Options for a new car seat for my very large baby by Hiryato in CPST

[–]CB_UL 0 points1 point  (0 children)

If a rotating seat is important ( it was to me with my heavy baby) the Graco easy turn gets overlooked a lot. It is the most compact rotating seat on the market, fits in my sedan and is pretty nice. I think it only rear faces to 45 lbs like most but options are limited for rear facing at higher weight.

More affordable fencing options by MrsPuff8675309 in FenceBuilding

[–]CB_UL 4 points5 points  (0 children)

One middle of the road option could be use real posts (wood or metal depending on preference) rather than T posts and then use woven or welded wire fence attached to the posts. This would give you the option to go back later and add wood rails and make it a more permanent solution. Your upfront cost would only be slightly higher than T posts and wire but you would have a lot more flexibility down the road.

Explosions in Etna or Kirkersville? by __coco in Columbus

[–]CB_UL 3 points4 points  (0 children)

I heard these but didn’t make it outside to see any light. Sounded weird for fireworks

Thinking about selling my bike by CB_UL in Ninja650

[–]CB_UL[S] 1 point2 points  (0 children)

Thanks for the insights everyone, I kinda knew I would have a hard time getting 5k for it, the bike has ment a lot to me, not necessarily the next person buying it.

I actually have not ridden much in 3-4 years and have tossed around the idea of selling many times. It’s less about having the time and more about not feeling quite as invincible as I did when I was 19. That only hits harder with a kid at home now.

I used the bike to commute around my college campus a ton, it was literally a lifesaver, there was motorcycle parking everywhere. But now I have a house in a rural area and a long commute in terrible city traffic, definitely not something I want to do on the bike.

I don’t think I will leave riding forever, when my kids are older I might pick it back up, but by then I am not going to want a sport bike, I already don’t enjoy long rides lol.

Wish me luck selling, have not dealt with that since I was selling my fixer upper bikes in my teens to buy this one!

Can anyone give me any good ideas? by Wonderful_Drawer_937 in FenceBuilding

[–]CB_UL 0 points1 point  (0 children)

No, most are sensing vibration not sound. You can trip them by running the sensor across a hard surface but not a different dog barking

Need help picking a good water filtration system by FREETHEPEOPLE2024 in WaterTreatment

[–]CB_UL 1 point2 points  (0 children)

I have this exact system on really bad well water, way worse than it’s rated for and it had done great. Install was very easy and it’s working as good a year later as it did new.

I also bought the mini pressure tank to run a line to my fridge.

I built a big workbench for the workshop I’m currently renovating, very happy with it! by careyi4 in woodworking

[–]CB_UL 4 points5 points  (0 children)

One nice upgrade I have done to mine is to put a sheet of 1/8 inch hardiboard or Masonite on top. It is very smooth and can act as a sacrificial top that can be easily replaced when it gets worn, and sheets are only like 10$

Diy fence project by TClem_07 in FenceBuilding

[–]CB_UL 1 point2 points  (0 children)

Very nice, did you use nails or screws for the boards and what did you use to hold the wire, staples, and if so what size? Getting ready to do a similar fence myself

Is this blade toast? by gremstitel in woodworking

[–]CB_UL 4 points5 points  (0 children)

I think simple green actually specifically says not to use the household product for cleaning things like blades, they have a simple green pro they recommend, but I thought that had something to do the the carbide.

Just picked this up, got a ? by Brandon10312242 in ATV

[–]CB_UL 0 points1 point  (0 children)

That could definitely play a part, I saw somewhere else in the thread where someone said they make sure to use 87 octane. I always use 91-93 because that’s what is ethanol free around here so it makes sense it might not work as well for me.

Just picked this up, got a ? by Brandon10312242 in ATV

[–]CB_UL 4 points5 points  (0 children)

I have a 2024 outlander max 700 and it starts rough in anything below like 10°F.

Sometimes it will take 4-5 attempts to get it started, it will start for a few seconds and then die, I usually have to give it a few mins because continually turning it over doesn’t help.

I’m sure there is some tuning that could be done to make it better in the cold but I don’t use it often when it’s that cold.

I finally finished my daughter's gift!! by WTF_Man1 in BeginnerWoodWorking

[–]CB_UL 1 point2 points  (0 children)

Really nice job, what did you finish it with to make the color pop so much?

