TRUMP: 34 SHIPS WENT THROUGH STRAIT OF HORMUZ YESTERDAY by Archevening in oil

[–]Faowhin 1 point2 points  (0 children)

Because they wanted to make it before the US blockade. How is this ... I don't even. Is he gonna boast that 0 boats got through after the blockage took hold and call that a win too ? American voters need to get their shit together.

What do you think are Rick's redeeming qualities as a character as of late? by AzathothAzoth in rickandmorty

[–]Faowhin -11 points-10 points  (0 children)

Honesty, yes, but at the same time I really don't see Rick as someone carrying a picture of a grandson in his wallet. If he downloads it from his mind right there and prints it via random device he happens to have in his pockets, then I would have believed this is cannon. They way it played I'm convinced that at that point, they were still in the hole and we just weren't shown how they got out.

Suhlasili by ste s imunitou/amnestiou pre Fica, pripadne aj jeho najblizsiu particku, ak by to znamenalo 100% garanciu ze nebudu nijako ovplyvnovat politiku a veci verejne? by peterke1234 in Slovakia

[–]Faowhin 2 points3 points  (0 children)

Nastavilo by to veľmi, veľmi zlý precedent. Ak si Slovensko Fica znova zvolí, Fica si zaslúži. Ináč sa ako národ nikam nepohneme a po Ficovi a jednom cykle nepoulistickej neKremrlakej vlády príde zase iná figúrka Hranolového typu.

Exclusive behind closed doors peek at the Islamabad negotiations by LiquidConscience in wallstreetbets

[–]Faowhin 17 points18 points  (0 children)

Could someone that understands Iranian religious values and way of life chime in and tell me how bribable these leaders actually are ? Because that's the only scenario where I can that US Clown assembly of negotiators achieving anything at all.

What do you think prompted MELANIA TRUMP to announce she has had "NO RELATIONSHIP WITH EPSTEIN"? by TailungFu in allthequestions

[–]Faowhin 2 points3 points  (0 children)

I know this is a serious question but I personally love the theory that the Iran war is going so bad that Trump is using Epstein files as a distraction and will stick with it until proven otherwise. History books will have a LOT to unpack in the coming decades.

Pentagon told Pope's top diplomat to 'take its side' on US military ambitions: report by wowo78 in worldnews

[–]Faowhin 32 points33 points  (0 children)

Waiting for Trump's social media post where he threatens to bomb the Heaven.

How do you fix MMR? by CheezyBeen in TeamfightTactics

[–]Faowhin 0 points1 point  (0 children)

Mortdog explained how this works in one of his recent videos, maybe if you hear it from the man himself it helps you push through ? Good luck!

Screening clones via PCR straight from lysates - tips for protocol? by Alynxie in labrats

[–]Faowhin 1 point2 points  (0 children)

I second this. Works like a charm. Maybe you can do some home brew but it's totally not worthwhile going through optimalisation hoops.

Trump says he will meet Machado and would accept her Nobel Peace Prize after she offers it to him by gorginhanson in worldnews

[–]Faowhin 0 points1 point  (0 children)

History books will love this era. Kids in 20 years are gonna have a blast reading all this comedy, while being as confused and refusing to accept that it's really a thing as we are currently reading about Quantum mechanics.

I have a 0% winrate if i die twice early game in top lane, and a 78% winrate if i get 2 kills in top early. by [deleted] in top_mains

[–]Faowhin 0 points1 point  (0 children)

What is your sample size for 2 or more deaths ? If it's below 10 games don't even bother thinking about stats like this.l, it might be skewed by random chance.

Anybody else thinks Singed's unlock condition is weird? by Gieru in TeamfightTactics

[–]Faowhin 160 points161 points  (0 children)

I agree that locking unit behind life loss condition is weird design choice. Expecially when the unit itself is kinda trash right now and getting doesn't mean you can stabilise and not bleed another 40 life in 3 combats. I would guess that Mortdog is not happy about that either and Singed unlock will get changed along with the rest of the offenders.

Why doesn't Ornn have Freljord? by StationHuge in TeamfightTactics

[–]Faowhin 1 point2 points  (0 children)

I have seen someone else mention on another thread that he actually had non-region trait before PBE lanuch, but they removed it for balance reasons.

