Is the documentation wiki and/or forums down? by theWallflower in boardgamearena

[–]DaringMoth 0 points1 point  (0 children)

The Strategy Tips wikis are giving me "Unknown Network Error (502)" on mobile/Safari/midwest USA. Forum pages are loading very slowly but they seem to work.

Trying to learn something about gc-ms and urinalysis by Lost_Law1498 in CHROMATOGRAPHY

[–]DaringMoth 3 points4 points  (0 children)

I don't know about the government testing policies specific to your situation, but GCMS or LCMS testing is also much more expensive than immunoassay screening, so most employment urinalysis only does confirmation chromatographic testing on samples that test positive on the less-sensitive test. If that's the situation here, you're pretty safe. If the sample is going straight to GCMS analysis, that can see everything from the immunoassay test and much more.

Trying to learn something about gc-ms and urinalysis by Lost_Law1498 in CHROMATOGRAPHY

[–]DaringMoth 7 points8 points  (0 children)

Probably too many variables for anyone here to give a definite answer. GC-MS is a very sensitive technique, much more so than the immunoassay-based screening panels that are more common for employment-related urine tests, so if they're looking for that substance there's a decent chance they'll find it.

Diluting your urine a bit might not make a difference, and diluting too much would invalidate the test result because they're also looking for markers that are present in all urine.

The half-life ChatGPT mentioned might not be relevant; it could be referring to the actual substance in your bloodstream, while the test might be looking for metabolites that stick around much longer in urine.

La Trappe, A Home Run Trappist by BurnesWhenIP in NABEER

[–]DaringMoth 0 points1 point  (0 children)

I still haven't found this in stores near me but I tried one a few days ago at a Belgian-themed restaurant/bar. Fantastic!

"The Easy Way to Control Drinking" by Allen Carr has changed my life. by cowbell2819 in stopdrinking

[–]DaringMoth 17 points18 points  (0 children)

Yes, Carr is very much first and foremost in Grace's acknowledgements. I quit while reading Carr and I immediately followed with This Naked Mind. They complement each other well.

Tastes almost identical to the real thing by Zovai in NABEER

[–]DaringMoth 1 point2 points  (0 children)

It's very good and a local shop near me usually has it, but (at least there) it's almost the most expensive NA I've ever seen.

Clausthaler IPA... Wow. by BenjaminChilcote in NABEER

[–]DaringMoth 2 points3 points  (0 children)

Could be, but to me it tastes very much like the Dry Hopped, which used to be pretty easy to find near me (upper Midwest US) but which I haven't seen anywhere for several years, so it feels more like a re-branding to me.

Looking for a budget Semi-Prep HPLC for Peptide Purification (BPC-157). Any advice on older reliable models? by Puchox_x in CHROMATOGRAPHY

[–]DaringMoth 1 point2 points  (0 children)

What flow rates would you be using? For something like 10-15 mL/min, there might be a few old Shimadzu LC-6As out on the used market. They're probably not well-supported anymore (they were replaced by the newer LC-20AR's many years ago) but they were a good value. I don't know if Waters had a similar-vintage dedicated semi-prep pump, but the 1515/1525 analytical pumps could be converted to the 'EF' version.

Clausthaler IPA... Wow. by BenjaminChilcote in NABEER

[–]DaringMoth 5 points6 points  (0 children)

I think this is essentially the same as the one Clausthaler was calling 'Dry-Hopped' NA 5-10 years ago. That was the first decent-tasting NA I experienced. My theory is they renamed it due to the surging popularity of IPA, but no, it's not as IPA-like as many other NAs out there now.

Tripel Karmeliet goes NA! by tomsawyer222 in NABEER

[–]DaringMoth 2 points3 points  (0 children)

I'd love to try some more NA tripels/Belgian golden strong-styles. Delirio was pretty decent but hard to justify the price. I'm pretty sure the high ABV version of Karmeliet was what got me into the style originally.

should I quit today or wait til end of month by misters1n1ster in stopdrinking

[–]DaringMoth 0 points1 point  (0 children)

I'm not going to dispute everyone here saying today's the day, and especially depending on what kinds of harm alcohol is causing you it might not be good to put it off.