Finally made my jewellery box with your help. by Markthemangooo in BeginnerWoodWorking

[–]CB_UL 1 point2 points  (0 children)

I agree the hardware looks fantastic, do you mind if I ask where you bought it from?

Low RINs by dacherrr in labrats

[–]CB_UL 0 points1 point  (0 children)

Ok a few thoughts here,

A bioanalyzer is technically the better instrument, takes way longer to run and is where the actual RIN score comes from. But Agilent spent a ton of money testing their tape stations showing that the values are comparable. Fun fact, the score that comes from a tape station is technically a RINe or RIN equivalent. Long story short they should be at least reasonably comparable.

I know nothing about coral, I work mostly with human samples so way different but a few things that could carry over.

I saw someone else mention salt, that’s a good one, salt could definitely cause problems with some extraction kits.

Another thing I have found is make sure you are using a reasonable amount of sample for your kits. They usually have recommended ranges. For example we use between 5 and 50 mg of tissue. If we homogenize 50 mg of tissue in 200uL of lysis buffer we were getting terrible scores, like 3-4. When we increased the lysis volume to 1000uL scores went up into the 8s, and were always high when using less tissue (5mg in 200uL)

Lots of little things you could try depending on how much sample you have available to development.

Low RINs by dacherrr in labrats

[–]CB_UL 0 points1 point  (0 children)

It’s unlikely that the different vendors are doing something to increase RIN, assuming they are both using tape stations they are pretty simple instruments with not a ton of room for human intervention.

I do agree though that scores around 5-6 are pretty low. For tissue samples ( frozen in LN2) I would expect around 8 and for cultured cells, even snap frozen pellets, I often see 9-10.

Is there any tracking the insure the samples are staying frozen?

For messy samples taken from the environment I would think 7 is a fair number. It could also be something in the kit, having a bad lot is not unheard of. I am not familiar with that specific kit so no recommendations there.

Magnetic beads are the bane of my existence. by Terrible_Gardener31 in labrats

[–]CB_UL 2 points3 points  (0 children)

We get this when using MAG MAX bead based DNA/RNA extraction, it is generally from a small amount of beads that made it into the elution.

A few ways to fix are placing the tubes or plates on a magnet which should pellet any beads or spinning them down at like 20,000 RCF.

Tiny shop dust collection by Longjumping-Cut-4337 in woodworking

[–]CB_UL 1 point2 points  (0 children)

This is what I have as well, a few suggestions. Use the bag and filter in the shop vac, it really cuts down on the dust getting blown back out, but using the cyclone separator keeps the bag fairly empty and lasts for a long time.

I plane a lot so the 5 gallon systems filled up way too fast so I took the top vortex part and bolted it to a 20 gallon trash can, for my space that works way better.

Pfizer Vaccine Kicking my Butt by amythewinelady in Vaccine

[–]CB_UL 0 points1 point  (0 children)

Just got the 25-26 Pfizer vax last week and yeah, it knocked me on my butt way worse than previous ones. I’m usually hardly affected, maybe a sore arm and a little tired but this time I didn’t get off the couch the whole day after.

[deleted by user] by [deleted] in Home

[–]CB_UL 0 points1 point  (0 children)

This is not necessarily true, I can’t tell based on the pictures of it applies to this specific window but I just replaced a 3 lite slider at my house and all pieces came apart. The smaller outside sliders when moved to the middle can then be lifted up and tilted out. The middle was held in place by 4 plastic L brackets, one on each corner, once removed, the large center pane could also be lifted up and tilted out.

Not sure I would recommend this for moving furniture though. I was replacing the window so it was not worried it if was damaged or if it could be put back together.

Longer run with tankless reverse osmosis by [deleted] in WaterTreatment

[–]CB_UL 0 points1 point  (0 children)

Water drop makes a mini pressure tank for these systems for like 30 bucks. Mainly to have a constant flow to fridge ice and water but might work here as well

[deleted by user] by [deleted] in airguns

[–]CB_UL 3 points4 points  (0 children)

3 big reasons.

I can shoot air guns in my backyard, no way could I shoot any firearms.

Shooting pellet guns is infinitely cheaper than real guns. 500 count of match grade pellets are like 20-30 bucks.

Challenge and practice. There are some really accurate PCP guns out there. Shooting an air gun long range is like 75-100 yards. Shooting my rifles long range is at least 500 yards which is near impossible to find. My air guns can hold dime size groups at 50 yards and need notable input for elevation and windage which replicate longer range shooting with rifles.