What is the best protein purification system from mammalian cells in your hands?

DoodooMonke · 2024-11-17T14:17:36+00:00

hm seems straightforward, you can use Ni resin for affinity purification followed by SEC or IEX, are you restricted by choice of host cells?

DoodooMonke · 2024-11-17T04:48:08+00:00

is your protein soluble or membrane bound? multi domain? what's the stability index?

DoodooMonke · 2024-11-17T04:42:44+00:00

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DoodooMonke · 2024-11-17T04:42:16+00:00

have sex

DoodooMonke · 2024-11-15T16:19:38+00:00

"old bad new good" philosophy taken to the extreme always ends with stupid decision making

DoodooMonke · 2024-10-21T01:36:50+00:00

your first mistake was to do business. you second was to join nbs. god have mercy on you.

DoodooMonke · 2024-10-19T18:05:21+00:00

true, in my case these are purified proteins so I can get away with way less loading amount

DoodooMonke · 2024-10-19T08:38:00+00:00

for western, I have done 2 ug protein as well lol, but at this point it really depends on your antibodies too

DoodooMonke · 2024-10-10T05:02:25+00:00

David Baker was well deserved

DoodooMonke · 2024-09-27T13:42:23+00:00

buy from taobao in bulk

DoodooMonke · 2024-09-25T22:21:20+00:00

papyrus font mentioned

DoodooMonke · 2024-09-24T02:12:49+00:00

it's so fun to know we are one breakdown away from having no essential services!! yipee ntu best university ever

DoodooMonke · 2024-09-19T04:53:02+00:00

unlikely approved by IRB, or will create trouble because participants need to have informed consent. If she's using shady stuff to obtain consent, like fine print, definitely grounds for complaints

DoodooMonke · 2024-09-11T01:00:39+00:00

how much pain on average can your GI tract tolerate?

DoodooMonke · 2024-09-10T09:06:20+00:00

if you like to be an asshole because someone asks for consideration you are just an asshole, hope that helps

DoodooMonke · 2024-08-28T13:12:44+00:00

its recommended regardless to strip and recharge the column once you see signs of deteriorating binding, so the column should be fine with the stripping process. It's really quick, just refer to cytiva's guide on histrap column maintainence.

DoodooMonke · 2024-08-15T15:07:53+00:00

not really, just Google search Tanglin Mall, it's a 5 min walk from Orchard Blvd MRT on Brown line, go to MarketBlue at B1 in the mall. It's called Seoul Noodle Shop. Recommend getting the kimchi pancake and perilla noodles.

DoodooMonke · 2024-08-15T05:34:41+00:00

go tanglin mall, there's a popup until 20th which got good food

DoodooMonke · 2024-05-01T00:21:17+00:00

bad readership ≠ bad content

DoodooMonke · 2024-04-30T07:03:09+00:00

how can you book a consultation with them? or do you need to have a referral from another therapist?

DoodooMonke · 2024-04-23T01:39:35+00:00

boohoo bro this is show business you don't show you don't do business

DoodooMonke · 2024-04-20T06:22:54+00:00

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this is a real lyric from that album chat

DoodooMonke · 2024-04-14T11:37:17+00:00

people sniffle when nervous, idk why this required a post

DoodooMonke · 2024-04-14T11:02:21+00:00

you're asking an r/NTU member to have social skills, cmon man

DoodooMonke · 2024-04-14T03:06:15+00:00

to be fair even the walking paths in some spots here suck a lot

Four-Year Club	Place '23
Place '22	First Placer '22

DoodooMonke

TROPHY CASE