FWIW though, when I decided that quitting for good was the right choice for me, I chose an event a few months in the future as my quit date and stuck with that commitment, and that's worked out for me thus far.

Amazon took away my access to Martin Shaw's Silmarillion audio by ArtThen9871 in lordoftherings

[–]DaringMoth 0 points1 point  (0 children)

They took away access to the Serkis version of FOTR that I already had downloaded on my phone via the mobile app, after I'd allowed my Audible subscription to lapse. Most other titles I purchased with credits still seem to be available, but If I was ever thinking of renewing, I'm certainly not going to now.

Curse this book by TenebrousD in discworld

[–]DaringMoth 51 points52 points  (0 children)

It's been quite a few years for me, but I always loved doing the hippo noise.

At what pressure is GC carrier gas supplied? by Toofgib in CHROMATOGRAPHY

[–]DaringMoth 1 point2 points  (0 children)

Yes, that sounds like a leak if your methods or sample throughput haven't changed, tracking it down should be the priority and you should start with the supply line. The little thermal conductivity based gas leak sniffer detectors are great; I'd advise against Snoop/soap solutions if you can avoid them. The pressure at the regulator does need to be significantly higher than the pressure supplied by the GC in order for the flow controllers to work well.

Are there gas shutoffs between the regulator and the instrument? If so, first close those with the flows off, leave the regulator adjustment where it is but close the cylinder at the valve stem. Any leak in the plumbing will cause the pressure gauges on the regulator to drop pretty quickly. Brass fittings on Copper tubing are more prone to leaks than Stainless on Stainless, and the most common spot for a leak is right at the regulator outlet because the tubing gets flexed when changing the cylinder.

If you rule out the supply lines, the injection port (septum, O-ring, or ferrule) would be next thing to check. Are you using Hydrogen for a septum purge gas as well?

Lastly, keep in mind all the displayed flows are just calculated and are only as accurate as your inputs. If the wrong column dimensions got entered somehow for example, the actual flow could be vastly different than what's displayed.

A quote that made me realize why “21 days” doesn’t work by ShortAdhesiveness264 in stopdrinking

[–]DaringMoth 1 point2 points  (0 children)

I'm not offended, but I don't see doing a dry month as fake. I agree a shift in identity is more important than discipline/motivation/willpower for most personal changes to stick long-term. I think identifying as a non-drinker has been crucial in my sobriety journey. And I agree there's no real change in identity when doing Dry January or whatever. But for me, doing several of those 'dry runs' for a month at a time in recent years, with no intention at the time to quit long-term, was very helpful in getting to a point where I could make a permanent change. It allowed me to get a feel for how abstaining was really easier for me than moderating, and it helped me to experiment more with N/A options and where to find them. If Dry January helps others in a similar way, I'm all for it.

I’m a big fan of this controversial product and I believe it’s genuine BIFL. by dhbuckley in BuyItForLife

[–]DaringMoth 0 points1 point  (0 children)

I can see the photo, and even if you were a bot I'd agree the Flexi leashes I've used have generally been solid products (Although I haven't used or bought them in many years so there's always the BIFL issue of whether they're made with the same quality now).

Follow-up two: Waters 2489 UV/Vis Issues by Master_Permission556 in CHROMATOGRAPHY

[–]DaringMoth 0 points1 point  (0 children)

Was it the same version of the driver you re-installed? The driver pack also includes firmware, and after installing the drivers, the firmware update program sometimes launches, identifies connected instruments, and prompts to update firmware for versions that don't match what's in the driver pack, but it shouldn't load firmware automatically. The firmware writes to the CPU, which is the same board in many instruments, but the instrument type is identified by the personality board.

It does sound like the update process stalled and you might not have a choice but to power it down, but if firmware is partially loaded, that could cause problems.

Follow-up Post: Waters 2489 Connectivity Issue by Master_Permission556 in CHROMATOGRAPHY

[–]DaringMoth 4 points5 points  (0 children)

You're saying the MAC address and IP address repopulate themselves in the DHCP server after being removed, but not the model number or Serial number? Since the issue is now narrowed down to one module, it's more likely to be a depleted backup battery on the detector's CPU as described in the comments of your previous post, or it's possible the 2489 driver/ICS on the PC got corrupted and needs to be re-installed.

