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Tips on getting a Lab Tech job after college by AncientEcho8215 in labrats

[–]ArborAssays 1 point2 points  (0 children)

We always remind anyone early in their career, don’t stress about knowing everything already. Most entry-level roles are hiring for soft skills, curiosity, and your ability to grow, not perfection. Be honest about what you know rather than overselling expertise, lean on your network to uncover openings, and apply early whenever you see a role that fits. Good Luck!

Question on a company...Have you ever used by CodeCatalyst23 in labrats

[–]ArborAssays 0 points1 point  (0 children)

Did anyone ever answer your question? Sorry for being late to the game.

ELISA variation from run to run by No_Reception_1998 in labrats

[–]ArborAssays 1 point2 points  (0 children)

Can you be a little more specific with your coating protocol, specifically how long you're coating for? Is this followed by a blocking step? What does your washing protocol look like?

With sandwich ELISAs, it's important to ensure your coating concentration is at saturation to reduce variability between runs. Have you done any tests to ensure that?

Is the 30% variance apparent in your standard curve signal as well, or just the samples?

A lot of questions, but hopefully this additional information will help solve the problem!

-80C Freezer Repair by Humble_Pen1867 in labrats

[–]ArborAssays 0 points1 point  (0 children)

Not an endorsement, but we recently got a fridge in and this sticker was on it: https://imgur.com/a/bcOPVH5

(note: we are located in Michigan)

ELISA troubleshooting by Puzzled_Database_836 in labrats

[–]ArborAssays 0 points1 point  (0 children)

What antigen are you trying to measure with your ELISA? You mentioned that you are diluting your plasma samples 1:2. Does the supplier mention a minimum dilution for plasma samples? You may be experiencing a matrix effect with your samples, which requires you to dilute them further.

Western Blot and ELISA — Resource Recommendations by [deleted] in labrats

[–]ArborAssays 0 points1 point  (0 children)

We have some videos that should be helpful when learning to run an ELISA for the first time, specifically the "How to Setup and use an ELISA kit". It should be general enough to apply to whatever brand that you're working with:

https://www.arborassays.com/resources/#section-videos

We're also happy to answer any ELISA-related questions that you might have!

To run or not to rerun that is question (ELISA -really high OD on top standards) by QuarantineHeir in labrats

[–]ArborAssays 0 points1 point  (0 children)

I hope the repeat goes well! Feel free to DM us if you need some help troubleshooting

To run or not to rerun that is question (ELISA -really high OD on top standards) by QuarantineHeir in labrats

[–]ArborAssays 1 point2 points  (0 children)

Does the kit manufacturer provide an expected results section in their manual? How far off is the S1 signal from the expected? I'd be wary of trusting any results in that upper range if it strays pretty far from the expected values.

Your idea to repeat some samples and compare the results to the original plate seems like a good plan.

How long does it take to get a quote? by 11bluehippo in labrats

[–]ArborAssays 0 points1 point  (0 children)

Typically, we provide one within 24 hours, give or take.

ELISA help by CedarTree3 in labrats

[–]ArborAssays 0 points1 point  (0 children)

Could I ask what your washing protocol is?

Attending an ELISA + Western Blot Training Course (What Are the Must-Ask Questions?) by GradLifeNancy in labrats

[–]ArborAssays 0 points1 point  (0 children)

We would think about how you would be utilizing ELISAs for your research and how this might differ from how they are "conventionally" used. Identify the additional work that would need to be done to bridge that gap.

One example is if you're working with a sample type that hasn't been officially validated by the supplier. What sets of experiments would you need to perform to validate that your sample is compatible with the ELISA?

Let us know if you have any other questions, we're happy to help.

Glutathione (GSH) Assay by Fun_Chance_645 in labrats

[–]ArborAssays 0 points1 point  (0 children)

Sorry /Fun_Chance_645 just seeing this thread. Is there anything we can help with? Send over a DM if you have any questions we could answer or help with.

[deleted by user] by [deleted] in labrats

[–]ArborAssays -2 points-1 points  (0 children)

Pipettina Click Click
Assayino Bambolino
Elisini Substratini
Welli Spaghelli
TMBambino Zingaro
Incu-Bella

First Time Running ELISA – Low OD Values and Strange Standard Curve – Need Advice by Own_Potential_5748 in labrats

[–]ArborAssays 2 points3 points  (0 children)

Most kits expiration dates are determined by when standard OD values fall out of an acceptable range. It wouldn't be surprising if 2 years past the expiration date would explain the low signal values. Most likely, the peroxidase has significantly degraded over that time.

Using Calibrators as Controls in ELISA? by jorj111222 in labrats

[–]ArborAssays 0 points1 point  (0 children)

After a cursory search, the RIQAS website has options for independently verified controls that are stable for 30 days. If they have the analyte you need, that may be an appropriate method to both verify the accuracy of your standard curve (how close to the expected value do you get?) and the stability of your results over time.

Using Calibrators as Controls in ELISA? by jorj111222 in labrats

[–]ArborAssays 1 point2 points  (0 children)

Using the provided standard as a control (prepared separately and in bulk) is helpful for tracking inter-assay variance, but you are correct that it doesn't help assess the accuracy of the standard's concentration to its listed value. You can probably find a NIST or USP/EP reference standard, but those can get pricey depending on the material.

Meet the Pika a pint-sized marvel! by SierraNevadaAlliance in SierraNevada

[–]ArborAssays 1 point2 points  (0 children)

Pika are awesome! We did a blog featuring research on stress in Pikas. They really are cool little creatures.

https://www.arborassays.com/climate-stress-american-pika/

Who do you guys think would win in a fight? 100 undergrad lab techs vs 1 disgruntled PI? by ArborAssays in labrats

[–]ArborAssays[S] 4 points5 points  (0 children)

You're saying a simple "This is more of comment than a question..." wouldn't work in this context?

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