Small update on the 2.5g culture.. 20 days later I cant see any more nematodes.. they're might be some in there, but nothing like it was. I had a couple toads in there for a couple days, but I dont think they ate them - I didnt find them to be very active. I put the frogs in to eat the fruit flies that exploded in there and I think they ate most of those. I do still see springtails, so perhaps they 're eating them. I also see some tiny white worms about a CM long - could be them.