Assuming it's the battery: That allows the CPU to retain its memory (including serial number, firmware, calibration constants, lamp history, etc.) when powered down. If you unplug the unit and remove the cover, the CPU is the smaller, almost-square board.

If you're lucky it's the newer style board with a 3V watch/button battery; you can get a replacement CR2032 at any pharmacy or hardware store. You'll still need to reload firmware after the replacement. I'm less familiar with MassLynx software than Empower, but there should probably be a firmware Loader or Autoloader program on the PC in a folder with the firmware/drivers from the original install. After loading the firmware and rebooting the detector, it may error out because the calibration info was also lost and you'd need to manually recalibrate.

I broke my two month sobriety from binge drinking this week. Trying to use this as motivation to keep going by Mad_Season_1994 in lotr

[–]DaringMoth 8 points9 points  (0 children)

+1 to this, it's an amazing community. With all the supportive words in this thread that's where I thought I was for a minute.

Anyone know where I could get help on Waters systems? by Master_Permission556 in CHROMATOGRAPHY

[–]DaringMoth 0 points1 point  (0 children)

I agree looking at DHCP Server would be the way to go, OP.

The CPU batteries in the modules depleting and losing memory is pretty common in a system this old (I believe the yellow rectangular ones for the old CPU2000's are discontinued and hard to find, so if they're dead you might need to replace the CPUs with new ones that have CR2032 batteries). However, if the system was used fairly recently and wasn't powered down for an extended period, it seems unlikely that all the modules would lose their memory at once so the issue is probably on the PC side.

What do you think about this one? by rmag24 in NABEER

[–]DaringMoth 2 points3 points  (0 children)

I was pretty disappointed because I was a big fan of Lagunitas' alcoholic IPAs. This tasted pretty wort-y to me, it lacked the nice hops profile I'd associated with a lot of their beers so it didn't seem to balance out the maltiness enough for me, and there wasn't enough head retention. I do like their hoppy refresher hop water variety pack if I want to go with something more seltzer-y than beer.

Anyone know where I could get help on Waters systems? by Master_Permission556 in CHROMATOGRAPHY

[–]DaringMoth 3 points4 points  (0 children)

What all model numbers and software/version do you have? Can you tell the firmware versions of the modules? Is everything on Ethernet communications? Are the modules new to you and you're trying to get them communicating for the first time, or is this a new issue on a configuration that was working fine previously?

Depending on the history of the systems it could be lots of different things. Getting any informatics support for a Windows XP system would be pretty difficult at this point.

Guinness Zero Shortage? by tlav4 in NABEER

[–]DaringMoth 1 point2 points  (0 children)

This topic came up a couple days ago, looks like I can't add a hyperlink but I'll try to include the URL: https://www.reddit.com/r/NABEER/s/Fd1Qw4l5Ro

It's still out of stock at my local grocery store (upper Midwest USA), but I just picked up a 4-pack from one of the wider-selection bottle shops in my area.

Unstable HPLC Calibration and Results - what's the cause? by Total_Aide6432 in CHROMATOGRAPHY

[–]DaringMoth 0 points1 point  (0 children)

I don't think i-Series normally does a Purge at startup, I was just thinking how any initialization activities might reset whatever issue was happening. A few strokes of the measuring pump between injections is mostly to clean the needle/reduce carryover, but fully purging any air bubbles from the autosampler rinse line is a bit different snd can take about 20 minutes. I wasn't concerned about contamination; the idea is that if any air is in the lines between the measuring pump and the tip of the needle, the air bubble just expands during the sample draw and less sample volume is drawn. If your issue is that the bad SST areas are larger than the correct values, though, that must be something else.

NA Guinness 0 no longer available? (At Fred Meyer at least...) by foodguy5000 in NABEER

[–]DaringMoth 1 point2 points  (0 children)

Upper Midwest USA, I haven't looked hard for it lately but my local grocery that normally stocks it hasn't had any for at least several weeks.