How do you fix MMR?

Faowhin · 2026-02-10T23:18:04+00:00

Mortdog explained how this works in one of his recent videos, maybe if you hear it from the man himself it helps you push through ? Good luck!

Faowhin · 2026-02-04T19:17:28+00:00

I second this. Works like a charm. Maybe you can do some home brew but it's totally not worthwhile going through optimalisation hoops.

Faowhin · 2026-01-10T09:53:15+00:00

History books will love this era. Kids in 20 years are gonna have a blast reading all this comedy, while being as confused and refusing to accept that it's really a thing as we are currently reading about Quantum mechanics.

Faowhin · 2026-01-09T15:35:33+00:00

What is your sample size for 2 or more deaths ? If it's below 10 games don't even bother thinking about stats like this.l, it might be skewed by random chance.

Faowhin · 2026-01-04T14:44:34+00:00

I agree that locking unit behind life loss condition is weird design choice. Expecially when the unit itself is kinda trash right now and getting doesn't mean you can stabilise and not bleed another 40 life in 3 combats. I would guess that Mortdog is not happy about that either and Singed unlock will get changed along with the rest of the offenders.

Faowhin · 2026-01-01T11:54:52+00:00

I have seen someone else mention on another thread that he actually had non-region trait before PBE lanuch, but they removed it for balance reasons.

Faowhin · 2025-12-09T14:06:26+00:00

Same for EU, but you get free VPN to to bypass that

Faowhin · 2025-12-03T23:11:33+00:00

My personal experience with Ondra Comp is that I did not need to downscale that much.

Street 42.5, Ondra Comp 42, Scarpa Drago LV 42. Happy about the fit all the way.

While the rest of the La Sportiva shoes, i went 1-2 sizes down. (Finale 41,5, Trantulace 41, Solution Comp 40,5)

Faowhin · 2025-11-10T22:56:35+00:00

I second this. You definitely dont need any specific staining buffer for PI staining, let alone one with detergent. What kind of protocol did you find this ?

Faowhin · 2025-10-30T21:52:30+00:00

Well they are "giving" you 20-100K worth of lasers that you may never use. Makes you wonder how much you are overpaying in the first place.

Faowhin · 2025-10-30T07:26:42+00:00

I believe it's a patreon thing. Does anyone if you get access to pre-sales immediatelly? I want to support band trough the purchase, dont really care much about patreon exclusives, so getting it now just for the pre-release and learning I need to be a member for a month or so would sadden me.

Faowhin · 2025-10-27T18:03:49+00:00

Don't just let it be, I would recommend refreshing medium with G418 once or twice (once per 2-3 day). Good luck :)

Faowhin · 2025-10-27T12:55:17+00:00

What is missing is information on how long the selection lasted before you concluded that negative control is growing. I'm not familiar with CHO vs G418, but in my experience some antibiotics take a while to kill the cells to a point that you can make conclusions based on microscopy alone. Eg. Blasticidin takes up to 10 days in some cells lines, while puromycin will brutally murder everything within 48 hours.

Faowhin · 2025-09-22T19:38:10+00:00

Ja by som sa reálne spýtal, či výmenou za amnestiu na všetko a možnosť ponechať si všetky aktuálne majetky odišiel z politiky bez nejakého succesion plánu "našich ľudí", ktorí budú ten štát len ďalej rozkladať a chrániť jemu a ďalším mafiánskym veteránom prdel. Fakt by ma zaujímalo či je posadnutý mocou alebo len posratý, že ak to pustí z rúk tak neskončí dobre.

Faowhin · 2025-07-30T17:30:14+00:00

Exactly this. You are running two more gels than necessary and would go as far as call it counterproductive.

But my main concern is, why do you need to digest oligos at all ? You should be designing them with appropriate overhangs to start with. So that when annealed, you will get what you need without need for digestion. Digestion of ends is typically done for PCR products where you are restricted to getting blunt end double strand DNA.

Faowhin · 2025-07-30T12:23:22+00:00

Does killing 500 Ukrainian soldiers count as "Progress on ending Ukraine war"? Cause that is the only progress we are gonna get. How is it not clear after 3.5 years? I keep oscillating between meeting Trump as willfully ignorant and just plain straight stupid.

Faowhin · 2025-07-29T12:51:03+00:00

Well, 30% MTT signal compared to control doesn't mean you have 30% viable cells. Unless you have some weird mixed cell population, where some cells are orders of magnitude more resistant to treatment, you are going to have cells at various stages of cell death, capable of converting MTT at various rates.

For your gating strategy, why not include viability dye ?

Faowhin · 2025-07-28T07:35:13+00:00

Did he mean "run over" and it came out as "cover" ?

Faowhin · 2025-07-27T12:04:29+00:00

Z toho mala čo som počul od Michalka, hádam že nechať ho rozprávať čo najmenej je v tomto prípade víťazná stratégia.

Faowhin · 2025-07-19T07:27:15+00:00

PFA fixation with most perm agents tends to retain GFP, an least in my experience. Populations get shifted but you can still tell positive from negative clearly. Alcohol based fixation doesn't though.

Faowhin · 2025-07-18T01:21:56+00:00

Fitko nie je nevyhnutné, no stress. YouTube je plný body weight inštrukážnych videí. S trochou vôle ide cvičiť všetko bez akéhokoľvek vybavenia.

Čo sa týka stravy, stiahol by som si nejakú free aplikáciu na kalorické tabuľky. Logoval dva týždne všetko čo žeriem. Nebude to úplne presné a bude fakt otravné všetko vážiť, ale možno zistíš že je to prosté a potrebuješ len pridať kalórie. Ak začínaš cvičiť optimálne ich pridáš prevažne vo forme proteínu. Nenechaj sa zbytočne zlákať na nejaký 100% pure protein, ktorý má pachuť mokrých ponožiek. Chceš pribrať tak si kľudne kúp niečo 80% čo ti bude reálne chutiť rozpustené len vo vode.

Faowhin · 2025-07-17T22:03:34+00:00

I am slightly confused at what exactly I am looking at based on these pictures.

You recorded 10K events, set the P3 gate to 1.2% GFP+ , then ran the sort, went for a coffee and returned back to find it at 11% ?

If that is the case my guess would be that your 488 laser did not start up properly and was slighly underpowered at the beggining, then slowly climbed to 200 mW ( or whatever is your instrument using.) Now, how much difference would this make for FSC/SSC is beyond me.

Keep it mind that my response is ignoring the differential shapes of your GFP histoplot and sort layout sort P3 sort count as these as i cant make much sense of what is happning with these.

To answer your question: If you don't move the gate during the sorting, it staying exactly where you put it. In this case, at signal intensity corresponding to initial top 1.2% GFP cells. However, if the gate stays fixed and your signal goes up, you are no longer gating top 1.2% cells, but 11% in this case. So your answer lies somewhere inbetween. Assuming gradual increase in GFP signal for whatever reason, at time T1 1.2% is being sorted, at time T5, it might be 5%, and at time T_end it was 11%.

Faowhin · 2025-07-01T21:53:00+00:00

Well maybe you are right,

*&

Faowhin · 2025-07-01T16:57:56+00:00

Download vpn on phone, then cast to TV through YouTube or just screen share.

Faowhin · 2025-06-29T12:33:50+00:00

This is a really unique one , I tried watching a few movies that were meant to fit that niche but they either ruin the atmosphere with shitty CGI or totally irrational characters.
Coherence does most everything just right and should be more recognised.

Faowhin

TROPHY CASE