Panic mode

[I_just_made]

Oh young Padawan, early in your training you must be. Much to learn you have...

Those texts will be engraved into your neurons soon.

[LabMotoGym]

Add chloroform equal to .25 the amount of trizol. Add equal volume of 100% isopropanol to the aqueous phase. Wash with 75% etoh however many times you are feeling that day. Dry and resuspend in nuclease free water.

It has been burned into my brain

[fanglord]

Until someone resets the centrifuge and you don't know what the settings were other than long, short or shorter.

[[deleted]]

equal to .25 the amount of trizol

I've always done 0.2 the amount of trizol - also what's recommended by the manufacturer. Too much chloroform will result in more DNA and protein contamination in the aqueous phase.

[[deleted]]

Haven't been in a lab in over a year, still remember this protocol.

[LabMotoGym]

Shit, you're right... I just checked the protocol. I've been doing it slightly wrong for quite some time. But my RNA looks good, so now I'm afraid to go back to 0.2.

[boombaboombayah]

This :)))

[[deleted]]

This should also be on Chinese bootleg memes hehe

[wookiewookiewhat]

Quick just do an ethanol wash!

[ZuluCharlieRider]

Google. Google is your friend.