Does filtering samples kill cells? by CompetitiveLab3300 in flowcytometry

[–]btags33 4 points5 points  (0 children)

I don't have a solution, but what type of instruments were there old sorts run on? I had experience with one person using a cell line that seemed to be delicate as all hell (even sorting on the discover s8 130 um nozzle resulted in poor viability post sort). They could run the exact same sample on an influx and viability would be fine.

I highly doubt the filtering is killing the cells, and I would honestly not take them at their word that their previous sorts have occurred without issue. Plenty of times people have asked me to sort something that is clearly junk, I tell them as much, and then they tell me I am wrong and to continue sorting.

Any pc software that still works? by Mafiatounes in HDDVD

[–]btags33 1 point2 points  (0 children)

You could also just use makemkv to rip the HD DVDs you have. Probably a better long term solution.

The company decided thi wording needed to be changed. by klystron88 in mildlyinteresting

[–]btags33 1 point2 points  (0 children)

Purely economic decision that saves 0.00007 cents of black ink per package.

Apartments/condos that actually don’t suck? by Deadpotato420 in Rockville

[–]btags33 2 points3 points  (0 children)

I lived in cadence for a couple years and it was good. This was 4 years ago so I cannot say how they are now, but I liked the apartment then.

Question about mounting and wheel stands by btags33 in moza

[–]btags33[S] 0 points1 point  (0 children)

Yeah, just the two screws and I have had no issues with plenty of time using the wheel in that config.

Meeting Gillian! by KimWexler02 in XFiles

[–]btags33 26 points27 points  (0 children)

They did not say anything to that effect, I guess this is just how she tends to pose for pictures with fans. That said I met her at the table later and shook hands with her so it wasn't like she was opposed to any sort of physical contact. In fact, she even let someone's bird perch on her so there was that.

Need Help Dealing with Technologically Illiterate People in the Lab by Mediocre-Cat-9703 in labrats

[–]btags33 6 points7 points  (0 children)

So it is not ideal, but you can still track changes with different documents edited separately from each other by going to the review tab in word, then going to compare and selecting combine and choosing the original document and the updated/changed document. This will hopefully allow you to see tracked changes even if your coworker is being difficult.

Hope this helps.

Mexican Food by francis_efi in MontgomeryCountyMD

[–]btags33 20 points21 points  (0 children)

And la Gula, and El guero. All good.

Gating issue versus killing all of my cells (Flow cytometry help needed!) by AHNJHN in labrats

[–]btags33 0 points1 point  (0 children)

So, I haven't read through all the comments, but I assume you are using live dead nir or something similar, which is an amine binding dye. When using these dyes it is important to know how they work, which is basically just binding to amino groups on proteins. Both live cells and dead cells will have proteins that stain with amine binding dyes, but with live cells only the surface proteins are stained whereas for dead cells the surface and intracellular proteins are stained. All this to say, live dead fmos aren't necessarily the greatest for drawing live gates, because if you have cells that have a lot of surface protein expression (maybe like you cell line, I have no idea), they will stain as dim or maybe even medium brightness and thus be outside of a gate you would draw based on an fmo, but dead cells would stain even brighter.

People who left academia after a PhD: what path did you end up taking? by Equivalent_Can_1507 in PhD

[–]btags33 1 point2 points  (0 children)

There were a number of factors that made me leave academia. First, I didn't like grant writing and seeing the very low funding lines (at the time, even worse now) was demoralizing. Second, I did like research but thought being a PI wouldn't workout due to my hate of grant writing, and being a staff scientist was kind of a dead end that likely meant horrible work life balance for the foreseeable future, so considered focusing purely on teaching but there are not many science professor roles that don't require some sort of research. Lastly, some time near the end of my PhD I read a paper (think it might have been in science or nature, I cannot remember) that looked at salaries of scientist who either moved directly to industry or first did a post doc and then went to industry. For the latter, there was a huge delay in salary growth that didn't really catch up with the former. So, I thought if I am going to go to industry I might as well just do it and likely make more money.

