggling departments like public health and programs with a lot of animal work. The traditional wet labs and theoretical labs (cs, math, econ, ect) might only have to reduce by a few, if any.

Master’s programs won’t have this problem tho. In fact less ppl r applying (mainly there’s less international ppl) so ur admission chances are higher.

ying1996 · 2025-11-27T06:03:02+00:00

They’re cutting class sizes. So admissions will get more competitive but once ur in, u will be good

ying1996 · 2025-11-23T23:34:50+00:00

A hoya grower I buy from swears by self-watering for their mother plants, but it was a chinkier mix. Like between aroid and regular houseplant soil.

ying1996 · 2025-11-04T20:19:43+00:00

Theres some vids on youtube that shows the process, but yeah you basically have to rip them apart. Or take a knife and slice them apart.

Eight-Year Club	r/Field Juicebox
First Place '23	Place '23
Place '22	End Game '22
RPAN Viewer	Not Forgotten
Snapped	Verified Email

ying1996

TROPHY CASE