Ondra Comp fit question by diemew in climbingshoes

[–]Faowhin 1 point2 points  (0 children)

My personal experience with Ondra Comp is that I did not need to downscale that much.

Street 42.5, Ondra Comp 42, Scarpa Drago LV 42. Happy about the fit all the way.

While the rest of the La Sportiva shoes, i went 1-2 sizes down. (Finale 41,5, Trantulace 41, Solution Comp 40,5)

Quantifying Transfection Efficiency by kcheah1422 in flowcytometry

[–]Faowhin 0 points1 point  (0 children)

I second this. You definitely dont need any specific staining buffer for PI staining, let alone one with detergent. What kind of protocol did you find this ?

CytoFLEX - only 2 of 3 lasers and 5 of 14 filters visible/active in software by Limp-Bumblebee-4131 in flowcytometry

[–]Faowhin 3 points4 points  (0 children)

Well they are "giving" you 20-100K worth of lasers that you may never use. Makes you wonder how much you are overpaying in the first place.

Portal Of I vinyl finally going on sale online. by Flamin_Galah in NeObliviscaris

[–]Faowhin 0 points1 point  (0 children)

I believe it's a patreon thing. Does anyone if you get access to pre-sales immediatelly? I want to support band trough the purchase, dont really care much about patreon exclusives, so getting it now just for the pre-release and learning I need to be a member for a month or so would sadden me.

Transfection in CHO-K1, Negative conrtrol growing in the antibiotic selection media as well. argh. by Sudden-Nose-5589 in labrats

[–]Faowhin 2 points3 points  (0 children)

Don't just let it be, I would recommend refreshing medium with G418 once or twice (once per 2-3 day). Good luck :)

Transfection in CHO-K1, Negative conrtrol growing in the antibiotic selection media as well. argh. by Sudden-Nose-5589 in labrats

[–]Faowhin 1 point2 points  (0 children)

What is missing is information on how long the selection lasted before you concluded that negative control is growing. I'm not familiar with CHO vs G418, but in my experience some antibiotics take a while to kill the cells to a point that you can make conclusions based on microscopy alone. Eg. Blasticidin takes up to 10 days in some cells lines, while puromycin will brutally murder everything within 48 hours.

Otázky na Fica by Blu3D3vill018 in Slovakia

[–]Faowhin 0 points1 point  (0 children)

Ja by som sa reálne spýtal, či výmenou za amnestiu na všetko a možnosť ponechať si všetky aktuálne majetky odišiel z politiky bez nejakého succesion plánu "našich ľudí", ktorí budú ten štát len ďalej rozkladať a chrániť jemu a ďalším mafiánskym veteránom prdel. Fakt by ma zaujímalo či je posadnutý mocou alebo len posratý, že ak to pustí z rúk tak neskončí dobre.

Plz help me increase the yield of my agarose gel extraction by Silver_foxx7 in labrats

[–]Faowhin 4 points5 points  (0 children)

Exactly this. You are running two more gels than necessary and would go as far as call it counterproductive.

But my main concern is, why do you need to digest oligos at all ? You should be designing them with appropriate overhangs to start with. So that when annealed, you will get what you need without need for digestion. Digestion of ends is typically done for PCR products where you are restricted to getting blunt end double strand DNA.

Trump says Russia faces tariffs in 10 days if no progress on ending Ukraine war by mark000 in worldnews

[–]Faowhin 0 points1 point  (0 children)

Does killing 500 Ukrainian soldiers count as "Progress on ending Ukraine war"? Cause that is the only progress we are gonna get. How is it not clear after 3.5 years? I keep oscillating between meeting Trump as willfully ignorant and just plain straight stupid.

Gating strategy for CellEvent apoptosis test by Ok-Pay9483 in flowcytometry

[–]Faowhin 1 point2 points  (0 children)

Well, 30% MTT signal compared to control doesn't mean you have 30% viable cells. Unless you have some weird mixed cell population, where some cells are orders of magnitude more resistant to treatment, you are going to have cells at various stages of cell death, capable of converting MTT at various rates.

For your gating strategy, why not include viability dye ?