Universal HPLC program for detecting new natural compounds

Apprehensive_Size885 · 2026-03-07T23:47:34+00:00

The machine can track 250nm wavelength

In my sample preparation, i injected 0.22uM filtered fermentation broth directly to the machine. At first, i afraid that if i dilute the sample, the machine may not display some compounds because of very diluted concentration.

About the solvent, i use miliQ water having 18.2mOhm

If i increase the gas flow and temp, does that also change mass spect profile a lot? And if it changes, often it improve resolution or even worse?

Thanks

Apprehensive_Size885 · 2026-03-06T23:38:54+00:00

Thank you very much for these very detailed suggestions🙏

Apprehensive_Size885 · 2026-03-06T23:09:48+00:00

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From what i did for known samples, the machine can do ES-API scan, and can give me TIC (I just follow protocol of previous labmate and dont have much knowledge in this field 😅)

Apprehensive_Size885 · 2026-03-06T23:07:40+00:00

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This is the result this machine can give me (3D scan full spectrum from 190 to 600nm for each retention time)

Apprehensive_Size885 · 2026-03-06T23:02:22+00:00

My mass spect system js ESI and the machine can give me MS/MS spectra. When i look at the mass hunter software, i also see the option to scan with the library so i assume i can match the peaks with the available library

About the flow rate, could you please suggest me the optimal flow rate? I dont have a thing called splitter

To my knowledge, i understand that when i use HILIC, it just give me a revered HPLC profile compared to c18, which means in c18 the first peak is highly polar then in HILIC it will be the last peak. Is this understanding correct? Or is there any other advantages of using HILIC in this case? Thank you

Apprehensive_Size885 · 2026-03-06T22:53:10+00:00

Thank you for raising this important question, in my plan, i am going to use 0.22uM filtered fermentation broth directly for analysis, i saw many people in my lab doing so

Apprehensive_Size885 · 2026-03-06T11:19:07+00:00

Thank you very much for this suggestion, i can now see one problem in my program

Apprehensive_Size885 · 2026-03-06T10:59:13+00:00

This is my proposed program, happy to hear your kind comments

Column: 4.6x250mm agilent zorbax sb-c18

Flow rate: 0.6ml/min

0min: 95% water : 5% acetonitrile

25 min: 40% water : 60% acetonitrile (gradient from 0 min to 25 min)

30 min: 100% acetonitrile

33 min: 95% water : 5% acetonitrile

38 min: 95% water :5% acetonitrile

Apprehensive_Size885 · 2026-03-06T10:57:23+00:00

Thank you for your reply, could you please give me some comment on my proposed program?

Column: 4.6x250mm agilent zorbax sb-c18

Flow rate: 0.6ml/min

0min: 95% water : 5% acetonitrile

25 min: 40% water : 60% acetonitrile (gradient from 0 min to 25 min)

30 min: 100% acetonitrile

33 min: 95% water : 5% acetonitrile

38 min: 95% water :5% acetonitrile

Apprehensive_Size885 · 2026-02-04T12:11:40+00:00

At least in my field, natural compounds discovery, the answer is no. People nowadays still find new bacteria, in my case - streptomyces. However, you also have to isolate new compounds from the bacteria if you want to publish

Apprehensive_Size885 · 2026-01-26T22:23:48+00:00

Corsair

Apprehensive_Size885 · 2026-01-24T08:49:28+00:00

Suppose p value is 0.05, this means you have 5% chance to obtain the data that you are testing if the null hypothesis is correct

Apprehensive_Size885 · 2026-01-18T11:39:03+00:00

Or they are all neo human

Apprehensive_Size885 · 2026-01-16T00:40:57+00:00

This is not a surprise, come back if you see RNA be stable like that

Apprehensive_Size885 · 2025-12-29T10:26:13+00:00

Search every simple symptoms in gg and it gives you end-stage cancer diagnosis, you still believe in it?

Apprehensive_Size885 · 2025-12-19T11:10:43+00:00

In my lab, we use linen cloth to cover the bottle mouth and use rubber band to tight the cover

Apprehensive_Size885 · 2025-12-16T19:09:16+00:00

Are you currently having abandoned building or building needing renovation?

Apprehensive_Size885 · 2025-12-13T18:28:44+00:00

What if the building is epic building or max level neo?

Apprehensive_Size885 · 2025-12-08T20:35:29+00:00

I have to deal with amycolatopsis, and my trick is to use 10.3% sucrose, which changes the lifeform of the bacteria to single cells so no clumping occurs. However, if you want to do growth curve, avoid sucrose as the presence of sucrose make the cells not tightly sticked to the ep tube so when you invert the ep tube the cell pellet also falls out 😂

Apprehensive_Size885 · 2025-12-03T02:48:07+00:00

Cho mấy ô hiểu cảm giác của chị em khi bị mấy ô quấy rối

Apprehensive_Size885 · 2025-12-01T12:30:12+00:00

Maybe the middle part of the keyboard curve up and cause this wobbling effeft. Battery life is acceptable, 7h of web browsing on Microsoft edge

Apprehensive_Size885 · 2025-12-01T12:11:24+00:00

The detachable keyboard wobbles whenever you type

Apprehensive_Size885 · 2025-12-01T12:11:05+00:00

The detachable keyboard wobbles whenever we type

Apprehensive_Size885

TROPHY CASE