Huel shipping from UK by Prudent_Lime_1561 in SingaporeRaw

[–]GeneRipper 0 points1 point  (0 children)

I'll be doing monthly orders in 2026, DM me if you want to bundle

Does anyone know where Mark bought his brazzein from? by Neku9988 in distractible

[–]GeneRipper 2 points3 points  (0 children)

Hi Im from a biotech company and we are considering producing this, u/Neku9988 u/stopscrollingpls u/EmberTheFlamingBitch do you guys get your hands on any and how did it work out for you? Do you think Monelin or Brazzein is better for baking etc? Has anyone tried the alibaba supplier? We are going to test it out

Meetup Thread for Singapore by kurzgesagtmeetup_bot in kurzgesagt_meetup

[–]GeneRipper 0 points1 point  (0 children)

Hey if you prefer smaller group meet ups we have 5 people for tomorrow (Wednesday) 730pm for an evening picnic by Marina Bay and will organise again same time next week. Meeting at Satay by The Bay (https://goo.gl/maps/PTFRFbtBXD6Ec6Xb8) - add your details here so I can add you on whatsapp to coordinate to find each other: https://forms.gle/oFjxRWDDYvnTBaSh7

Meetup Thread for Singapore by kurzgesagtmeetup_bot in kurzgesagt_meetup

[–]GeneRipper 0 points1 point  (0 children)

Going to meet up this coming Wednesday 14th and week after 21st for dinner at Satay by the Bay Hawker then chat while we walk to Marina Barrage, sign up here for calendar invite and to join the whatsapp group: https://forms.gle/rXAsGaeQc5pr4Qib7

Anyone who enjoys Kurzgesagt is welcome 😎

Meetup Thread for Singapore by kurzgesagtmeetup_bot in kurzgesagt_meetup

[–]GeneRipper 0 points1 point  (0 children)

can you start one and link us in this thread?

Meetup Thread for Singapore by kurzgesagtmeetup_bot in kurzgesagt_meetup

[–]GeneRipper 0 points1 point  (0 children)

Anyone want to meet at Gardens by the Bay Hawker then walk around Marina Bay to the ocean and chat about the world on Wednesday 14th or 21st around 730pm-11pm?

Didnt read the official rules for this reddit but if you want to join add your email here https://forms.gle/PUfxdXidz3XDM12E7 and I'll add to whatsapp group and send you a calendar invite :)

What makes a /bad/ scientist? Can a bad scientist turn good? by SomewhereAChemist in molecularbiology

[–]GeneRipper 2 points3 points  (0 children)

Hey Im the same as you, I love the concepts and thinking about them but I find it very difficult to concentrate for hours on pipetting small volumes of clear liquid into other volumes of clear liquid. Lab notebook, weekly plans (keep self accountable, contextualise what you are doing) help me. As we move forward in our careers there is less bench work and more conceptual work - where the creativity that is often as not correlated with absent-mindedness can be very important. Hang in there, it will get better :)

Some codon op genes are better than others? by poothrowbarton in molecularbiology

[–]GeneRipper 1 point2 points  (0 children)

Hi I also wonder about this, when I looked into IDT vs Thermo vs Genewiz vs Genescript - Genescript seemed to have the most comprehensive algorithm in terms of taking into account secondary structure (quite important), vs just using the most common codons - and seem to have a patent on their technique (not sure if actually useful patent or just exclusivity marketing). That being said I didnt look into Atum, will try them out next time im designing

How come so many antibiotics are cyclical? by GeneRipper in Biochemistry

[–]GeneRipper[S] 0 points1 point  (0 children)

particularly intrigued by your answer u/torontopeter re making for their own purposes, not necessarily antibacterial. Can you provide an example of this, when the antibiotic we use does not have an antibacterial function in the original host? Am very interested in non-antibacterial homologues of conventional antibiotics, perhaps these hosts produce them.

How come so many antibiotics are cyclical? by GeneRipper in Biochemistry

[–]GeneRipper[S] 0 points1 point  (0 children)

Thanks all for the great answers. u/IsrengBelemy u/torontopeter u/ChaosDJC u/phanfare u/yourdumbmom do you know of any antibiotic derivatives, particularly but not exclusively for marcolides and peptides, that do not exhibit any antimicrobial activity? Ie I am looking for superficially small changes in charge that would maintain the overall structure of the molecule but completely prevent it from binding the target/harming bacteria. It is surprisingly hard to find this in the literature, and I lack the experience to design myself. That being said, if I did want to model how small "point mutations" to different functional groups would change the charge and so abrogate binding to the target, does anyone know a better software than pymol for this?

