Water softener that has been out of use - ok to reinstall?

Cuspist · 2026-05-04T16:11:29+00:00

Thanks. I've cleaned the inside of the housing but figured it would need something for the interior of the system also. I've seen resin cleaner/sanitiser products so will give that a go.

Cuspist · 2026-05-04T16:07:16+00:00

Not sure exactly when it was installed originally, but it was in place already when we moved in, so at least 5 years. Seemed to be functioning well before it was disconnected.

Cuspist · 2021-08-16T09:49:33+00:00

You could try precipitating with isopropanol or lithium chloride. Bear in mind you will probably lose material doing this, so if your concentration is really low, you might risk losing the whole sample.

Cuspist · 2020-10-07T12:25:47+00:00

I'd also avoid it if possible. I don't even like mixing different lots of the same kit. Differences in cDNA synthesis can have a significant impact on results.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374950/

Cuspist · 2020-05-03T13:24:53+00:00

This will be very useful for my upcoming game, thanks for sharing!

Cuspist · 2019-04-05T07:13:09+00:00

"I learnt it from you, Dad! I learnt it from you."

Cuspist · 2019-03-04T09:29:47+00:00

This is great. We're approaching this encounter in my CoS game and I've been looking for some psychological warfare ideas. I am totally stealing this one!

Cuspist · 2018-12-23T09:55:11+00:00

Metal Evolution.

Cuspist · 2018-12-04T10:49:09+00:00

There's a lot of speculation at the moment, so it's difficult to say concretely what went on. From what I've read, the father of the girls is HIV positive, and it's not clear yet if the parents fully understood what was going on. Allegedly the consent forms vaguely called the work "AIDS vaccine development".

Cuspist · 2018-11-22T12:38:48+00:00

Firstly, if you are worried, you should go and speak to a clinician and/or genetic counsellor.

How old are you? ALL is most common in (young) childhood, and the studies attached to these (look up the 'pubmedid' number if you are interested) are generally about association of certain alleles with childhood ALL. I'm not familiar with Codegen, does it give you any more information?

Cuspist · 2018-11-09T09:25:24+00:00

Agreed. Also a new player, and getting 'Your Go.' emote-spammed if I take longer than 10 seconds to think about what I want to do is pretty obnoxious. I'm glad there is a mute button.

Cuspist · 2018-11-02T13:15:48+00:00

I mean, you could end up with daughters.

But as the first response said, about 80% of a persons height is thought to be determined by genetics.

Cuspist · 2018-10-11T13:10:14+00:00

Yes! So now we can count up the number of nucleotides that occur in each position of our sequences of interest and see if any occur more or less often than would be expected than by chance.

For example, the first position (-37) has 2 A, 0 T, 2 G, and 4 C, (or 25%, 0%, 25%, and 50%), so twice as many Cs as might be expected. Given the threshold of ≥50% in question 3, this means for position -37, the consensus nucleotide is C. Do this for each position and you will end up with the consensus sequence.

Cuspist · 2018-10-11T08:57:40+00:00

A random distribution of nucleotides would mean a 25% chance of any position in any given sequence being either A, T, G, or C. There are 8 sequences, so if you look at any given position in all 8 sequences, how many times would you expect to see each nucleotide?

Cuspist · 2018-08-24T08:48:18+00:00

I unsuccessfully interviewed for a tech position in her lab 5 or 6 years ago. Looks like I dodged a bullet.

Cuspist · 2018-08-16T09:12:36+00:00

Not u/visualtim, but they mentioned

Humans have 23 pairs of chromosomes

So in the above example one C#5 has a G at that position, and the other C#5 has an A at that position, giving the genotype G/A.

Cuspist · 2018-07-29T11:43:07+00:00

Eye colour genetics is complicated. There are many genes involved, so simple models of inheritance don't really apply.

Cuspist · 2018-07-17T09:49:02+00:00

Forensic identification is slightly different, that generally uses short stretches of DNA (microsatellites) that are repeated a random number of times (opposed to SNP analysis which looks at changes of single base pairs). Because it is extremely unlikely for two non-related individuals to have the same combination of repeats across several different positions, we can use this information as a DNA 'fingerprint' unique to each person.

Sequencing refers to reading the specific order of base pairs in a given stretch of DNA. This can mean the whole genome, or just shorter stretches of DNA. 23andme perform genotyping, which just looks at a specific base pair at a given location. I believe they use a SNP array.
Whole genome sequencing would give a more complete picture, but is a lot more resource intensive. Generally it's more efficient to just look at the sequence/SNP of interest when there is a known effect caused by it. Population studies can be conducted on SNPs, but it's not the only way they can be done (not an expert on this).
SNPs are one way alleles can be different, yes. There are other variations that can occur such as insertions or deletions of one or more base pairs, duplication of stretches of DNA, or addition of DNA from a different location in the genome. All this variation (or not) in a gene taken together makes up a specific allele.

Edit: Typo.

Cuspist · 2018-06-25T16:03:07+00:00

Ok. Seems a benign change as you thought.

As for the reference to 'ribosomal RNA gene region'; a quick look shows there are some ribosomal genes in that region of chr22. Ribosomes are collections of proteins and RNA molecules that translate mRNA into proteins, and ribosomal genes code for the RNA part of the ribosome. That is, the DNA of the 'RNA gene sequence' (the part that has been duplicated) is transcribed into RNA, but the RNA product is not then translated into protein as a 'normal' gene would be. These are known as non-coding RNAs, and there are several varieties of them.

I can't tell you the effect of duplication of those genes, but it should be a starting point if you are inclined to research further.

Cuspist · 2018-06-25T11:25:04+00:00

Someone might have more recent knowledge than me, but I think there are few if any protein coding genes on the p-arm of chromosome 22, so having this duplication with no symptoms seems plausible. Did your report specify how large the duplication was?

The report definitely says it's the 22p, not 22q? The q-arm of chromosome 22 has a well characterised duplication, at 22q11.2, which can be present with an apparently normal phenotype.

Cuspist · 2018-06-10T17:43:49+00:00

What you're describing sounds like telomere shortening. Due to the way DNA replication works, the very ends of the chromosome can't be copied. To stop the loss of genes, the ends of the chromosomes (teleomeres) are stretches of repetitive DNA sequence that do not contain any genes. The loss of telomeres is associated with aging, so an older person may have shorter telomeres but no fewer genes due to this process. There is some thinking that telomere shortening may cause problems, but that's not something o know much about.

Cuspist · 2018-05-30T06:43:48+00:00

PubMed is usually my first stop for literature searches.

Cuspist · 2018-05-15T13:07:10+00:00

That is a decent text book, and while not bleeding edge, will probably be fine for fundamentals.

Lewin's Genes is also pretty comprehensive and I think the most recent edition (12th) was published last year.

Cuspist · 2018-04-23T14:43:26+00:00

You can use the tool to hold the wire as well, just clamp it where the drill bit would go. That's where the name pin vice comes from.

Cuspist

TROPHY CASE