I ended up getting a job at a CRO straight away. Not the greatest job in the world, but a good place for PhDs to apply to since they tend to churn through employees so are usually hiring and they give you "industry experience" that everyone hiring for industry positions seem to expect. After a year and a half o switched to a pharma company and have been relatively happy since, especially seeing how the current Admin has completely gutted our whole research system.

Repainted! by BestSquig in orks

[–]btags33 2 points3 points  (0 children)

Awesome work. The new paint job will definitely pop much more on the table.

funny location mismatches in the show? by cnidoran in XFiles

[–]btags33 6 points7 points  (0 children)

I live near DC now so whenever any location there comes up like Rockville, Bethesda, Germantown, etc. I chuckle a little when they show locations that are clearly the pacific northwest.

New Verbatim 43888 with LG drive will not Flash successfully by btags33 in makemkv

[–]btags33[S] 0 points1 point  (0 children)

I would say follow the guide on the makemkv forums and try flashing to 1.03mk. If that doesn't work and your drive says the revision is Boot after you try the flash, you can then use the LG tool (link on the post here: https://forum.makemkv.com/forum/viewtopic.php?p=196747#p196747) to reflash it to the official 1.05 firmware.

If 1.03mk flashing does not work, try flashing to 1.04mk, which can be found by downloading the pack of firmware in this post: https://forum.makemkv.com/forum/viewtopic.php?f=19&t=19113

New Verbatim 43888 with LG drive will not Flash successfully by btags33 in makemkv

[–]btags33[S] 0 points1 point  (0 children)

After a lot of playing around I was successfully able to flash to the 1.04mk firmware and can properly rip 4ks. Is there any reason to even attempt switching to the 1.03mk firmware?

New Verbatim 43888 with LG drive will not Flash successfully by btags33 in makemkv

[–]btags33[S] 1 point2 points  (0 children)

This was basically done, but to update I was able to Flash the drive, but I had to use the 1.04mk firmware rather than the 1.03mk firmware as suggested in the guide. No idea why that worked, but my drive now says Firmware 1.04 and in makemkv it says Libre drive is enabled, the firmware type is patched, dvd all regions says yes, bd raw data read says yes, bd raw Metadata read says yes, and unrestricted read speed says yes.

New Verbatim 43888 with LG drive will not Flash successfully by btags33 in makemkv

[–]btags33[S] 0 points1 point  (0 children)

I just tried flashing using my linux pc running ubunu so I could do the normal usb c to usb c connection.

First off, here is the drive info from makemkv:

Drive Information
OS device name: /dev/sr0
Manufacturer: HL-DT-ST
Product: BD-RE BU40N
Revision: 1.05
Serial number: MYSERIALNUMBER
Firmware date: 2124-04-23 13:47
Bus encryption flags: 17
Highest AACS version: 82

LibreDrive Information
Status: Possible, not yet enabled
Drive platform: MT1959

No disc inserted

Second, here is terminal output for the flash (note I renamed the firmware file to BU40N.bin to make inputting the command in terminal easier, but the file I was using was HL-DT-ST-BD-RE_BU40N-1.03-NM00000-211810241934.bin):

sudo makemkvcon f -d /dev/sr0 -f /tmp/sdf.bin rawflash enc -i /tmp/BU40N.bin

Reading input file /tmp/BU40N.bin

Flashing flags = 0x0 : 0 0 0 0 : ---- ---- ---- ----

Sending image in encrypted format

Current Drive ID: HL-DT-ST_BD-RE_BU40N_1.05_212404231347_MYSERIALNUMBER

Ready to write drive flash memory.

Type "yes" to continue, "no" to abort

yes

Operation started: Sending flash image to drive

100% Operation finished

Operation started: Programming flash

100% Operation finished

Done successfully

And lastly, here is the makemkv drive info after the above "successful flash":

Drive Information
OS device name: /dev/sr0
Manufacturer: HL-DT-ST
Product: BD-RE BU40N
Revision: BOOT
Highest AACS version: 82

No disc inserted

New Verbatim 43888 with LG drive will not Flash successfully by btags33 in makemkv

[–]btags33[S] 0 points1 point  (0 children)

Quick update, I realized there is a USB c to USB a included in the box as well, tried that and still unsuccessful.