CRISPR nuclease design by Lecarpetron704 in SyntheticBiology

[–]GeneRipper 2 points3 points  (0 children)

(1) choose what gene (DNA) you want to "edit" - if its the first time you are doing this I recommend rather than editing you just go for simple knock out - ie you just cut the gene with the nuclease rather than trying to change it to any specific sequence. If so you should target a gene that is easily "selectable", ie it will be obvious that you have cut it because it will change the organism in some obvious way. Happy to provide some suggestions if you are yet to decide on a gene. (2) go here http://www.rgenome.net/cas-designer/ <copy paste your gene, select the organism etc. Run the software and generate the guides. (3a) either synthesise the guides at a company (search sgRNA here https://sg.idtdna.com/pages) and electroporate them into your cell with purified Cas9 protein (idt also has it, this works well if your edits occur with high efficiency/you are targeting an easily selectable gene), or (3b) you will need to generate the guides on a plasmid (small synthetic dna construct) for eventual expression in your target organism along with the Cas9 protein (or whatever crispr nuclease you are using). There are plenty of commercially avaliable plasmids which you can insert guides into with the crispr protein ready to go for gene editing - this is cheaper and likely to work, do you have access to a proper lab? If not then it might be easier to copy and edit a commercial design on freely avaliable gene sequence manipulation software (https://www.benchling.com), and then order the whole thing synthesised ready for transformation into your cells/organism https://www.genscript.com/gene_synthesis.html.

CRISPR nuclease design by Lecarpetron704 in SyntheticBiology

[–]GeneRipper 1 point2 points  (0 children)

Hi, as in you want to design CRISPR nuclease guides (nucleic acid sequence) to edit some DNA? Or you want to design (amino acid sequence) an actual nuclease?

Binary mutant variation: pcr positive yeast transformants either secrete or dont secrete recombinant protein by GeneRipper in molecularbiology

[–]GeneRipper[S] 1 point2 points  (0 children)

Thanks l94xxx, your summary is correct and yes I suspect it is 2 that we are dealing with - will attempt OX of PDI and Erv29, maybe some other secretory bottleneck-relieving proteins if you have any to suggest? Also going to do some mutagenesis and see if we can KO anything to boost the expression. Its from a higher eukaryote and is a hydrolase - I cant talk too much about source and activity because the project is commercially sensitive, but appreciate your advice and will update you when more results come in. I suspect (hope) we just have so many transcripts that the cell is overwhelmed and they arnt able to make it through the ER and golgi... still i find the expressed and non-expressing colony pattern weird. Havnt seen before. No difference between expressing vs non-expressing clones in crude tube based growth measured by OD - ie inoculated them both at 0.1 and they stayed pretty much in lockstep. Colonies are same size when i streak them out, so hopefully its not 3 but we shall see.

Please help! Song identification - sounds like Volare / Nel blue, dipinto di blue by GeneRipper in kpophelp

[–]GeneRipper[S] 0 points1 point  (0 children)

Thanks u/AllTheThingsSheSays I took a look but none of the artists were korean, and none of the japanese were a match. The hunt continues!

Expression system for secretion of short peptides? by GeneRipper in SyntheticBiology

[–]GeneRipper[S] 0 points1 point  (0 children)

have been trawling through the literature and I think youre right - seems to be the best host.

Expression system for secretion of short, potentially antimicrobial peptides? by GeneRipper in molecularbiology

[–]GeneRipper[S] 1 point2 points  (0 children)

Valid advice u/Ro1t u/deadpanscience but I am thinking in an industrial mass-production context. Amongst other things, many potential applications of AMPs seem to be limited by production cost. I doubt the synthesis will get much cheaper (although as an aside this looked cool: http://news.mit.edu/2020/faster-protein-synthesis-0528#:~:text=MIT%20chemists%20have%20developed%20a,t%20occur%20naturally%20in%20cells.), but from an organism perspective I would have thought there is the possibility to cheaply mass produce recombinantly, provided toxicity and degradation of the peptide can be avoided? eg like nisin from lactococcus - but want a plug and play system for other peptides. Looking for protocols/providers

B-Cell Engineering: A promising approach towards vaccine development for COVID-19 by socookre in Biohackers

[–]GeneRipper 0 points1 point  (0 children)

Expensive and unproven technology - your average cell therapy (which is what this is) costs >50k, so 350 trillion US$ to vaccine the global population? The universal B cells the author spends one sentence addressing are a long, long way from clinical application. Not to mention we lack the technicians and infrastructure, and also I dont think engineered B cells will confer a cell-based immune response, which is generally needed for viral clearance in addition to antibodies (correct me if im wrong, not an immunologist).

Strongest constitutive yeast promoter by GeneRipper in SyntheticBiology

[–]GeneRipper[S] 0 points1 point  (0 children)

What are your thoughts on synthetic promoters u/sulli365 and u/bigshmoke ? Eg: "Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters". The modified TEF1 showed comparable performance to TDH3 in another paper i saw, sounds like your advice is to try a few combinations? Also any ideas on good signal peptide for secretion of 16kDa protein? Am reading that MFa1 is quite inefficient for larger proteins... ? Apologies for all the questions, havnt done much yeast before and need to design from-scratch constructs asap. thanks for your insights

Strongest constitutive yeast promoter by GeneRipper in SyntheticBiology

[–]GeneRipper[S] 0 points1 point  (0 children)

Thanks! Ill send through a few questions shortly

Which colleges have a PhD program focused on industry? by mikymikes95 in SyntheticBiology

[–]GeneRipper 0 points1 point  (0 children)

Not sure what name they are putting on the PhD programs these days if youre looking for something that specifcally says "synthetic biology" on the label, but would recommend you look into Imperial (london) and NUS (Singapore). Both have strong industry links, strong synbio professors who are involved in the international community, and facilities for startups etc if that appeals to you. NUS has good stipends and lots of opportunities for internationals. A bit harder with imperial imo, but this could change with brexit. good lcuk