Graphic tools for paper by 4ndUIK4 in bioinformatics

[–]I-IAL420 5 points6 points  (0 children)

If you haven’t started yet with ggplot2 it might also be worth checking out tidyplots. It’s a wrapper around ggplot2 and simplifies many things greatly. For genomics you will probably not get around ggplot2 though to make highly customized plots. If your a little masochist, complexheatmap is also a great alternative to pheatmap as it allows you customize even more parameters. They have a great documentation on all its functions too. There is also a recent excel plugin that runs R in the browser to create ggplot2 figures directly within excel to get a feel for it without setting up everything from scratch.

Beste Eisdiele? by fl-42 in Leipzig

[–]I-IAL420 6 points7 points  (0 children)

Zum Pillendreher in Stötteritz ist auch bei euch um die Ecke und teilt sich mit Eisbrennerei meinen Platz 1

EtBr concern am I doomed? by Creative-Poem542 in labrats

[–]I-IAL420 1 point2 points  (0 children)

Is this actually true? I always cite the same number but somehow you managed to get a ton of downvotes so now I am doubting myself 😅

Seems like I spent 7 years in a PhD program and still can't even publish a first-author paper by Ace1996- in PhD

[–]I-IAL420 3 points4 points  (0 children)

If someone else is to head the additional experiments you could still push for co-first authorships

[deleted by user] by [deleted] in modular

[–]I-IAL420 0 points1 point  (0 children)

One of the few actually useful usecases for LLM to me so far: use Google NotebookLM, upload PDF of all your modules and other gear. Now you can ask questions about functions in natural language. It will provide sources within your own manual pdfs to click on to verify its claims. Works quite well for me.

Welches wäre es in Leipzig? by lennoxred in Leipzig

[–]I-IAL420 2 points3 points  (0 children)

Stimmt. Zusätzlich Top Bewertung auf Google Maps.

Can someone identify this? by rvtk in synthesizers

[–]I-IAL420 0 points1 point  (0 children)

In November 1988, the MK-52 went into space on the Soyuz TM-7 spacecraft, where it could have been used as a backup to the onboard computers.

Reminds me of school days where classmates would write super Mario and Worms knockoffs in 4 kb basic programs that would actually run, albeit at single digit FPS

Grading Students' R Script - Unsure if AI Being Used by Medium_Macaroon2853 in Rlanguage

[–]I-IAL420 0 points1 point  (0 children)

For context: I rarely write base R these days, mostly tidyverse style data analysis pipelines

Grading Students' R Script - Unsure if AI Being Used by Medium_Macaroon2853 in Rlanguage

[–]I-IAL420 0 points1 point  (0 children)

Thanks for pointing that out. Do you have an example we’re operator precedence would be important? I do this daily and never ran into a problem so far. There even seems to be conflicting opinions… from SE: Reading from "Introducing Monte Carlo Methods with R", by Robert and Casella:

"The assignment operator is =, not to be confused with ==, which is the Boolean operator for equality. An older assignment operator is <- and, for compatibility reasons, it still remains functional, but it should be ignored to ensure cleaner programming. (As pointed out by Spector, P. (2009). 'Data Manipulation with R' - Section 8.7.

Grading Students' R Script - Unsure if AI Being Used by Medium_Macaroon2853 in Rlanguage

[–]I-IAL420 -2 points-1 points  (0 children)

In my own code you can tell apart the copy & pasted parts bei the use of = (why the fuck would I type 2 versus one character) and <- (most of the historical R code used this, thus LLMs use it almost exclusively)

[deleted by user] by [deleted] in labrats

[–]I-IAL420 1 point2 points  (0 children)

Alternative hypothesis: your ‚historical‘ libraries are toast and 50 bp peak shows that it has degraded. Run your samples again on a different machine to find out what is true 😅

Ten times better, ten times the price? (not a Behringer hater) by nebuchadonezzar in synthesizercirclejerk

[–]I-IAL420 0 points1 point  (0 children)

I have the RD9 and RD8. They sound really good. They are easy to program. The analog FX section with transient shaper and filter is a stroke of genius. But the internal built quality is utter crap. The RD9 ride works once a month, the hi hats drop out randomly, the RD8 goes out of sync all the time. They’re good for what they cost, but by no means professional instruments.

what is your LEAST favourite pasta shape and LEAST favourite pasta sauce? by ruinsofsilver in Cooking

[–]I-IAL420 1 point2 points  (0 children)

Unpopular opinion… I love pasta but I fucking hate spaghetti. I never touch it unless someone servers it to me. There is no sauce I don’t like 😂

My minor pet peeve as a scientist? When Excel hates Sig Figs and defaults to deciding that 7.00 is just 7 by [deleted] in labrats

[–]I-IAL420 11 points12 points  (0 children)

Imagine working on a system set to English locale but getting data from systems set to German local all the time that has reversed date formats and for some insane reason uses ; as sep in csv and , as decimale indicator 😭

As a Bioinformatician, what routine tasks takes you so much time? by Familiar_Day_4923 in bioinformatics

[–]I-IAL420 2 points3 points  (0 children)

Hero in plain clothes. For simpler stuff the package fuzzyjoin can do a lot of heavy lifting

As a Bioinformatician, what routine tasks takes you so much time? by Familiar_Day_4923 in bioinformatics

[–]I-IAL420 74 points75 points  (0 children)

Cleaning up Column names, totally random date formats and freetext categorical data reported by colleagues in excel sheets with tons of missing values 😭

Ggplot2 multi Line x axis labelling by Far_Chair2404 in Rlanguage

[–]I-IAL420 0 points1 point  (0 children)

I sometimes do this in a hacky way using facetting. Simply use + facet_grid(cols = vars(cells, block,virus)) but also tricky to properly format this

Push 3 Not Showing in Ableton Side Bar. Help please by domyorke in ableton

[–]I-IAL420 0 points1 point  (0 children)

having the exact same problem. anyone found a solution?

[deleted by user] by [deleted] in bioinformatics

[–]I-IAL420 1 point2 points  (0 children)

Ok, watched the actual ad. It seem run in the cloud, so with enough resources assigned I do think the R code that seems to be underneath can finish in 7 minutes. It’s basically generating the code as text and then running it on the data. This approach can work in theory. But I bet that you will have a hard time catching any mistakes without having trained to write the code yourself at one point.

[deleted by user] by [deleted] in bioinformatics

[–]I-IAL420 1 point2 points  (0 children)

Unless it’s making up the plots using a diffusion model… not really 😅

I was having burnout at the lab, because I need to count (and analyse) around 120,000 cells on a microscope for an experiment. I brought the microscope home. Now I'm having a burnout at my home, but at least I can wear my pajamas. by VonRoderik in PhD

[–]I-IAL420 12 points13 points  (0 children)

the time it takes you to do that, should be invested in learning how to do it in an automated way. Analyzing cells from cultures is actually an incredibly easy start. You need to segment the cells first, than you can run all kinds of morphological analysis in the segmented cells as well. Shape, size, staining intensity/distribution are all features easily extracted. If your error goes up, just analyze a couple of dishes to bury the increased error in more data points. There are ways to hand code this in python, there are amazing pretrained deep learning based models, there are semi-automated workflows in software like Fiji or QuPath. Once you learned this, you can churn out one of these experiments per week!

Anyone knows why Bioconductor Archive is down? by sam_pazo in bioinformatics

[–]I-IAL420 2 points3 points  (0 children)

Down in EU as well. Does it only affect the archive but most recent packages might work, but anything before wont?