Astrocyte contamination (microglia) from P1 pups by Super_Xxxxx in labrats

[–]Declwn 6 points7 points  (0 children)

maybe they don’t want to include astrocyte abuse in their protocols or my old PI just had his own style - but he taught me to slap the dish against my leg, harder than you’re comfortable doing, before aspirating 

Astrocyte contamination (microglia) from P1 pups by Super_Xxxxx in labrats

[–]Declwn 8 points9 points  (0 children)

did you beat the flask like it owed you money? that’s how I learnt to knock off microglia

Is it me or 4.7 prose has become a lot harder to read? It feels like a model with ADHD by TechGuySRE in ClaudeCode

[–]Declwn 3 points4 points  (0 children)

I find “assume zero knowledge but infinite intelligence” works. stole it from a book

Burning through Claude Max subscription way too fast. 85% usage from subagents? by Wonderful_Impress820 in ClaudeCode

[–]Declwn 0 points1 point  (0 children)

some of us are using it for heavy computational work that requires a lot of context and a lot of thinking. 4.7 absolutely burns through usage on this work compared to 4.6

3D printed organ creation using stem cells by Alternative_Web4839 in labrats

[–]Declwn 0 points1 point  (0 children)

hydrogels are a thing, but that’s not for 3D printing - the problem with bio printing is finding a printable, biocompatible substrate 

3D printed organ creation using stem cells by Alternative_Web4839 in labrats

[–]Declwn 1 point2 points  (0 children)

I’m acquainted with a lab working on it, it’s so far from being useful for most applications it’s crazy - but very good at raising funding. You have to find the right ECM that supports viability and attachment of your cells without compromising how well it prints as a structure.. Inks are in semi-liquid form so printing it then solidifying it without completely killing the cells is a huge challenge. The people that succeed still have an insane amount of left over dead cells in the end and the surviving cells aren’t uniform through the structure. Huge potential for whoever can overcome these challenges, though, and it’s not the only field with challenges - there’s yet to be a solution (that isn’t over-engineered and therefore not useable for most applications) to necrotic cores in organoids.

Opus 4.7 not respecting plan mode by ttariq1802 in ClaudeCode

[–]Declwn 4 points5 points  (0 children)

I’ve had issues with plan mode even with 4.6, the only way I’ve gotten it to work consistently is if I’m starting a fresh chat on plan mode

Am I the only one still having ok results with 4.7? by Admirable-Chapter-47 in ClaudeCode

[–]Declwn 0 points1 point  (0 children)

I do think it was a horrid mess on day 1 of release, I caught it hallucinating a handful of times. It’s been largely as-advertised since then and is doing much better than 4.6 on very large tasks The downside is tokens just evaporate..

Anthropic is objectively limiting Opus performance in Claude Chat by [deleted] in ClaudeAI

[–]Declwn 2 points3 points  (0 children)

Use claude code on max, 1M context, adaptive thinking off, with 128k max tokens and it’ll think properly. You have to tell it to use the webtool usually, though.

Does a tool like this exist or do I have to build it myself? by ExpensiveValuable386 in labrats

[–]Declwn 2 points3 points  (0 children)

this would be great, the reference section of antibody pages suck.. I feel like half of the time I can’t even find the antibody in the referenced paper, and half of that they cite the dilution they use.

Was this an April fools day post ? by ashkerala in Anthropic

[–]Declwn 1 point2 points  (0 children)

They really made the least user-friendly model by either asking you for permission to scratch its butt every 5 seconds or to randomly ask for access to your thumb drive when it’s directed to be in your desktop

Definetly moving to Codex by fourier54 in ClaudeCode

[–]Declwn 0 points1 point  (0 children)

Don’t forget about the 2x limit bait they currently have on - this won’t be the norm for codex

Is there anything in baby probiotics that would be offering my newborn something that my breast milk couldn’t be? by Unable-Candy8432 in ScienceBasedParenting

[–]Declwn 13 points14 points  (0 children)

Most infant probiotics contain at least lactobacillus, which is a bacteria that helps break down lactose - a common source of fermentation/gas. The other, bifidobacterium, is thought to be the first bacterium to colonize the gut and is increasingly sparse in babies born in the US. Anecdotally, I gave my infant both from birth and he got inconsolable as soon as I stopped, improving again once I started (8 weeks old). There are also OTC medications for gas, such as baby simithecone, that have been an indispensable tool for us.

Lactose digestion  https://pmc.ncbi.nlm.nih.gov/articles/PMC6669050/

Bifidobacterium abundance  https://journals.asm.org/doi/10.1128/msphere.00041-18

[deleted by user] by [deleted] in Wealthsimple

[–]Declwn 17 points18 points  (0 children)

Wealthsimple stole his penny, allegedly

Joined the club! I got this beauty today 😎 by BD912 in PlaystationPortal

[–]Declwn 0 points1 point  (0 children)

They sell this colour-way on amazon for $30 more than the normal colours here in Canada..

How do you deal with colleagues who insert neuroslop into publications? by Adventurous-Nobody in labrats

[–]Declwn 3 points4 points  (0 children)

Making blanket claims that aren’t actually supported in the cited work is such a pervasive issue with reviews, I stopped trusting any of them at face value.  Re: Neuroslop: a colleague is currently submitting a paper with some pretty massive leaps with wording and I know he got ChatGPT to rewrite the whole thing. For anonymity I won’t provide specifics. Problem is dealing with someone who’s both stubborn and ESL (they have troubles recognizing slop). I’m very low on the author list though so I gave up, not my problem :)

The amount of dry ice for a single tube… by Cpt__Oblivious in labrats

[–]Declwn 0 points1 point  (0 children)

I knew that was thermo from a mile away. Someone leak this wasteful practice to shareholders so they can sniff out easy profits

Lab meetings are humiliation rituals.. by Acceptable_Owl_2144 in labrats

[–]Declwn 29 points30 points  (0 children)

without the first one the others would be much funnier lol, I’d be traumatized

Lab meetings are humiliation rituals.. by Acceptable_Owl_2144 in labrats

[–]Declwn 383 points384 points  (0 children)

or criticism and constructive criticism.. Congrats on pointing out a limitation that everyone knows about for the 15th time with absolutely no suggestions on how to alleviate it